Tissue Specific Labeling in Proteomics
Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in multicellular organisms and tissues poses a significant...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-07-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/5/3/17 |
| _version_ | 1798002533381177344 |
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| author | Evelyn Ramberger Gunnar Dittmar |
| author_facet | Evelyn Ramberger Gunnar Dittmar |
| author_sort | Evelyn Ramberger |
| collection | DOAJ |
| description | Mass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in multicellular organisms and tissues poses a significant challenge. Isolating a subset of cells from tissues requires mechanical and biochemical separation or sorting, a process which can alter cellular signaling, and thus, the composition of the proteome. Recently, several approaches for cell selective labeling of proteins, that include bioorthogonal amino acids, biotinylating enzymes, and genetic tools, have been developed. These tools facilitate the selective labeling of proteins, their interactome, or of specific cell types within a tissue or an organism, while avoiding the difficult and contamination-prone biochemical separation of cells from the tissue. In this review, we give an overview of existing techniques and their application in cell culture models and whole animals. |
| first_indexed | 2024-04-11T11:53:40Z |
| format | Article |
| id | doaj.art-f2ef811b0df649dd8d5b1955c68aa7d3 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-11T11:53:40Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-f2ef811b0df649dd8d5b1955c68aa7d32022-12-22T04:25:14ZengMDPI AGProteomes2227-73822017-07-01531710.3390/proteomes5030017proteomes5030017Tissue Specific Labeling in ProteomicsEvelyn Ramberger0Gunnar Dittmar1Mass-Spectrometry Core Unit, Max Delbrück Center for Molecular Medicine, 13125 Berlin, GermanyProteome and Genome Research Laboratory, Luxembourg Institute of Health, 1272 Strassen, LuxembourgMass spectrometry-based proteomics is a powerful tool for identifying and quantifying proteins in biological samples. While it is routinely used for the characterization of simple cell line systems, the analysis of the cell specific proteome in multicellular organisms and tissues poses a significant challenge. Isolating a subset of cells from tissues requires mechanical and biochemical separation or sorting, a process which can alter cellular signaling, and thus, the composition of the proteome. Recently, several approaches for cell selective labeling of proteins, that include bioorthogonal amino acids, biotinylating enzymes, and genetic tools, have been developed. These tools facilitate the selective labeling of proteins, their interactome, or of specific cell types within a tissue or an organism, while avoiding the difficult and contamination-prone biochemical separation of cells from the tissue. In this review, we give an overview of existing techniques and their application in cell culture models and whole animals.https://www.mdpi.com/2227-7382/5/3/17quantitative proteomicsstable isotope labelingtissueamino acid analogbiotinylationbioorthogonalmulticellularSILACAPEXBioID |
| spellingShingle | Evelyn Ramberger Gunnar Dittmar Tissue Specific Labeling in Proteomics Proteomes quantitative proteomics stable isotope labeling tissue amino acid analog biotinylation bioorthogonal multicellular SILAC APEX BioID |
| title | Tissue Specific Labeling in Proteomics |
| title_full | Tissue Specific Labeling in Proteomics |
| title_fullStr | Tissue Specific Labeling in Proteomics |
| title_full_unstemmed | Tissue Specific Labeling in Proteomics |
| title_short | Tissue Specific Labeling in Proteomics |
| title_sort | tissue specific labeling in proteomics |
| topic | quantitative proteomics stable isotope labeling tissue amino acid analog biotinylation bioorthogonal multicellular SILAC APEX BioID |
| url | https://www.mdpi.com/2227-7382/5/3/17 |
| work_keys_str_mv | AT evelynramberger tissuespecificlabelinginproteomics AT gunnardittmar tissuespecificlabelinginproteomics |