An improved assay of gangliosides separated by thin-layer chromatography
There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did af...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1977-09-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520416086 |
| _version_ | 1819291012954062848 |
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| author | A J Yates D Thompson |
| author_facet | A J Yates D Thompson |
| author_sort | A J Yates |
| collection | DOAJ |
| description | There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did affect the recoveries, which can reach 100% when the plates are heated for 6 min at 135°C. |
| first_indexed | 2024-12-24T03:31:53Z |
| format | Article |
| id | doaj.art-f2d233f0824d4432942b2cef2d049e2a |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-24T03:31:53Z |
| publishDate | 1977-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-f2d233f0824d4432942b2cef2d049e2a2022-12-21T17:17:10ZengElsevierJournal of Lipid Research0022-22751977-09-01185660663An improved assay of gangliosides separated by thin-layer chromatographyA J Yates0D Thompson1Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus OH 43210Division of Neuropathology, Department of Pathology, The Ohio State University, Columbus OH 43210There were no differences in the recoveries of ganglioside sialic acid from silica gel G thin-layer chromatograms when they were sprayed with resorcinol reagent varying in normality between 3 and 10. However, both the temperature at and the time for which the plates were heated after spraying did affect the recoveries, which can reach 100% when the plates are heated for 6 min at 135°C.http://www.sciencedirect.com/science/article/pii/S0022227520416086gangliosidesialic acidglycolipid |
| spellingShingle | A J Yates D Thompson An improved assay of gangliosides separated by thin-layer chromatography Journal of Lipid Research ganglioside sialic acid glycolipid |
| title | An improved assay of gangliosides separated by thin-layer chromatography |
| title_full | An improved assay of gangliosides separated by thin-layer chromatography |
| title_fullStr | An improved assay of gangliosides separated by thin-layer chromatography |
| title_full_unstemmed | An improved assay of gangliosides separated by thin-layer chromatography |
| title_short | An improved assay of gangliosides separated by thin-layer chromatography |
| title_sort | improved assay of gangliosides separated by thin layer chromatography |
| topic | ganglioside sialic acid glycolipid |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520416086 |
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