Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens
In aged animals, the physiological functions of the gastrointestinal tract (GIT) are reduced. Dietary intervention is necessary to re-activate GIT functions. The objective of this study was to investigate the impacts of dietary combination of quercetin (Q) and vitamin E (VE) on the intestinal struct...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.860889/full |
| _version_ | 1818283519712952320 |
|---|---|
| author | Felix Kwame Amevor Zhifu Cui Xiaxia Du Zifan Ning Xun Deng Dan Xu Gang Shu Youhao Wu Xueqing Cao Wei Shuo Yaofu Tian Diyan Li Yan Wang Yao Zhang Xiaohui Du Qing Zhu Xue Han Xiaoling Zhao |
| author_facet | Felix Kwame Amevor Zhifu Cui Xiaxia Du Zifan Ning Xun Deng Dan Xu Gang Shu Youhao Wu Xueqing Cao Wei Shuo Yaofu Tian Diyan Li Yan Wang Yao Zhang Xiaohui Du Qing Zhu Xue Han Xiaoling Zhao |
| author_sort | Felix Kwame Amevor |
| collection | DOAJ |
| description | In aged animals, the physiological functions of the gastrointestinal tract (GIT) are reduced. Dietary intervention is necessary to re-activate GIT functions. The objective of this study was to investigate the impacts of dietary combination of quercetin (Q) and vitamin E (VE) on the intestinal structure and barrier integrity in aged breeder chickens. A sum of 400 (65-wks-old) Tianfu breeder hens were randomly allotted into four (4) groups with four (4) replicates, and fed with basal diet; basal diet supplemented with 0.4g/kg of Q; basal diet supplemented with 0.2g/kg of VE; and basal diet supplemented with the combination of Q (0.4 g/kg) and VE (0.2 g/kg) for 14 weeks. At the end of the 14th week, serum and gut segments were collected from eight hens per group for analyses. The results showed that Q+VE exerted synergistic effects on intestinal morphology by promoting villi height and crypt depth (P < 0.05), as well as mitigated the intestinal inflammatory damage of the aged hens, but decreased the concentration of serum D-lactate and diamine oxidase; and increased the levels of secretory immunoglobulin A (sIgA) and Mucin-2 mRNA (P < 0.05). Furthermore, the mRNA expression of intestinal tight junction proteins including occludin, ZO1, and claudin-1 was increased by Q+VE (P < 0.05). Moreover, Q+VE decreased the mRNA expression of the pro-inflammatory genes (TNF-α, IL-6, and IL-1β), and increased the expression of anti-inflammatory genes (IL-10 and IL-4) (P < 0.05). These results were consistent with the mRNA expression of Bax and Bcl-2. In addition, Q+VE protected the small intestinal tract from oxidative damage by increasing the levels of superoxide dismutase, total antioxidant capacity, glutathione peroxidase, catalase (P < 0.05), and the mRNA expression of SOD1 and GPx-2. However, Q+VE decreased malondialdehyde levels in the intestine compared to the control (P < 0.05). These results indicated that dietary Q+VE improved intestinal function in aged breeder hens, by protecting the intestinal structure and integrity. Therefore, Q+VE could act as an anti-aging agent to elevate the physiological functions of the small intestine in chickens. |
| first_indexed | 2024-12-13T00:38:12Z |
| format | Article |
| id | doaj.art-f2f4bcbc21ab433ea2866eb13f0c243e |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T00:38:12Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-f2f4bcbc21ab433ea2866eb13f0c243e2022-12-22T00:05:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-03-011310.3389/fimmu.2022.860889860889Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder HensFelix Kwame Amevor0Zhifu Cui1Xiaxia Du2Zifan Ning3Xun Deng4Dan Xu5Gang Shu6Youhao Wu7Xueqing Cao8Wei Shuo9Yaofu Tian10Diyan Li11Yan Wang12Yao Zhang13Xiaohui Du14Qing Zhu15Xue Han16Xiaoling Zhao17Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaDepartment of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaGuizhou Institute of Animal Husbandry and Veterinary Medicine, Guiyang, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaIn aged animals, the physiological functions of the gastrointestinal tract (GIT) are reduced. Dietary intervention is necessary to re-activate GIT functions. The objective of this study was to investigate the impacts of dietary combination of quercetin (Q) and vitamin E (VE) on the intestinal structure and barrier integrity in aged breeder chickens. A sum of 