Nitrate addition inhibited methanogenesis in paddy soils under long-term managements
Rice fields are a major source of atmospheric methane (CH4). Nitrate has been approved to inhibit CH4 production from paddy soils, while fertilization as well as water management can also affect the methanogenesis. It is unknown whether nitrate addition might result in shifts in the methanogenesis a...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2018-08-01
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| Series: | Plant, Soil and Environment |
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| Online Access: | https://pse.agriculturejournals.cz/artkey/pse-201808-0006_nitrate-addition-inhibited-methanogenesis-in-paddy-soils-under-long-term-managements.php |
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| author | Jun WANG Tingting XU Lichu YIN Cheng HAN Huan DENG Yunbin JIANG Wenhui ZHONG |
| author_facet | Jun WANG Tingting XU Lichu YIN Cheng HAN Huan DENG Yunbin JIANG Wenhui ZHONG |
| author_sort | Jun WANG |
| collection | DOAJ |
| description | Rice fields are a major source of atmospheric methane (CH4). Nitrate has been approved to inhibit CH4 production from paddy soils, while fertilization as well as water management can also affect the methanogenesis. It is unknown whether nitrate addition might result in shifts in the methanogenesis and methanogens in paddy soils influenced by different practices. Six paddy soils of different fertilizer types and groundwater tables were collected from a long-term experiment site. CH4 production rate and methanogenic archaeal abundance were determined with and without nitrate addition in the microcosm incubation. The structure of methanogenic archaeal community was analysed using the PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) and pyrosequencing. The results showed that nitrate addition significantly decreased the CH4 production rate and methanogenic archaeal abundance in all six paddy soils by 70-100% and 54-88%, respectively. The quantity, position and relative intensity of DGGE bands exhibited differences when nitrate was added. Nitrate suppressed the growth of methanogenic archaeal species affiliated to Methanosaetaceae, unidentified Euryarchaeota, Thaumarchaeota and Methanosarinaceae. The universal inhibition of nitrate addition on the methanogenesis and methanogens can be adopted as a practice of mitigating CH4 emission in paddy soils under different fertilization and water managements. |
| first_indexed | 2024-04-10T08:09:08Z |
| format | Article |
| id | doaj.art-f2d17eea5434410bb5d5aec90c2cd40f |
| institution | Directory Open Access Journal |
| issn | 1214-1178 1805-9368 |
| language | English |
| last_indexed | 2024-04-10T08:09:08Z |
| publishDate | 2018-08-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant, Soil and Environment |
| spelling | doaj.art-f2d17eea5434410bb5d5aec90c2cd40f2023-02-23T03:46:39ZengCzech Academy of Agricultural SciencesPlant, Soil and Environment1214-11781805-93682018-08-0164839339910.17221/231/2018-PSEpse-201808-0006Nitrate addition inhibited methanogenesis in paddy soils under long-term managementsJun WANG0Tingting XU1Lichu YIN2Cheng HAN3Huan DENG4Yunbin JIANG5Wenhui ZHONG6Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, P.R. ChinaJiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, P.R. ChinaCollege of Resources and Environment, Hunan Agricultural University, Changsha, P.R. ChinaJiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, P.R. ChinaJiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, P.R. ChinaJiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, P.R. ChinaJiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Nanjing Normal University, Nanjing, P.R. ChinaRice fields are a major source of atmospheric methane (CH4). Nitrate has been approved to inhibit CH4 production from paddy soils, while fertilization as well as water management can also affect the methanogenesis. It is unknown whether nitrate addition might result in shifts in the methanogenesis and methanogens in paddy soils influenced by different practices. Six paddy soils of different fertilizer types and groundwater tables were collected from a long-term experiment site. CH4 production rate and methanogenic archaeal abundance were determined with and without nitrate addition in the microcosm incubation. The structure of methanogenic archaeal community was analysed using the PCR-DGGE (polymerase chain reaction denaturing gradient gel electrophoresis) and pyrosequencing. The results showed that nitrate addition significantly decreased the CH4 production rate and methanogenic archaeal abundance in all six paddy soils by 70-100% and 54-88%, respectively. The quantity, position and relative intensity of DGGE bands exhibited differences when nitrate was added. Nitrate suppressed the growth of methanogenic archaeal species affiliated to Methanosaetaceae, unidentified Euryarchaeota, Thaumarchaeota and Methanosarinaceae. The universal inhibition of nitrate addition on the methanogenesis and methanogens can be adopted as a practice of mitigating CH4 emission in paddy soils under different fertilization and water managements.https://pse.agriculturejournals.cz/artkey/pse-201808-0006_nitrate-addition-inhibited-methanogenesis-in-paddy-soils-under-long-term-managements.phporyza sativa l.mineral fertilizerorganic manurebiomethanationarchaeal 16s rrna gene |
| spellingShingle | Jun WANG Tingting XU Lichu YIN Cheng HAN Huan DENG Yunbin JIANG Wenhui ZHONG Nitrate addition inhibited methanogenesis in paddy soils under long-term managements Plant, Soil and Environment oryza sativa l. mineral fertilizer organic manure biomethanation archaeal 16s rrna gene |
| title | Nitrate addition inhibited methanogenesis in paddy soils under long-term managements |
| title_full | Nitrate addition inhibited methanogenesis in paddy soils under long-term managements |
| title_fullStr | Nitrate addition inhibited methanogenesis in paddy soils under long-term managements |
| title_full_unstemmed | Nitrate addition inhibited methanogenesis in paddy soils under long-term managements |
| title_short | Nitrate addition inhibited methanogenesis in paddy soils under long-term managements |
| title_sort | nitrate addition inhibited methanogenesis in paddy soils under long term managements |
| topic | oryza sativa l. mineral fertilizer organic manure biomethanation archaeal 16s rrna gene |
| url | https://pse.agriculturejournals.cz/artkey/pse-201808-0006_nitrate-addition-inhibited-methanogenesis-in-paddy-soils-under-long-term-managements.php |
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