Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality
The study of the seminal plasma help us to understand the mechanisms by which reactive oxygen species (ROS) affect the sperm. The antioxidant enzymes, as the superoxide dismutase - SOD and catalase - CAT, are capable of removing the oxidative agents before they produce injuries. The aim of the curre...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Londrina
2018-03-01
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| Series: | Semina: Ciências Agrárias |
| Subjects: | |
| Online Access: | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/29761 |
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| author | Érika Aparecida Araújo dos Santos Patrícia Cunha Sousa Andreia Maria da Silva Ariana Lopes Correia Paiva Arthur Emmanuel de Araújo Lago Valéria Veras de Paula Alexsandra Fernandes Pereira Arlindo Alencar Araripe Moura Alexandre Rodrigues Silva |
| author_facet | Érika Aparecida Araújo dos Santos Patrícia Cunha Sousa Andreia Maria da Silva Ariana Lopes Correia Paiva Arthur Emmanuel de Araújo Lago Valéria Veras de Paula Alexsandra Fernandes Pereira Arlindo Alencar Araripe Moura Alexandre Rodrigues Silva |
| author_sort | Érika Aparecida Araújo dos Santos |
| collection | DOAJ |
| description | The study of the seminal plasma help us to understand the mechanisms by which reactive oxygen species (ROS) affect the sperm. The antioxidant enzymes, as the superoxide dismutase - SOD and catalase - CAT, are capable of removing the oxidative agents before they produce injuries. The aim of the current study was to investigate the activity of antioxidant enzymes SOD and CAT in seminal plasma, and their association with sperm quality in collared peccaries. Study was conducted during the dry period (August and September) on a region characterized by a semiarid climate, with an average annual temperature of 27°C and irregular rainfall (Mossoro, RN, Brazil; 5°10´S and 37°10´W). Nine ejaculates were obtained from sexually mature males (1 sample per animal) by electroejaculation. Semen was evaluated for microscopic parameters and the activity of SOD and CAT was measured by spectrophotometry. All ejaculates were white in color. Mean values for concentration were of 207 ± 160 x106 sperm/mL, motility of 83.0 ± 20.9% and viability of 72.5 ± 10.4%. In regards to the enzymatic activity, none was observed for the CAT enzyme. Trace levels of SOD (0.034 ± 0.049 AU/mgP) were detected in the ejaculates of all individuals; however, no correlation was observed between SOD levels and the sperm motility (R = 0.35; P = 0.931), vigor (R = 0.29; P = 0.133), viability (R = 0.16; P = 0.29), functional membrane (R = 0.04; P = 0.617) or morphology (R = 0.03; P = 0.637). In conclusion, we demonstrated the first description of antioxidant enzyme activity in seminal plasma of fresh ejaculates obtained from collared peccaries. SOD antioxidant activity was evident during the dry period of a semi-arid region, but no relationship between SOD and semen parameters was observed. |
| first_indexed | 2024-12-13T13:46:22Z |
| format | Article |
| id | doaj.art-f2db027f9ebe4fb6ab3adf55a58e78c3 |
| institution | Directory Open Access Journal |
| issn | 1676-546X 1679-0359 |
| language | English |
| last_indexed | 2024-12-13T13:46:22Z |
| publishDate | 2018-03-01 |
| publisher | Universidade Estadual de Londrina |
| record_format | Article |
| series | Semina: Ciências Agrárias |
| spelling | doaj.art-f2db027f9ebe4fb6ab3adf55a58e78c32022-12-21T23:43:24ZengUniversidade Estadual de LondrinaSemina: Ciências Agrárias1676-546X1679-03592018-03-0139278779610.5433/1679-0359.2018v39n2p78717001Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm qualityÉrika Aparecida Araújo dos Santos0Patrícia Cunha Sousa1Andreia Maria da Silva2Ariana Lopes Correia Paiva3Arthur Emmanuel de Araújo Lago4Valéria Veras de Paula5Alexsandra Fernandes Pereira6Arlindo Alencar Araripe Moura7Alexandre Rodrigues Silva8Universidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal Rural do Semi-ÁridoUniversidade Federal do CearáUniversidade Federal Rural do Semi-ÁridoThe study of the seminal plasma help us to understand the mechanisms by which reactive oxygen species (ROS) affect the sperm. The antioxidant enzymes, as the superoxide dismutase - SOD and catalase - CAT, are capable of removing the oxidative agents before they produce injuries. The aim of the current study was to investigate the activity of antioxidant enzymes SOD and CAT in seminal plasma, and their association with sperm quality in collared peccaries. Study was conducted during the dry period (August and September) on a region characterized by a semiarid climate, with an average annual temperature of 27°C and irregular rainfall (Mossoro, RN, Brazil; 5°10´S and 37°10´W). Nine ejaculates were obtained from sexually mature males (1 sample per animal) by electroejaculation. Semen was evaluated for microscopic parameters and the activity of SOD and CAT was measured by spectrophotometry. All ejaculates were white in color. Mean values for concentration were of 207 ± 160 x106 sperm/mL, motility of 83.0 ± 20.9% and viability of 72.5 ± 10.4%. In regards to the enzymatic activity, none was observed for the CAT enzyme. Trace levels of SOD (0.034 ± 0.049 AU/mgP) were detected in the ejaculates of all individuals; however, no correlation was observed between SOD levels and the sperm motility (R = 0.35; P = 0.931), vigor (R = 0.29; P = 0.133), viability (R = 0.16; P = 0.29), functional membrane (R = 0.04; P = 0.617) or morphology (R = 0.03; P = 0.637). In conclusion, we demonstrated the first description of antioxidant enzyme activity in seminal plasma of fresh ejaculates obtained from collared peccaries. SOD antioxidant activity was evident during the dry period of a semi-arid region, but no relationship between SOD and semen parameters was observed.http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/29761Oxidative stressSemenWild mammalsBiobank. |
| spellingShingle | Érika Aparecida Araújo dos Santos Patrícia Cunha Sousa Andreia Maria da Silva Ariana Lopes Correia Paiva Arthur Emmanuel de Araújo Lago Valéria Veras de Paula Alexsandra Fernandes Pereira Arlindo Alencar Araripe Moura Alexandre Rodrigues Silva Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality Semina: Ciências Agrárias Oxidative stress Semen Wild mammals Biobank. |
| title | Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality |
| title_full | Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality |
| title_fullStr | Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality |
| title_full_unstemmed | Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality |
| title_short | Superoxide dismutase and catalase activity in collared peccary (Pecari tajacu) seminal plasma and their relation to sperm quality |
| title_sort | superoxide dismutase and catalase activity in collared peccary pecari tajacu seminal plasma and their relation to sperm quality |
| topic | Oxidative stress Semen Wild mammals Biobank. |
| url | http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/29761 |
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