Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability
<p>Abstract</p> <p>Nonsteroidal anti-inflammatory drugs (NSAIDs) have been proposed for prevention and treatment of a variety of human cancers. Piroxicam, in particular, has been recently shown to exert significant anti-tumoral activity in combination with cisplatin (CDDP) on mesot...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-05-01
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| Series: | Journal of Translational Medicine |
| Online Access: | http://www.translational-medicine.com/content/6/1/27 |
| _version_ | 1818021713941626880 |
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| author | Sacchi Ada Altucci Lucia Menegozzo Simona Galati Rossella Nebbioso Angela Cardillo Irene Verdina Alessandra Baldi Alfonso |
| author_facet | Sacchi Ada Altucci Lucia Menegozzo Simona Galati Rossella Nebbioso Angela Cardillo Irene Verdina Alessandra Baldi Alfonso |
| author_sort | Sacchi Ada |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Nonsteroidal anti-inflammatory drugs (NSAIDs) have been proposed for prevention and treatment of a variety of human cancers. Piroxicam, in particular, has been recently shown to exert significant anti-tumoral activity in combination with cisplatin (CDDP) on mesothelioma cells. However, the mechanisms through which NSAIDs regulate the cell cycle as well as the signal pathways involved in the growth inhibition, remain unclear. In the present study, using two mesothelioma cell lines, MSTO-211H and NCI-H2452, we have investigated the influence of piroxicam alone and in association with CDDP on proliferation, cell cycle regulation and apoptosis. In both cell lines a significant effect on cell growth inhibition, respect to the control, was observed with all the drugs tested. Moreover, treatment with piroxicam or CDDP alone altered the cell cycle phase distribution as well as the expression of some cell cycle regulatory proteins in both cell lines. These effects were increased, even if in a not completely overlapping manner, after treatment with the association of piroxicam and CDDP. In particular, the two drugs in NCI cell line had a synergistic effect on apoptosis, probably through activation of caspase 8 and caspase 9, while the most evident targets among the cell cycle regulators were cyclin D1 and p21<sup>waf1</sup>. These results suggest that the association of piroxicam and CDDP specifically triggers cell cycle regulation and apoptosis in different mesothelioma cell lines and may hold promise in the treatment of mesothelioma.</p> |
| first_indexed | 2024-04-14T08:21:31Z |
| format | Article |
| id | doaj.art-f2e1bb2152464b69919c95d3d996b97a |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-04-14T08:21:31Z |
| publishDate | 2008-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-f2e1bb2152464b69919c95d3d996b97a2022-12-22T02:04:11ZengBMCJournal of Translational Medicine1479-58762008-05-01612710.1186/1479-5876-6-27Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viabilitySacchi AdaAltucci LuciaMenegozzo SimonaGalati RossellaNebbioso AngelaCardillo IreneVerdina AlessandraBaldi Alfonso<p>Abstract</p> <p>Nonsteroidal anti-inflammatory drugs (NSAIDs) have been proposed for prevention and treatment of a variety of human cancers. Piroxicam, in particular, has been recently shown to exert significant anti-tumoral activity in combination with cisplatin (CDDP) on mesothelioma cells. However, the mechanisms through which NSAIDs regulate the cell cycle as well as the signal pathways involved in the growth inhibition, remain unclear. In the present study, using two mesothelioma cell lines, MSTO-211H and NCI-H2452, we have investigated the influence of piroxicam alone and in association with CDDP on proliferation, cell cycle regulation and apoptosis. In both cell lines a significant effect on cell growth inhibition, respect to the control, was observed with all the drugs tested. Moreover, treatment with piroxicam or CDDP alone altered the cell cycle phase distribution as well as the expression of some cell cycle regulatory proteins in both cell lines. These effects were increased, even if in a not completely overlapping manner, after treatment with the association of piroxicam and CDDP. In particular, the two drugs in NCI cell line had a synergistic effect on apoptosis, probably through activation of caspase 8 and caspase 9, while the most evident targets among the cell cycle regulators were cyclin D1 and p21<sup>waf1</sup>. These results suggest that the association of piroxicam and CDDP specifically triggers cell cycle regulation and apoptosis in different mesothelioma cell lines and may hold promise in the treatment of mesothelioma.</p>http://www.translational-medicine.com/content/6/1/27 |
| spellingShingle | Sacchi Ada Altucci Lucia Menegozzo Simona Galati Rossella Nebbioso Angela Cardillo Irene Verdina Alessandra Baldi Alfonso Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability Journal of Translational Medicine |
| title | Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability |
| title_full | Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability |
| title_fullStr | Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability |
| title_full_unstemmed | Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability |
| title_short | Molecular analysis of the effects of Piroxicam and Cisplatin on mesothelioma cells growth and viability |
| title_sort | molecular analysis of the effects of piroxicam and cisplatin on mesothelioma cells growth and viability |
| url | http://www.translational-medicine.com/content/6/1/27 |
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