| Summary: | <i>Ralstonia solanacearum</i> is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of <i>R. solanacearum</i> can act as a biocontrol mediator for managing potato bacterial wilt. Virulent <i>R. solanacearum</i> was isolated and identified (GenBank accession number; OP180100). The avirulent strain was obtained from the virulent strain through storage for 3 weeks until the development of deep red colonies. The virulent strain had higher lytic activity than the avirulent strain. Tubers’ treatments by the avirulent strain of <i>R. solanacearum</i>, (supernatant, boiled supernatant, and dead cells) significantly reduced plant disease rating and increased the growth, physiological activities, and biomass of potato compared to the untreated, infected control. The major components detected by GC–MS in the supernatant revealed 10.86% palmitic acid (virulent), and 18.03% 1,3-dioxolane, 2,4,5-trimethyl- (avirulent), whereas the major component in the boiled supernatant was 2-hydroxy-gamma-butyrolactone in the virulent (21.17%) and avirulent (27.78%) strains. This is the first research that assessed the influence of boiled supernatant and dead cells of virulent and avirulent <i>R.</i><i>solanacearum</i> strains in controlling bacterial wilt disease. Additional work is encouraged for further elucidation of such a topic.
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