Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis
Summary: An emerging topic in virology is that viral replication is closely linked with the metabolic reprogramming of host cells. Understanding the effects of reprogramming host cell metabolism due to classical swine fever virus (CSFV) infection and the underling mechanisms would facilitate control...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-11-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422201625X |
| _version_ | 1811247919658958848 |
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| author | Xiaodong Zou Yang Yang Feng Lin Jiahuan Chen Huanyu Zhang Linquan Li Hongsheng Ouyang Daxin Pang Linzhu Ren Xiaochun Tang |
| author_facet | Xiaodong Zou Yang Yang Feng Lin Jiahuan Chen Huanyu Zhang Linquan Li Hongsheng Ouyang Daxin Pang Linzhu Ren Xiaochun Tang |
| author_sort | Xiaodong Zou |
| collection | DOAJ |
| description | Summary: An emerging topic in virology is that viral replication is closely linked with the metabolic reprogramming of host cells. Understanding the effects of reprogramming host cell metabolism due to classical swine fever virus (CSFV) infection and the underling mechanisms would facilitate controlling the spread of classical swine fever (CSF). In the current study, we found that CSFV infection enhanced aerobic glycolysis in PK-15 cells. Blocking glycolysis with 2-deoxy-d-glycose or disrupting the enzymes PFKL and LDHA decreased CSFV replication. Lactate was identified as an important molecule in CSFV replication, independent of the pentose phosphate pathway and tricarboxylic acid cycle. Further analysis demonstrated that the accumulated lactate in cells promoted cholesterol biosynthesis, which facilitated CSFV replication and disrupted the type I interferon response during CSFV replication, and the disruption of cholesterol synthesis abolished the lactate effects on CSFV replication. The results provided more insights into the complex pathological mechanisms of CSFV. |
| first_indexed | 2024-04-12T15:17:55Z |
| format | Article |
| id | doaj.art-f2fa7d40c14f445b9a945721e59532ad |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-04-12T15:17:55Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-f2fa7d40c14f445b9a945721e59532ad2022-12-22T03:27:33ZengElsevieriScience2589-00422022-11-012511105353Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesisXiaodong Zou0Yang Yang1Feng Lin2Jiahuan Chen3Huanyu Zhang4Linquan Li5Hongsheng Ouyang6Daxin Pang7Linzhu Ren8Xiaochun Tang9College of Animal Sciences, Jilin University, Changchun, ChinaCollege of Animal Sciences, Jilin University, Changchun, ChinaCollege of Animal Sciences, Jilin University, Changchun, ChinaCollege of Animal Sciences, Jilin University, Changchun, ChinaCollege of Animal Sciences, Jilin University, Changchun, ChinaCollege of Animal Sciences, Jilin University, Changchun, ChinaCollege of Animal Sciences, Jilin University, Changchun, China; Chongqing Research Institute of Jilin University, Chongqing, ChinaCollege of Animal Sciences, Jilin University, Changchun, China; Chongqing Research Institute of Jilin University, Chongqing, ChinaCollege of Animal Sciences, Jilin University, Changchun, China; Corresponding authorCollege of Animal Sciences, Jilin University, Changchun, China; Chongqing Research Institute of Jilin University, Chongqing, China; Corresponding authorSummary: An emerging topic in virology is that viral replication is closely linked with the metabolic reprogramming of host cells. Understanding the effects of reprogramming host cell metabolism due to classical swine fever virus (CSFV) infection and the underling mechanisms would facilitate controlling the spread of classical swine fever (CSF). In the current study, we found that CSFV infection enhanced aerobic glycolysis in PK-15 cells. Blocking glycolysis with 2-deoxy-d-glycose or disrupting the enzymes PFKL and LDHA decreased CSFV replication. Lactate was identified as an important molecule in CSFV replication, independent of the pentose phosphate pathway and tricarboxylic acid cycle. Further analysis demonstrated that the accumulated lactate in cells promoted cholesterol biosynthesis, which facilitated CSFV replication and disrupted the type I interferon response during CSFV replication, and the disruption of cholesterol synthesis abolished the lactate effects on CSFV replication. The results provided more insights into the complex pathological mechanisms of CSFV.http://www.sciencedirect.com/science/article/pii/S258900422201625XVirologyHuman metabolism |
| spellingShingle | Xiaodong Zou Yang Yang Feng Lin Jiahuan Chen Huanyu Zhang Linquan Li Hongsheng Ouyang Daxin Pang Linzhu Ren Xiaochun Tang Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis iScience Virology Human metabolism |
| title | Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis |
| title_full | Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis |
| title_fullStr | Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis |
| title_full_unstemmed | Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis |
| title_short | Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis |
| title_sort | lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis |
| topic | Virology Human metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S258900422201625X |
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