Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400
Abstract.: We examined the involvement of the Na+/Ca2+ exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor, SEA0400. Action potentials were recorded from the myocardial layer of isolated guinea-pig pulmonary vein preparations, and Ca2+ transients were recorded fr...
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| Format: | Article |
| Language: | English |
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Elsevier
2009-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319312204 |
| _version_ | 1818975944221655040 |
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| author | Iyuki Namekata Yayoi Tsuneoka Akira Takahara Hideaki Shimada Takahiko Sugimoto Kiyoshi Takeda Midori Nagaharu Koki Shigenobu Toru Kawanishi Hikaru Tanaka |
| author_facet | Iyuki Namekata Yayoi Tsuneoka Akira Takahara Hideaki Shimada Takahiko Sugimoto Kiyoshi Takeda Midori Nagaharu Koki Shigenobu Toru Kawanishi Hikaru Tanaka |
| author_sort | Iyuki Namekata |
| collection | DOAJ |
| description | Abstract.: We examined the involvement of the Na+/Ca2+ exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor, SEA0400. Action potentials were recorded from the myocardial layer of isolated guinea-pig pulmonary vein preparations, and Ca2+ transients were recorded from the cardiomyocytes. Spontaneous electrical activity was observed in 17.7% of the preparations, which was inhibited by either SEA0400 or ryanodine. In quiescent preparations, ouabain induced electrical activity and spontaneous Ca2+ transients, which were inhibited by SEA0400, as well as ryanodine. These results provide pharmacological evidence that the Na+/Ca2+ exchanger underlies the automaticity of the pulmonary vein myocardium. Keywords:: pulmonary vein myocardium, Na+/Ca2+ exchanger, automaticity |
| first_indexed | 2024-12-20T16:04:00Z |
| format | Article |
| id | doaj.art-f2f92f1eca5449be8d7e14692bb9a152 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-20T16:04:00Z |
| publishDate | 2009-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-f2f92f1eca5449be8d7e14692bb9a1522022-12-21T19:34:11ZengElsevierJournal of Pharmacological Sciences1347-86132009-01-011101111116Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400Iyuki Namekata0Yayoi Tsuneoka1Akira Takahara2Hideaki Shimada3Takahiko Sugimoto4Kiyoshi Takeda5Midori Nagaharu6Koki Shigenobu7Toru Kawanishi8Hikaru Tanaka9Department of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, Japan; Corresponding autnor. iyuki@phar.toho-u.ac.jpDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanDivision of Drugs, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-8501, JapanDepartment of Pharmacology, Toho University Faculty of Pharmaceutical Sciences, Funabashi, Chiba 274-8510, JapanAbstract.: We examined the involvement of the Na+/Ca2+ exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor, SEA0400. Action potentials were recorded from the myocardial layer of isolated guinea-pig pulmonary vein preparations, and Ca2+ transients were recorded from the cardiomyocytes. Spontaneous electrical activity was observed in 17.7% of the preparations, which was inhibited by either SEA0400 or ryanodine. In quiescent preparations, ouabain induced electrical activity and spontaneous Ca2+ transients, which were inhibited by SEA0400, as well as ryanodine. These results provide pharmacological evidence that the Na+/Ca2+ exchanger underlies the automaticity of the pulmonary vein myocardium. Keywords:: pulmonary vein myocardium, Na+/Ca2+ exchanger, automaticityhttp://www.sciencedirect.com/science/article/pii/S1347861319312204 |
| spellingShingle | Iyuki Namekata Yayoi Tsuneoka Akira Takahara Hideaki Shimada Takahiko Sugimoto Kiyoshi Takeda Midori Nagaharu Koki Shigenobu Toru Kawanishi Hikaru Tanaka Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400 Journal of Pharmacological Sciences |
| title | Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400 |
| title_full | Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400 |
| title_fullStr | Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400 |
| title_full_unstemmed | Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400 |
| title_short | Involvement of the Na+/Ca2+ Exchanger in the Automaticity of Guinea-Pig Pulmonary Vein Myocardium as Revealed by SEA0400 |
| title_sort | involvement of the na ca2 exchanger in the automaticity of guinea pig pulmonary vein myocardium as revealed by sea0400 |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319312204 |
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