Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3
Qiang Liu,1 Shuai Wang,1 Guotian Pei,1 Yingshun Yang,1 Xianjun Min,1 Yuqing Huang,1 Jun Liu2 1Department of Thoracic Surgery, Beijing Haidian Hospital, Beijing, 100000, People’s Republic of China; 2Department of Thoracic Surgery, Peking University People’s Hospital, Beijing, 1000...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-02-01
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| Series: | Cancer Management and Research |
| Subjects: | |
| Online Access: | https://www.dovepress.com/impact-analysis-of-mir-1253-on-lung-cancer-progression-through-targete-peer-reviewed-article-CMAR |
| _version_ | 1819283770656686080 |
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| author | Liu Q Wang S Pei G Yang Y Min X Huang Y Liu J |
| author_facet | Liu Q Wang S Pei G Yang Y Min X Huang Y Liu J |
| author_sort | Liu Q |
| collection | DOAJ |
| description | Qiang Liu,1 Shuai Wang,1 Guotian Pei,1 Yingshun Yang,1 Xianjun Min,1 Yuqing Huang,1 Jun Liu2 1Department of Thoracic Surgery, Beijing Haidian Hospital, Beijing, 100000, People’s Republic of China; 2Department of Thoracic Surgery, Peking University People’s Hospital, Beijing, 100044, People’s Republic of ChinaCorrespondence: Jun Liu Email hezibei6048642@163.comObjective: This study set out to investigate the effect of miR-1253 on lung cancer progression through targeted regulation of ANXA3.Methods: RT-PCR was employed to detect the miR-1253 expression levels in lung cancer cells and its targeted gene ANXA3 mRNA determined by biological information prediction. MTT, invasion and apoptosis rate tests were employed to detect the proliferation, invasion and apoptosis rate of lung cancer cells over-expressing miR-1253 or those with low expression of ANXA3 and the expression of related proteins.Results: RT-qPCR results manifested that the miR-1253 level was down-regulated in lung cancer tissues and cells, and the ANXA3 expression increased. The miR-1253 and ANXA3 expression levels were negatively correlated. miR-1253 was correlated with tumor differentiation degree, TNM stage and lymph node metastasis of lung cancer patients. Cell tests confirmed that miR-1253 played a tumor-inhibiting function, including inhibiting proliferation and invasion of lung cancer cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that ANXA3 was the direct target of miR-1253. Moreover, after the ANXA3 expression in lung cancer cells was knocked down, proliferation and invasion of those cells were inhibited dramatically, the apoptosis rate increased markedly, and the expression levels of pro-apoptosis-related proteins Bax and caspase-3 were up-regulated, and the anti-apoptosis-related protein Bcl-2 expression was down-regulated.Conclusion: miR-1253 can inhibit the proliferation and invasion of lung cancer cells and promote their apoptosis by targeting ANXA3. It can be used as a new potential target for lung cancer treatment.Keywords: miR-1253, lung cancer, ANXA3, proliferation, invasion, apoptosis |
| first_indexed | 2024-12-24T01:36:46Z |
| format | Article |
| id | doaj.art-f2d710a9e2be466bacf32b3e9846fb1b |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-24T01:36:46Z |
| publishDate | 2021-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-f2d710a9e2be466bacf32b3e9846fb1b2022-12-21T17:22:11ZengDove Medical PressCancer Management and Research1179-13222021-02-01Volume 131767177662302Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3Liu QWang SPei GYang YMin XHuang YLiu JQiang Liu,1 Shuai Wang,1 Guotian Pei,1 Yingshun Yang,1 Xianjun Min,1 Yuqing Huang,1 Jun Liu2 1Department of Thoracic Surgery, Beijing Haidian Hospital, Beijing, 100000, People’s Republic of China; 2Department of Thoracic Surgery, Peking University People’s Hospital, Beijing, 100044, People’s Republic of ChinaCorrespondence: Jun Liu Email hezibei6048642@163.comObjective: This study set out to investigate the effect of miR-1253 on lung cancer progression through targeted regulation of ANXA3.Methods: RT-PCR was employed to detect the miR-1253 expression levels in lung cancer cells and its targeted gene ANXA3 mRNA determined by biological information prediction. MTT, invasion and apoptosis rate tests were employed to detect the proliferation, invasion and apoptosis rate of lung cancer cells over-expressing miR-1253 or those with low expression of ANXA3 and the expression of related proteins.Results: RT-qPCR results manifested that the miR-1253 level was down-regulated in lung cancer tissues and cells, and the ANXA3 expression increased. The miR-1253 and ANXA3 expression levels were negatively correlated. miR-1253 was correlated with tumor differentiation degree, TNM stage and lymph node metastasis of lung cancer patients. Cell tests confirmed that miR-1253 played a tumor-inhibiting function, including inhibiting proliferation and invasion of lung cancer cells and promoting apoptosis. Bioinformatics prediction and subsequent experiments proved that ANXA3 was the direct target of miR-1253. Moreover, after the ANXA3 expression in lung cancer cells was knocked down, proliferation and invasion of those cells were inhibited dramatically, the apoptosis rate increased markedly, and the expression levels of pro-apoptosis-related proteins Bax and caspase-3 were up-regulated, and the anti-apoptosis-related protein Bcl-2 expression was down-regulated.Conclusion: miR-1253 can inhibit the proliferation and invasion of lung cancer cells and promote their apoptosis by targeting ANXA3. It can be used as a new potential target for lung cancer treatment.Keywords: miR-1253, lung cancer, ANXA3, proliferation, invasion, apoptosishttps://www.dovepress.com/impact-analysis-of-mir-1253-on-lung-cancer-progression-through-targete-peer-reviewed-article-CMARmir-1253lung canceranxa3proliferationinvasionapoptosis |
| spellingShingle | Liu Q Wang S Pei G Yang Y Min X Huang Y Liu J Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3 Cancer Management and Research mir-1253 lung cancer anxa3 proliferation invasion apoptosis |
| title | Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3 |
| title_full | Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3 |
| title_fullStr | Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3 |
| title_full_unstemmed | Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3 |
| title_short | Impact Analysis of miR-1253 on Lung Cancer Progression Through Targeted Regulation of ANXA3 |
| title_sort | impact analysis of mir 1253 on lung cancer progression through targeted regulation of anxa3 |
| topic | mir-1253 lung cancer anxa3 proliferation invasion apoptosis |
| url | https://www.dovepress.com/impact-analysis-of-mir-1253-on-lung-cancer-progression-through-targete-peer-reviewed-article-CMAR |
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