Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails
Despite their impressive diversity and already broad therapeutic applications, cone snail venoms have received less attention as a natural source in the investigation of antimicrobial peptides than other venomous animals such as scorpions, spiders, or snakes. Cone snails are among the largest genera...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/9/4/344 |
| _version_ | 1827696532053295104 |
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| author | Anicet Ebou Dominique Koua Audrey Addablah Solange Kakou-Ngazoa Sébastien Dutertre |
| author_facet | Anicet Ebou Dominique Koua Audrey Addablah Solange Kakou-Ngazoa Sébastien Dutertre |
| author_sort | Anicet Ebou |
| collection | DOAJ |
| description | Despite their impressive diversity and already broad therapeutic applications, cone snail venoms have received less attention as a natural source in the investigation of antimicrobial peptides than other venomous animals such as scorpions, spiders, or snakes. Cone snails are among the largest genera (<i>Conus</i> sp.) of marine invertebrates, with more than seven hundred species described to date. These predatory mollusks use their sophisticated venom apparatus to capture prey or defend themselves. In-depth studies of these venoms have unraveled many biologically active peptides with pharmacological properties of interest in the field of pain management, the treatment of epilepsy, neurodegenerative diseases, and cardiac ischemia. Considering sequencing efficiency and affordability, cone snail venom gland transcriptome analyses could allow the discovery of new, promising antimicrobial peptides. We first present here the need for novel compounds like antimicrobial peptides as a viable alternative to conventional antibiotics. Secondly, we review the current knowledge on cone snails as a source of antimicrobial peptides. Then, we present the current state of the art in analytical methods applied to crude or milked venom followed by how antibacterial activity assay can be implemented for fostering cone snail antimicrobial peptides studies. We also propose a new innovative profile Hidden Markov model-based approach to annotate full venom gland transcriptomes and speed up the discovery of potentially active peptides from cone snails. |
| first_indexed | 2024-03-10T12:48:55Z |
| format | Article |
| id | doaj.art-f2ef880302f04fa5850088de091cb974 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T12:48:55Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-f2ef880302f04fa5850088de091cb9742023-11-21T13:17:56ZengMDPI AGBiomedicines2227-90592021-03-019434410.3390/biomedicines9040344Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone SnailsAnicet Ebou0Dominique Koua1Audrey Addablah2Solange Kakou-Ngazoa3Sébastien Dutertre4Bioinformatic Team, Département Agriculture et Ressource Animales, UMRI 28, Institut National Polytechnique Félix Houphouët-Boigny, Yamoussoukro BP 1093, Ivory CoastBioinformatic Team, Département Agriculture et Ressource Animales, UMRI 28, Institut National Polytechnique Félix Houphouët-Boigny, Yamoussoukro BP 1093, Ivory CoastPlateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, Abidjan BP 490, Ivory CoastPlateforme de Biologie Moléculaire, Institut Pasteur de Côte d’Ivoire, Abidjan BP 490, Ivory CoastInstitut des Biomolécules Max Mousseron, UMR 5247, Université de Montpellier, CNRS, ENSCM, 34095 Montpellier, FranceDespite their impressive diversity and already broad therapeutic applications, cone snail venoms have received less attention as a natural source in the investigation of antimicrobial peptides than other venomous animals such as scorpions, spiders, or snakes. Cone snails are among the largest genera (<i>Conus</i> sp.) of marine invertebrates, with more than seven hundred species described to date. These predatory mollusks use their sophisticated venom apparatus to capture prey or defend themselves. In-depth studies of these venoms have unraveled many biologically active peptides with pharmacological properties of interest in the field of pain management, the treatment of epilepsy, neurodegenerative diseases, and cardiac ischemia. Considering sequencing efficiency and affordability, cone snail venom gland transcriptome analyses could allow the discovery of new, promising antimicrobial peptides. We first present here the need for novel compounds like antimicrobial peptides as a viable alternative to conventional antibiotics. Secondly, we review the current knowledge on cone snails as a source of antimicrobial peptides. Then, we present the current state of the art in analytical methods applied to crude or milked venom followed by how antibacterial activity assay can be implemented for fostering cone snail antimicrobial peptides studies. We also propose a new innovative profile Hidden Markov model-based approach to annotate full venom gland transcriptomes and speed up the discovery of potentially active peptides from cone snails.https://www.mdpi.com/2227-9059/9/4/344cone snailsvenomconotoxinsantimicrobial peptidesantibacterial activityproteotranscriptomic approach |
| spellingShingle | Anicet Ebou Dominique Koua Audrey Addablah Solange Kakou-Ngazoa Sébastien Dutertre Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails Biomedicines cone snails venom conotoxins antimicrobial peptides antibacterial activity proteotranscriptomic approach |
| title | Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails |
| title_full | Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails |
| title_fullStr | Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails |
| title_full_unstemmed | Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails |
| title_short | Combined Proteotranscriptomic-Based Strategy to Discover Novel Antimicrobial Peptides from Cone Snails |
| title_sort | combined proteotranscriptomic based strategy to discover novel antimicrobial peptides from cone snails |
| topic | cone snails venom conotoxins antimicrobial peptides antibacterial activity proteotranscriptomic approach |
| url | https://www.mdpi.com/2227-9059/9/4/344 |
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