Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying
Induced molting is a common method to obtain a new life in laying hens, in which periodic changes in feathers are the prominent feature. Nevertheless, its precise molecular mechanism remains unclear. In this study, feather and hair follicle samples were collected during fasting-induced physiological...
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MDPI AG
2023-10-01
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| author | Lujie Zhang Chunxia Cai Xinxin Liu Xiaoran Zhang Zhiyuan An Enyou Zhou Jianzeng Li Zhuanjian Li Wenting Li Guirong Sun Guoxi Li Xiangtao Kang Ruili Han Ruirui Jiang |
| author_facet | Lujie Zhang Chunxia Cai Xinxin Liu Xiaoran Zhang Zhiyuan An Enyou Zhou Jianzeng Li Zhuanjian Li Wenting Li Guirong Sun Guoxi Li Xiangtao Kang Ruili Han Ruirui Jiang |
| author_sort | Lujie Zhang |
| collection | DOAJ |
| description | Induced molting is a common method to obtain a new life in laying hens, in which periodic changes in feathers are the prominent feature. Nevertheless, its precise molecular mechanism remains unclear. In this study, feather and hair follicle samples were collected during fasting-induced physiological remodeling for hematoxylin–eosin staining, hormone changes and follicle traits, and transcriptome sequencing. Feather shedding was observed in F13 to R25, while newborns were observed in R3 to R32. Triiodothyronine and tetraiodothyronine were significantly elevated during feather shedding. The calcium content was significantly higher, and the ash content was significantly lower after the changeover. The determination of hair follicle traits revealed an increasing trend in pore density and a decrease in pore diameter after the resumption of feeding. According to RNA-seq results, several core genes were identified, including <i>DSP</i>, <i>CDH1</i>, <i>PKP1</i>, and <i>PPCKB</i>, which may have an impact on hair follicle growth. The focus was to discover that starvation may trigger changes in thyroid hormones, which in turn regulate feather molting through thyroid hormone synthesis, calcium signaling, and thyroid hormone signaling pathways. These data provide a valuable resource for the analysis of the molecular mechanisms underlying the cyclical growth of hair follicles in the skin during induced molting. |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T21:26:00Z |
| publishDate | 2023-10-01 |
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| spelling | doaj.art-f2e84c5a42774b3abfcfa5829ef12f032023-11-19T15:44:00ZengMDPI AGBiology2079-77372023-10-011210134510.3390/biology12101345Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg LayingLujie Zhang0Chunxia Cai1Xinxin Liu2Xiaoran Zhang3Zhiyuan An4Enyou Zhou5Jianzeng Li6Zhuanjian Li7Wenting Li8Guirong Sun9Guoxi Li10Xiangtao Kang11Ruili Han12Ruirui Jiang13The Shennong Laboratory, Zhengzhou 450002, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaThe Shennong Laboratory, Zhengzhou 450002, ChinaCollege of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, ChinaInduced molting is a common method to obtain a new life in laying hens, in which periodic changes in feathers are the prominent feature. Nevertheless, its precise molecular mechanism remains unclear. In this study, feather and hair follicle samples were collected during fasting-induced physiological remodeling for hematoxylin–eosin staining, hormone changes and follicle traits, and transcriptome sequencing. Feather shedding was observed in F13 to R25, while newborns were observed in R3 to R32. Triiodothyronine and tetraiodothyronine were significantly elevated during feather shedding. The calcium content was significantly higher, and the ash content was significantly lower after the changeover. The determination of hair follicle traits revealed an increasing trend in pore density and a decrease in pore diameter after the resumption of feeding. According to RNA-seq results, several core genes were identified, including <i>DSP</i>, <i>CDH1</i>, <i>PKP1</i>, and <i>PPCKB</i>, which may have an impact on hair follicle growth. The focus was to discover that starvation may trigger changes in thyroid hormones, which in turn regulate feather molting through thyroid hormone synthesis, calcium signaling, and thyroid hormone signaling pathways. These data provide a valuable resource for the analysis of the molecular mechanisms underlying the cyclical growth of hair follicles in the skin during induced molting.https://www.mdpi.com/2079-7737/12/10/1345induced moltingfeathershair folliclesregenerationtranscriptomethyroid hormones |
| spellingShingle | Lujie Zhang Chunxia Cai Xinxin Liu Xiaoran Zhang Zhiyuan An Enyou Zhou Jianzeng Li Zhuanjian Li Wenting Li Guirong Sun Guoxi Li Xiangtao Kang Ruili Han Ruirui Jiang Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying Biology induced molting feathers hair follicles regeneration transcriptome thyroid hormones |
| title | Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying |
| title_full | Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying |
| title_fullStr | Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying |
| title_full_unstemmed | Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying |
| title_short | Multi-Stage Transcriptome Analysis Revealed the Growth Mechanism of Feathers and Hair Follicles during Induction Molting by Fasting in the Late Stage of Egg Laying |
| title_sort | multi stage transcriptome analysis revealed the growth mechanism of feathers and hair follicles during induction molting by fasting in the late stage of egg laying |
| topic | induced molting feathers hair follicles regeneration transcriptome thyroid hormones |
| url | https://www.mdpi.com/2079-7737/12/10/1345 |
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