Label-free spatially maintained measurements of metabolic phenotypes in cells

Metabolic reprogramming at a cellular level contributes to many diseases including cancer, yet few assays are capable of measuring metabolic pathway usage by individual cells within living samples. Here, autofluorescence lifetime imaging is combined with single-cell segmentation and machine-learning...

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Bibliographic Details
Main Authors: Linghao Hu, Nianchao Wang, Joshua D. Bryant, Lin Liu, Linglin Xie, A. Phillip West, Alex J. Walsh
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1293268/full