The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired

Background/Aims: The potential role of the two-pore domain potassium channel KCNK5 (also known as TASK-2 and K2P5.1) in activated T cell physiology has only recently been described. So far KCNK5 has been described to be up-regulated in T cells in multiple sclerosis patients and to be implicated in t...

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Main Authors: Signe S. Kirkegaard, Pernille Dyhl Strøm, Steen Gammeltoft, Anker Jon Hansen, Else K. Hoffmann
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2016-02-01
Series:Cellular Physiology and Biochemistry
Subjects:
Online Access:http://www.karger.com/Article/FullText/443042
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author Signe S. Kirkegaard
Pernille Dyhl Strøm
Steen Gammeltoft
Anker Jon Hansen
Else K. Hoffmann
author_facet Signe S. Kirkegaard
Pernille Dyhl Strøm
Steen Gammeltoft
Anker Jon Hansen
Else K. Hoffmann
author_sort Signe S. Kirkegaard
collection DOAJ
description Background/Aims: The potential role of the two-pore domain potassium channel KCNK5 (also known as TASK-2 and K2P5.1) in activated T cell physiology has only recently been described. So far KCNK5 has been described to be up-regulated in T cells in multiple sclerosis patients and to be implicated in the volume regulatory mechanism regulatory volume decrease (RVD) in T cells. Methods: We investigated the time-dependent expression pattern of KCNK5 in CD3/CD28 activated human T cells using qPCR and Western blotting and its role in RVD using a Coulter Counter. Results: KCNK5 is highly up-regulated in CD3/CD28 activated T cells both at mRNA (after 24 h) and protein level (72 and 144 h), but despite this up-regulation the RVD response is inhibited. Furthermore, the swelling-activated Cl- permeability in activated T cells is strongly decreased, and the RVD inhibition is predominantly due to the decreased Cl- permeability. Conclusion: The up-regulated KCNK5 in activated human T cells does not play a volume regulatory role, due to decreased Cl- permeability. We speculate that the KCNK5 up-regulation might play a role in hyperpolarization of the cell membrane leading to increased Ca2+ influx and proliferation of T cells.
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spelling doaj.art-f2f9fc2e983b426db0ff25d5750074852022-12-21T16:52:26ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782016-02-0138388389210.1159/000443042443042The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is ImpairedSigne S. KirkegaardPernille Dyhl StrømSteen GammeltoftAnker Jon HansenElse K. HoffmannBackground/Aims: The potential role of the two-pore domain potassium channel KCNK5 (also known as TASK-2 and K2P5.1) in activated T cell physiology has only recently been described. So far KCNK5 has been described to be up-regulated in T cells in multiple sclerosis patients and to be implicated in the volume regulatory mechanism regulatory volume decrease (RVD) in T cells. Methods: We investigated the time-dependent expression pattern of KCNK5 in CD3/CD28 activated human T cells using qPCR and Western blotting and its role in RVD using a Coulter Counter. Results: KCNK5 is highly up-regulated in CD3/CD28 activated T cells both at mRNA (after 24 h) and protein level (72 and 144 h), but despite this up-regulation the RVD response is inhibited. Furthermore, the swelling-activated Cl- permeability in activated T cells is strongly decreased, and the RVD inhibition is predominantly due to the decreased Cl- permeability. Conclusion: The up-regulated KCNK5 in activated human T cells does not play a volume regulatory role, due to decreased Cl- permeability. We speculate that the KCNK5 up-regulation might play a role in hyperpolarization of the cell membrane leading to increased Ca2+ influx and proliferation of T cells.http://www.karger.com/Article/FullText/443042KCNK5T cell activationCD3/CD28T cellsRVD
spellingShingle Signe S. Kirkegaard
Pernille Dyhl Strøm
Steen Gammeltoft
Anker Jon Hansen
Else K. Hoffmann
The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
Cellular Physiology and Biochemistry
KCNK5
T cell activation
CD3/CD28
T cells
RVD
title The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
title_full The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
title_fullStr The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
title_full_unstemmed The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
title_short The Volume Activated Potassium Channel KCNK5 is Up-Regulated in Activated Human T Cells, but Volume Regulation is Impaired
title_sort volume activated potassium channel kcnk5 is up regulated in activated human t cells but volume regulation is impaired
topic KCNK5
T cell activation
CD3/CD28
T cells
RVD
url http://www.karger.com/Article/FullText/443042
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