Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient
In the south of western Siberia (Russia), there are many unique and unexplored soda, saline, and freshwater lakes. In this study, the results are presented on microbial diversity, its metabolic potential, and their relation with a set of geochemical parameters for a hypersaline lake ecosystem in the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2079-7737/11/4/605 |
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| author | Alla V. Bryanskaya Aleksandra A. Shipova Alexei S. Rozanov Oxana A. Kolpakova Elena V. Lazareva Yulia E. Uvarova Vadim M. Efimov Sergey M. Zhmodik Oxana P. Taran Tatyana N. Goryachkovskaya Sergey E. Peltek |
| author_facet | Alla V. Bryanskaya Aleksandra A. Shipova Alexei S. Rozanov Oxana A. Kolpakova Elena V. Lazareva Yulia E. Uvarova Vadim M. Efimov Sergey M. Zhmodik Oxana P. Taran Tatyana N. Goryachkovskaya Sergey E. Peltek |
| author_sort | Alla V. Bryanskaya |
| collection | DOAJ |
| description | In the south of western Siberia (Russia), there are many unique and unexplored soda, saline, and freshwater lakes. In this study, the results are presented on microbial diversity, its metabolic potential, and their relation with a set of geochemical parameters for a hypersaline lake ecosystem in the Novosibirsk region (Oblast). The metagenomic approach used in this work allowed us to determine the composition and structure of a floating microbial community, the upper layer of silt, and the strata of bottom sediments in a natural saline lake via two bioinformatic approaches, whose results are in good agreement with each other. In the floating microbial community and in the upper layers of the bottom sediment, bacteria of the Proteobacteria (Gammaproteobacteria), Cyanobacteria, and Bacteroidetes phyla were found to predominate. The lower layers were dominated by Proteobacteria (mainly Deltaproteobacteria), Gemmatimonadetes, Firmicutes, and Archaea. Metabolic pathways were reconstructed to investigate the metabolic potential of the microbial communities and other hypothetical roles of the microbial communities in the biogeochemical cycle. Relations between different taxa of microorganisms were identified, as was their potential role in biogeochemical transformations of C, N, and S in a comparative structural analysis that included various ecological niches. |
| first_indexed | 2024-03-09T11:08:39Z |
| format | Article |
| id | doaj.art-f2ef9406647c461c878eca72242bbc48 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T11:08:39Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-f2ef9406647c461c878eca72242bbc482023-12-01T00:52:14ZengMDPI AGBiology2079-77372022-04-0111460510.3390/biology11040605Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical GradientAlla V. Bryanskaya0Aleksandra A. Shipova1Alexei S. Rozanov2Oxana A. Kolpakova3Elena V. Lazareva4Yulia E. Uvarova5Vadim M. Efimov6Sergey M. Zhmodik7Oxana P. Taran8Tatyana N. Goryachkovskaya9Sergey E. Peltek10Laboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaV.S. Sobolev Institute of Geology and Mineralogy SB RAS, 630090 Novosibirsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaV.S. Sobolev Institute of Geology and Mineralogy SB RAS, 630090 Novosibirsk, RussiaFRC Krasnoyarsk Science Center SB RAS, Institute of Chemistry and Chemical Technology SB RAS, 660036 Krasnoyarsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaLaboratory of Molecular Biotechnologies, Federal Research Center Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, RussiaIn the south of western Siberia (Russia), there are many unique and unexplored soda, saline, and freshwater lakes. In this study, the results are presented on microbial diversity, its metabolic potential, and their relation with a set of geochemical parameters for a hypersaline lake ecosystem in the Novosibirsk region (Oblast). The metagenomic approach used in this work allowed us to determine the composition and structure of a floating microbial community, the upper layer of silt, and the strata of bottom sediments in a natural saline lake via two bioinformatic approaches, whose results are in good agreement with each other. In the floating microbial community and in the upper layers of the bottom sediment, bacteria of the Proteobacteria (Gammaproteobacteria), Cyanobacteria, and Bacteroidetes phyla were found to predominate. The lower layers were dominated by Proteobacteria (mainly Deltaproteobacteria), Gemmatimonadetes, Firmicutes, and Archaea. Metabolic pathways were reconstructed to investigate the metabolic potential of the microbial communities and other hypothetical roles of the microbial communities in the biogeochemical cycle. Relations between different taxa of microorganisms were identified, as was their potential role in biogeochemical transformations of C, N, and S in a comparative structural analysis that included various ecological niches.https://www.mdpi.com/2079-7737/11/4/605metagenomesaline lakemicrobial communitymetabolic pathwaybiogeochemical cycle |
| spellingShingle | Alla V. Bryanskaya Aleksandra A. Shipova Alexei S. Rozanov Oxana A. Kolpakova Elena V. Lazareva Yulia E. Uvarova Vadim M. Efimov Sergey M. Zhmodik Oxana P. Taran Tatyana N. Goryachkovskaya Sergey E. Peltek Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient Biology metagenome saline lake microbial community metabolic pathway biogeochemical cycle |
| title | Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient |
| title_full | Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient |
| title_fullStr | Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient |
| title_full_unstemmed | Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient |
| title_short | Diversity and Metabolism of Microbial Communities in a Hypersaline Lake along a Geochemical Gradient |
| title_sort | diversity and metabolism of microbial communities in a hypersaline lake along a geochemical gradient |
| topic | metagenome saline lake microbial community metabolic pathway biogeochemical cycle |
| url | https://www.mdpi.com/2079-7737/11/4/605 |
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