New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i>
<i>Ralstonia solanacearum</i> is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of <i>R. solanacearum</i> can act as a biocontrol mediato...
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1814 |
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| author | Zeiad Moussa Ehsan M. Rashad Elsherbiny A. Elsherbiny Abdulaziz A. Al-Askar Amr Abker Arishi Fatimah O. Al-Otibi WesamEldin I. A. Saber |
| author_facet | Zeiad Moussa Ehsan M. Rashad Elsherbiny A. Elsherbiny Abdulaziz A. Al-Askar Amr Abker Arishi Fatimah O. Al-Otibi WesamEldin I. A. Saber |
| author_sort | Zeiad Moussa |
| collection | DOAJ |
| description | <i>Ralstonia solanacearum</i> is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of <i>R. solanacearum</i> can act as a biocontrol mediator for managing potato bacterial wilt. Virulent <i>R. solanacearum</i> was isolated and identified (GenBank accession number; OP180100). The avirulent strain was obtained from the virulent strain through storage for 3 weeks until the development of deep red colonies. The virulent strain had higher lytic activity than the avirulent strain. Tubers’ treatments by the avirulent strain of <i>R. solanacearum</i>, (supernatant, boiled supernatant, and dead cells) significantly reduced plant disease rating and increased the growth, physiological activities, and biomass of potato compared to the untreated, infected control. The major components detected by GC–MS in the supernatant revealed 10.86% palmitic acid (virulent), and 18.03% 1,3-dioxolane, 2,4,5-trimethyl- (avirulent), whereas the major component in the boiled supernatant was 2-hydroxy-gamma-butyrolactone in the virulent (21.17%) and avirulent (27.78%) strains. This is the first research that assessed the influence of boiled supernatant and dead cells of virulent and avirulent <i>R.</i><i>solanacearum</i> strains in controlling bacterial wilt disease. Additional work is encouraged for further elucidation of such a topic. |
| first_indexed | 2024-03-09T23:06:24Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T23:06:24Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f2e9e9b8c53a4466a5efe4cc35674add2023-11-23T17:53:40ZengMDPI AGMicroorganisms2076-26072022-09-01109181410.3390/microorganisms10091814New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i>Zeiad Moussa0Ehsan M. Rashad1Elsherbiny A. Elsherbiny2Abdulaziz A. Al-Askar3Amr Abker Arishi4Fatimah O. Al-Otibi5WesamEldin I. A. Saber6Microbial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, EgyptDepartment of Seed Pathology Research, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, EgyptPlant Pathology Department, Faculty of Agriculture, Mansoura University, Mansoura 35516, EgyptDepartment of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh 11451, Saudi ArabiaSchool of Molecular Sciences, The University of Western Australia, Perth, WA 6009, AustraliaDepartment of Botany and Microbiology, Faculty of Science, King Saud University, Riyadh 11451, Saudi ArabiaMicrobial Activity Unit, Microbiology Department, Soils, Water and Environment Research Institute, Agricultural Research Center, Giza 12619, Egypt<i>Ralstonia solanacearum</i> is one of the globally significant plant pathogens that infect a wide host range of economically important plants. A study was conducted to evaluate the hypothesis that an avirulent strain of <i>R. solanacearum</i> can act as a biocontrol mediator for managing potato bacterial wilt. Virulent <i>R. solanacearum</i> was isolated and identified (GenBank accession number; OP180100). The avirulent strain was obtained from the virulent strain through storage for 3 weeks until the development of deep red colonies. The virulent strain had higher lytic activity than the avirulent strain. Tubers’ treatments by the avirulent strain of <i>R. solanacearum</i>, (supernatant, boiled supernatant, and dead cells) significantly reduced plant disease rating and increased the growth, physiological activities, and biomass of potato compared to the untreated, infected control. The major components detected by GC–MS in the supernatant revealed 10.86% palmitic acid (virulent), and 18.03% 1,3-dioxolane, 2,4,5-trimethyl- (avirulent), whereas the major component in the boiled supernatant was 2-hydroxy-gamma-butyrolactone in the virulent (21.17%) and avirulent (27.78%) strains. This is the first research that assessed the influence of boiled supernatant and dead cells of virulent and avirulent <i>R.</i><i>solanacearum</i> strains in controlling bacterial wilt disease. Additional work is encouraged for further elucidation of such a topic.https://www.mdpi.com/2076-2607/10/9/1814biological controlbacterial supernatantdefense-related enzymesGC–MS analysislytic enzymes |
| spellingShingle | Zeiad Moussa Ehsan M. Rashad Elsherbiny A. Elsherbiny Abdulaziz A. Al-Askar Amr Abker Arishi Fatimah O. Al-Otibi WesamEldin I. A. Saber New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i> Microorganisms biological control bacterial supernatant defense-related enzymes GC–MS analysis lytic enzymes |
| title | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i> |
| title_full | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i> |
| title_fullStr | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i> |
| title_full_unstemmed | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i> |
| title_short | New Strategy for Inducing Resistance against Bacterial Wilt Disease Using an Avirulent Strain of <i>Ralstonia solanacearum</i> |
| title_sort | new strategy for inducing resistance against bacterial wilt disease using an avirulent strain of i ralstonia solanacearum i |
| topic | biological control bacterial supernatant defense-related enzymes GC–MS analysis lytic enzymes |
| url | https://www.mdpi.com/2076-2607/10/9/1814 |
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