400 (65-wks-old) Tianfu breeder hens were randomly allotted into four (4) groups with four (4) replicates, and fed with basal diet; basal diet supplemented with 0.4g/kg of Q; basal diet supplemented with 0.2g/kg of VE; and basal diet supplemented with the combination of Q (0.4 g/kg) and VE (0.2 g/kg) for 14 weeks. At the end of the 14th week, serum and gut segments were collected from eight hens per group for analyses. The results showed that Q+VE exerted synergistic effects on intestinal morphology by promoting villi height and crypt depth (P < 0.05), as well as mitigated the intestinal inflammatory damage of the aged hens, but decreased the concentration of serum D-lactate and diamine oxidase; and increased the levels of secretory immunoglobulin A (sIgA) and Mucin-2 mRNA (P < 0.05). Furthermore, the mRNA expression of intestinal tight junction proteins including occludin, ZO1, and claudin-1 was increased by Q+VE (P < 0.05). Moreover, Q+VE decreased the mRNA expression of the pro-inflammatory genes (TNF-α, IL-6, and IL-1β), and increased the expression of anti-inflammatory genes (IL-10 and IL-4) (P < 0.05). These results were consistent with the mRNA expression of Bax and Bcl-2. In addition, Q+VE protected the small intestinal tract from oxidative damage by increasing the levels of superoxide dismutase, total antioxidant capacity, glutathione peroxidase, catalase (P < 0.05), and the mRNA expression of SOD1 and GPx-2. However, Q+VE decreased malondialdehyde levels in the intestine compared to the control (P < 0.05). These results indicated that dietary Q+VE improved intestinal function in aged breeder hens, by protecting the intestinal structure and integrity. Therefore, Q+VE could act as an anti-aging agent to elevate the physiological functions of the small intestine in chickens.https://www.frontiersin.org/articles/10.3389/fimmu.2022.860889/fullchickenantioxidantsintestinal immunityinflammationoxidative stress |
| spellingShingle | Felix Kwame Amevor Zhifu Cui Xiaxia Du Zifan Ning Xun Deng Dan Xu Gang Shu Youhao Wu Xueqing Cao Wei Shuo Yaofu Tian Diyan Li Yan Wang Yao Zhang Xiaohui Du Qing Zhu Xue Han Xiaoling Zhao Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens Frontiers in Immunology chicken antioxidants intestinal immunity inflammation oxidative stress |
| title | Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens |
| title_full | Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens |
| title_fullStr | Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens |
| title_full_unstemmed | Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens |
| title_short | Supplementation of Dietary Quercetin and Vitamin E Promotes the Intestinal Structure and Immune Barrier Integrity in Aged Breeder Hens |
| title_sort | supplementation of dietary quercetin and vitamin e promotes the intestinal structure and immune barrier integrity in aged breeder hens |
| topic | chicken antioxidants intestinal immunity inflammation oxidative stress |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.860889/full |
| work_keys_str_mv | AT felixkwameamevor supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT zhifucui supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT xiaxiadu supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT zifanning supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT xundeng supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT danxu supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT gangshu supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT youhaowu supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT xueqingcao supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT weishuo supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT yaofutian supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT diyanli supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT yanwang supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT yaozhang supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT xiaohuidu supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT qingzhu supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT xuehan supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens AT xiaolingzhao supplementationofdietaryquercetinandvitaminepromotestheintestinalstructureandimmunebarrierintegrityinagedbreederhens |