Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons
<i>Inula britannica</i> var. chinensis (IBC) has been used as a traditional medicinal herb to treat inflammatory diseases. Although its anti-inflammatory and anti-oxidative effects have been reported, whether IBC exerts neuroprotective effects and the related mechanisms in cortical neuro...
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MDPI AG
2021-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/3/375 |
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| author | Jin Young Hong Hyunseong Kim Junseon Lee Wan-Jin Jeon Seung Ho Baek In-Hyuk Ha |
| author_facet | Jin Young Hong Hyunseong Kim Junseon Lee Wan-Jin Jeon Seung Ho Baek In-Hyuk Ha |
| author_sort | Jin Young Hong |
| collection | DOAJ |
| description | <i>Inula britannica</i> var. chinensis (IBC) has been used as a traditional medicinal herb to treat inflammatory diseases. Although its anti-inflammatory and anti-oxidative effects have been reported, whether IBC exerts neuroprotective effects and the related mechanisms in cortical neurons remain unknown. In this study, we investigated the effects of different concentrations of IBC extract (5, 10, and 20 µg/mL) on cortical neurons using a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced injury model. Our results demonstrate that IBC can effectively enhance neuronal viability under in vitro-modeled reaction oxygen species (ROS)-generating conditions by inhibiting mitochondrial ROS production and increasing adenosine triphosphate level in H<sub>2</sub>O<sub>2</sub>-treated neurons. Additionally, we confirmed that neuronal death was attenuated by improving the mitochondrial membrane potential status and regulating the expression of cytochrome c, a protein related to cell death. Furthermore, IBC increased the expression of brain-derived neurotrophic factor and nerve growth factor. Furthermore, IBC inhibited the loss and induced the production of synaptophysin, a major synaptic vesicle protein. This study is the first to demonstrate that IBC exerts its neuroprotective effect by reducing mitochondria-associated oxidative stress and improving mitochondrial dysfunction. |
| first_indexed | 2024-03-09T05:46:40Z |
| format | Article |
| id | doaj.art-f2d0fb52edd34958b5763e82b0e77868 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T05:46:40Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-f2d0fb52edd34958b5763e82b0e778682023-12-03T12:21:18ZengMDPI AGAntioxidants2076-39212021-03-0110337510.3390/antiox10030375Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical NeuronsJin Young Hong0Hyunseong Kim1Junseon Lee2Wan-Jin Jeon3Seung Ho Baek4In-Hyuk Ha5Jaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, KoreaCollege of Korean Medicine, Dongguk University, 32 Dongguk-ro, Ilsandong-gu, Goyang-si 10326, KoreaJaseng Spine and Joint Research Institute, Jaseng Medical Foundation, Seoul 135-896, Korea<i>Inula britannica</i> var. chinensis (IBC) has been used as a traditional medicinal herb to treat inflammatory diseases. Although its anti-inflammatory and anti-oxidative effects have been reported, whether IBC exerts neuroprotective effects and the related mechanisms in cortical neurons remain unknown. In this study, we investigated the effects of different concentrations of IBC extract (5, 10, and 20 µg/mL) on cortical neurons using a hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)-induced injury model. Our results demonstrate that IBC can effectively enhance neuronal viability under in vitro-modeled reaction oxygen species (ROS)-generating conditions by inhibiting mitochondrial ROS production and increasing adenosine triphosphate level in H<sub>2</sub>O<sub>2</sub>-treated neurons. Additionally, we confirmed that neuronal death was attenuated by improving the mitochondrial membrane potential status and regulating the expression of cytochrome c, a protein related to cell death. Furthermore, IBC increased the expression of brain-derived neurotrophic factor and nerve growth factor. Furthermore, IBC inhibited the loss and induced the production of synaptophysin, a major synaptic vesicle protein. This study is the first to demonstrate that IBC exerts its neuroprotective effect by reducing mitochondria-associated oxidative stress and improving mitochondrial dysfunction.https://www.mdpi.com/2076-3921/10/3/375<i>Inula britannica</i> var. chinensishydrogen peroxidebrain-derived neurotrophic factornerve growth factorneuroprotectionsynaptophysin |
| spellingShingle | Jin Young Hong Hyunseong Kim Junseon Lee Wan-Jin Jeon Seung Ho Baek In-Hyuk Ha Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons Antioxidants <i>Inula britannica</i> var. chinensis hydrogen peroxide brain-derived neurotrophic factor nerve growth factor neuroprotection synaptophysin |
| title | Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons |
| title_full | Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons |
| title_fullStr | Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons |
| title_full_unstemmed | Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons |
| title_short | Neurotherapeutic Effect of <i>Inula britannica</i> var. Chinensis against H<sub>2</sub>O<sub>2</sub>-Induced Oxidative Stress and Mitochondrial Dysfunction in Cortical Neurons |
| title_sort | neurotherapeutic effect of i inula britannica i var chinensis against h sub 2 sub o sub 2 sub induced oxidative stress and mitochondrial dysfunction in cortical neurons |
| topic | <i>Inula britannica</i> var. chinensis hydrogen peroxide brain-derived neurotrophic factor nerve growth factor neuroprotection synaptophysin |
| url | https://www.mdpi.com/2076-3921/10/3/375 |
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