The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
Background/Aims: The potential role of the two-pore domain potassium channel KCNK5 (also known as TASK-2 and K2P5.1) in activated T cell physiology has only recently been described. So far KCNK5 has been described to be up-regulated in T cells in multiple sclerosis patients and to be implicated in t...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Cell Physiol Biochem Press GmbH & Co KG
2016-02-01
|
Series: | Cellular Physiology and Biochemistry |
Subjects: | |
Online Access: | http://www.karger.com/Article/FullText/443042 |
_version_ | 1819330683720433664 |
---|---|
author | Signe S. Kirkegaard Pernille Dyhl Strøm Steen Gammeltoft Anker Jon Hansen Else K. Hoffmann |
author_facet | Signe S. Kirkegaard Pernille Dyhl Strøm Steen Gammeltoft Anker Jon Hansen Else K. Hoffmann |
author_sort | Signe S. Kirkegaard |
collection | DOAJ |
description | Background/Aims: The potential role of the two-pore domain potassium channel KCNK5 (also known as TASK-2 and K2P5.1) in activated T cell physiology has only recently been described. So far KCNK5 has been described to be up-regulated in T cells in multiple sclerosis patients and to be implicated in the volume regulatory mechanism regulatory volume decrease (RVD) in T cells. Methods: We investigated the time-dependent expression pattern of KCNK5 in CD3/CD28 activated human T cells using qPCR and Western blotting and its role in RVD using a Coulter Counter. Results: KCNK5 is highly up-regulated in CD3/CD28 activated T cells both at mRNA (after 24 h) and protein level (72 and 144 h), but despite this up-regulation the RVD response is inhibited. Furthermore, the swelling-activated Cl- permeability in activated T cells is strongly decreased, and the RVD inhibition is predominantly due to the decreased Cl- permeability. Conclusion: The up-regulated KCNK5 in activated human T cells does not play a volume regulatory role, due to decreased Cl- permeability. We speculate that the KCNK5 up-regulation might play a role in hyperpolarization of the cell membrane leading to increased Ca2+ influx and proliferation of T cells. |
first_indexed | 2024-12-24T14:02:26Z |
format | Article |
id | doaj.art-f2f9fc2e983b426db0ff25d575007485 |
institution | Directory Open Access Journal |
issn | 1015-8987 1421-9778 |
language | English |
last_indexed | 2024-12-24T14:02:26Z |
publishDate | 2016-02-01 |
publisher | Cell Physiol Biochem Press GmbH & Co KG |
record_format | Article |
series | Cellular Physiology and Biochemistry |
spelling | doaj.art-f2f9fc2e983b426db0ff25d5750074852022-12-21T16:52:26ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-02-0138388389210.1159/000443042443042The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is ImpairedSigne S. KirkegaardPernille Dyhl StrømSteen GammeltoftAnker Jon HansenElse K. HoffmannBackground/Aims: The potential role of the two-pore domain potassium channel KCNK5 (also known as TASK-2 and K2P5.1) in activated T cell physiology has only recently been described. So far KCNK5 has been described to be up-regulated in T cells in multiple sclerosis patients and to be implicated in the volume regulatory mechanism regulatory volume decrease (RVD) in T cells. Methods: We investigated the time-dependent expression pattern of KCNK5 in CD3/CD28 activated human T cells using qPCR and Western blotting and its role in RVD using a Coulter Counter. Results: KCNK5 is highly up-regulated in CD3/CD28 activated T cells both at mRNA (after 24 h) and protein level (72 and 144 h), but despite this up-regulation the RVD response is inhibited. Furthermore, the swelling-activated Cl- permeability in activated T cells is strongly decreased, and the RVD inhibition is predominantly due to the decreased Cl- permeability. Conclusion: The up-regulated KCNK5 in activated human T cells does not play a volume regulatory role, due to decreased Cl- permeability. We speculate that the KCNK5 up-regulation might play a role in hyperpolarization of the cell membrane leading to increased Ca2+ influx and proliferation of T cells.http://www.karger.com/Article/FullText/443042KCNK5T cell activationCD3/CD28T cellsRVD |
spellingShingle | Signe S. Kirkegaard Pernille Dyhl Strøm Steen Gammeltoft Anker Jon Hansen Else K. Hoffmann The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired Cellular Physiology and Biochemistry KCNK5 T cell activation CD3/CD28 T cells RVD |
title | The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired |
title_full | The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired |
title_fullStr | The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired |
title_full_unstemmed | The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired |
title_short | The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired |
title_sort | volume activated potassium channel kcnk5 is up regulated in activated human t cells but volume regulation is impaired |
topic | KCNK5 T cell activation CD3/CD28 T cells RVD |
url | http://www.karger.com/Article/FullText/443042 |
work_keys_str_mv | AT signeskirkegaard thevolumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT pernilledyhlstrøm thevolumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT steengammeltoft thevolumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT ankerjonhansen thevolumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT elsekhoffmann thevolumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT signeskirkegaard volumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT pernilledyhlstrøm volumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT steengammeltoft volumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT ankerjonhansen volumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired AT elsekhoffmann volumeactivatedpotassiumchannelkcnk5isupregulatedinactivatedhumantcellsbutvolumeregulationisimpaired |