Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells
The morphological feature of apoptosis is induced by oxygen and glucose deprivation (OGD) in cardiomyocytes H9c2 cells. Salvianolic acid B (Sal-B) has been studied in several pathological progresses, whereas it is still unclear whether maternally expressed gene 3 (MEG3) is an intermediate regulator...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1621885 |
| _version_ | 1817981416269414400 |
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| author | Bin Yang Chunyan Zheng Haichu Yu Rui Zhang Cong Zhao Shanglang Cai |
| author_facet | Bin Yang Chunyan Zheng Haichu Yu Rui Zhang Cong Zhao Shanglang Cai |
| author_sort | Bin Yang |
| collection | DOAJ |
| description | The morphological feature of apoptosis is induced by oxygen and glucose deprivation (OGD) in cardiomyocytes H9c2 cells. Salvianolic acid B (Sal-B) has been studied in several pathological progresses, whereas it is still unclear whether maternally expressed gene 3 (MEG3) is an intermediate regulator during this progress. After pre-incubation with Sal-B and stimulation with OGD, viability and apoptosis of were examined in MEG3-overexpressed H9c2 cells. Cyclin D1, apoptosis-correlated proteins and regulators of signalling pathways were quantified with Western blot assay. MEG3 was detected by quantitative reverse transcription PCR (qRT-PCR). Sal-B was implicated in the enhancement of cell viability and suppression of apoptosis in OGD-treated H9c2 cells by repressing MEG3. In addition, MEG3 overexpression exerted an inhibitory effect on murine double minute 2 (MDM2) expression while aggrandized p53 expression in OGD-treated H9c2 cells which were pre-incubated with Sal-B. Furthermore, MEG3 overexpression abolished the up-regulative effect of Sal-B on phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) in OGD-treated H9c2 cells. These results indicated that cardio-protective function of Sal-B might be ascribed to its down-regulatory property on MEG3 expression which hence blocks p53 and triggers AMPK activation in OGD-treated cells. |
| first_indexed | 2024-04-13T23:05:40Z |
| format | Article |
| id | doaj.art-f2e0516b149b4339b89970665a6c9eb8 |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T23:05:40Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-f2e0516b149b4339b89970665a6c9eb82022-12-22T02:25:41ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014712274228110.1080/21691401.2019.1621885Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cellsBin Yang0Chunyan Zheng1Haichu Yu2Rui Zhang3Cong Zhao4Shanglang Cai5Department of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaMedical Consultation Center, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaDepartment of Cardiology, The Affiliated Hospital of Qingdao University, Qingdao, ChinaThe morphological feature of apoptosis is induced by oxygen and glucose deprivation (OGD) in cardiomyocytes H9c2 cells. Salvianolic acid B (Sal-B) has been studied in several pathological progresses, whereas it is still unclear whether maternally expressed gene 3 (MEG3) is an intermediate regulator during this progress. After pre-incubation with Sal-B and stimulation with OGD, viability and apoptosis of were examined in MEG3-overexpressed H9c2 cells. Cyclin D1, apoptosis-correlated proteins and regulators of signalling pathways were quantified with Western blot assay. MEG3 was detected by quantitative reverse transcription PCR (qRT-PCR). Sal-B was implicated in the enhancement of cell viability and suppression of apoptosis in OGD-treated H9c2 cells by repressing MEG3. In addition, MEG3 overexpression exerted an inhibitory effect on murine double minute 2 (MDM2) expression while aggrandized p53 expression in OGD-treated H9c2 cells which were pre-incubated with Sal-B. Furthermore, MEG3 overexpression abolished the up-regulative effect of Sal-B on phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) in OGD-treated H9c2 cells. These results indicated that cardio-protective function of Sal-B might be ascribed to its down-regulatory property on MEG3 expression which hence blocks p53 and triggers AMPK activation in OGD-treated cells.https://www.tandfonline.com/doi/10.1080/21691401.2019.1621885Salvianolic acid BMEG3MDM2/p53AMPK |
| spellingShingle | Bin Yang Chunyan Zheng Haichu Yu Rui Zhang Cong Zhao Shanglang Cai Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells Artificial Cells, Nanomedicine, and Biotechnology Salvianolic acid B MEG3 MDM2/p53 AMPK |
| title | Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells |
| title_full | Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells |
| title_fullStr | Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells |
| title_full_unstemmed | Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells |
| title_short | Cardio-protective effects of salvianolic acid B on oxygen and glucose deprivation (OGD)-treated H9c2 cells |
| title_sort | cardio protective effects of salvianolic acid b on oxygen and glucose deprivation ogd treated h9c2 cells |
| topic | Salvianolic acid B MEG3 MDM2/p53 AMPK |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1621885 |
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