Fungal Diversity in Barley Under Different Storage Conditions
The diversity of fungi in barley in simulated storage environments was analyzed. Barley was stored at different temperatures (15, 25, 35°C) and relative humidity (55, 65, 75, 85 RH) for 180 and 360 days. Alpha diversity, beta diversity, species composition, and species differences were analyzed usin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.895975/full |
| _version_ | 1828314970938408960 |
|---|---|
| author | Dongmei Cao Dongmei Cao Dongmei Cao Dongmei Cao Yuhao Lou Xiujie Jiang Xiujie Jiang Dongjie Zhang Dongjie Zhang Dongjie Zhang Dongjie Zhang Dongjie Zhang Junmei Liu |
| author_facet | Dongmei Cao Dongmei Cao Dongmei Cao Dongmei Cao Yuhao Lou Xiujie Jiang Xiujie Jiang Dongjie Zhang Dongjie Zhang Dongjie Zhang Dongjie Zhang Dongjie Zhang Junmei Liu |
| author_sort | Dongmei Cao |
| collection | DOAJ |
| description | The diversity of fungi in barley in simulated storage environments was analyzed. Barley was stored at different temperatures (15, 25, 35°C) and relative humidity (55, 65, 75, 85 RH) for 180 and 360 days. Alpha diversity, beta diversity, species composition, and species differences were analyzed using Illumina HiSeq technology. The fungal communities in all barley samples before and after storage belonged to 3 phyla, 18 classes, 39 orders, 71 families, 103 genera, and 152 species. The relative abundance of the dominant phylum Ascomycota was 77.98–99.19%. The relative abundance of Basidiomycota was 0.77–21.96%. At the genus level, the dominant genera of fungi in barley initially included Fusarium, Aspergillus, Microdochium, Alternaria, and Epicoccum. After 360 days of storage, the dominant genera became Epicoccum, Alternaria, Bipolar, Cladosporium, Fusarium, and Aspergillus. According to Venn diagrams and principal coordinates analysis, the fungal community diversity in barley initially was much higher than in barley stored at different temperatures and humidity. The application of PLS-DA could accurately distinguish between barley stored for 180 and 360 days. Some high-temperature and high-humidity environments accelerated storage. The dominant genera differed in different storage conditions and constantly changed with increasing storage duration. Epicoccum was one of the dominant genera after longer storage periods. This study provides theoretical support for optimizing safe storage conditions in barley. |
| first_indexed | 2024-04-13T16:53:36Z |
| format | Article |
| id | doaj.art-f2e6004df8a5462abc37870ee549bf9f |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T16:53:36Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f2e6004df8a5462abc37870ee549bf9f2022-12-22T02:38:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.895975895975Fungal Diversity in Barley Under Different Storage ConditionsDongmei Cao0Dongmei Cao1Dongmei Cao2Dongmei Cao3Yuhao Lou4Xiujie Jiang5Xiujie Jiang6Dongjie Zhang7Dongjie Zhang8Dongjie Zhang9Dongjie Zhang10Dongjie Zhang11Junmei Liu12College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang Province, Daqing, ChinaHeilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, ChinaCollege of Food Science, Heilongjiang Bayi Agricultural University, Daqing, ChinaNational Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing, ChinaKey Laboratory of Agro-Products Processing and Quality Safety of Heilongjiang Province, Daqing, ChinaHeilongjiang Engineering Research Center for Coarse Cereals Processing and Quality Safety, Daqing, ChinaHeilongjiang Province Cultivating Collaborative Innovation Center for the Beidahuang Modern Agricultural Industry Technology, Daqing, ChinaCollege of Food Science, Jilin Agricultural University, Daqing, ChinaThe diversity of fungi in barley in simulated storage environments was analyzed. Barley was stored at different temperatures (15, 25, 35°C) and relative humidity (55, 65, 75, 85 RH) for 180 and 360 days. Alpha diversity, beta diversity, species composition, and species differences were analyzed using Illumina HiSeq technology. The fungal communities in all barley samples before and after storage belonged to 3 phyla, 18 classes, 39 orders, 71 families, 103 genera, and 152 species. The relative abundance of the dominant phylum Ascomycota was 77.98–99.19%. The relative abundance of Basidiomycota was 0.77–21.96%. At the genus level, the dominant genera of fungi in barley initially included Fusarium, Aspergillus, Microdochium, Alternaria, and Epicoccum. After 360 days of storage, the dominant genera became Epicoccum, Alternaria, Bipolar, Cladosporium, Fusarium, and Aspergillus. According to Venn diagrams and principal coordinates analysis, the fungal community diversity in barley initially was much higher than in barley stored at different temperatures and humidity. The application of PLS-DA could accurately distinguish between barley stored for 180 and 360 days. Some high-temperature and high-humidity environments accelerated storage. The dominant genera differed in different storage conditions and constantly changed with increasing storage duration. Epicoccum was one of the dominant genera after longer storage periods. This study provides theoretical support for optimizing safe storage conditions in barley.https://www.frontiersin.org/articles/10.3389/fmicb.2022.895975/fullbarleyhigh-throughput sequencing technologyfungal diversitydifferent storage environmentdeoxynivalenol |
| spellingShingle | Dongmei Cao Dongmei Cao Dongmei Cao Dongmei Cao Yuhao Lou Xiujie Jiang Xiujie Jiang Dongjie Zhang Dongjie Zhang Dongjie Zhang Dongjie Zhang Dongjie Zhang Junmei Liu Fungal Diversity in Barley Under Different Storage Conditions Frontiers in Microbiology barley high-throughput sequencing technology fungal diversity different storage environment deoxynivalenol |
| title | Fungal Diversity in Barley Under Different Storage Conditions |
| title_full | Fungal Diversity in Barley Under Different Storage Conditions |
| title_fullStr | Fungal Diversity in Barley Under Different Storage Conditions |
| title_full_unstemmed | Fungal Diversity in Barley Under Different Storage Conditions |
| title_short | Fungal Diversity in Barley Under Different Storage Conditions |
| title_sort | fungal diversity in barley under different storage conditions |
| topic | barley high-throughput sequencing technology fungal diversity different storage environment deoxynivalenol |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.895975/full |
| work_keys_str_mv | AT dongmeicao fungaldiversityinbarleyunderdifferentstorageconditions AT dongmeicao fungaldiversityinbarleyunderdifferentstorageconditions AT dongmeicao fungaldiversityinbarleyunderdifferentstorageconditions AT dongmeicao fungaldiversityinbarleyunderdifferentstorageconditions AT yuhaolou fungaldiversityinbarleyunderdifferentstorageconditions AT xiujiejiang fungaldiversityinbarleyunderdifferentstorageconditions AT xiujiejiang fungaldiversityinbarleyunderdifferentstorageconditions AT dongjiezhang fungaldiversityinbarleyunderdifferentstorageconditions AT dongjiezhang fungaldiversityinbarleyunderdifferentstorageconditions AT dongjiezhang fungaldiversityinbarleyunderdifferentstorageconditions AT dongjiezhang fungaldiversityinbarleyunderdifferentstorageconditions AT dongjiezhang fungaldiversityinbarleyunderdifferentstorageconditions AT junmeiliu fungaldiversityinbarleyunderdifferentstorageconditions |