Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line
BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensi...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e200113.pdf |
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| author | Luzia Monteiro de Castro Côrtes Daniela de Pita-Pereira Priscila Silva Grijó Farani Bernardo Acácio Santini Pereira Geovane Dias-Lopes Franklin Souza da Silva Paloma Resende Corrêa Roger Magno Macedo Silva Suzana Côrte-Real Felio Jesus Bello Leila Mendonça-Lima Otacilio da Cruz Moreira Mariana Caldas Waghabi Carlos Roberto Alves |
| author_facet | Luzia Monteiro de Castro Côrtes Daniela de Pita-Pereira Priscila Silva Grijó Farani Bernardo Acácio Santini Pereira Geovane Dias-Lopes Franklin Souza da Silva Paloma Resende Corrêa Roger Magno Macedo Silva Suzana Côrte-Real Felio Jesus Bello Leila Mendonça-Lima Otacilio da Cruz Moreira Mariana Caldas Waghabi Carlos Roberto Alves |
| author_sort | Luzia Monteiro de Castro Côrtes |
| collection | DOAJ |
| description | BACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. METHODS Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins using Mascot search and, afterwards, resulting sequences were applied for analysis with VectorBase. RESULTS Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23%), proteases (14%), cytoskeletal proteins (11%) and interactive membrane proteins (9.5%). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin and vacuolar transport protein) were observed after an eight-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes: LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). CONCLUSIONS This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression. |
| first_indexed | 2024-03-12T18:07:53Z |
| format | Article |
| id | doaj.art-f2fba11b1eea41859f2afd76ab8d6bfd |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:07:53Z |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-f2fba11b1eea41859f2afd76ab8d6bfd2023-08-02T09:25:57ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-806010.1590/0074-02760200113Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell lineLuzia Monteiro de Castro CôrtesDaniela de Pita-PereiraPriscila Silva Grijó FaraniBernardo Acácio Santini PereiraGeovane Dias-LopesFranklin Souza da SilvaPaloma Resende CorrêaRoger Magno Macedo SilvaSuzana Côrte-RealFelio Jesus BelloLeila Mendonça-LimaOtacilio da Cruz MoreiraMariana Caldas WaghabiCarlos Roberto AlvesBACKGROUND Lutzomyia longipalpis-derived cell line (Lulo) has been suggested as a model for studies of interaction between sandflies and Leishmania. OBJECTIVES Here, we present data of proteomic and gene expression analyses of Lulo cell related to interactions with Leishmania (Viannia) braziliensis. METHODS Lulo cell protein extracts were analysed through a combination of two-dimensional gel electrophoresis and mass spectrometry and resulting spots were further investigated in silico to identify proteins using Mascot search and, afterwards, resulting sequences were applied for analysis with VectorBase. RESULTS Sixty-four spots were identified showing similarities to other proteins registered in the databases and could be classified according to their biological function, such as ion-binding proteins (23%), proteases (14%), cytoskeletal proteins (11%) and interactive membrane proteins (9.5%). Effects of interaction with L. (V.) braziliensis with the expression of three genes (enolase, tubulin and vacuolar transport protein) were observed after an eight-hour timeframe and compared to culture without parasites, and demonstrated the impact of parasite interaction with the expression of the following genes: LLOJ000219 (1.69-fold), LLOJ000326 (1.43-fold) and LLOJ006663 (2.41-fold). CONCLUSIONS This set of results adds relevant information regarding the usefulness of the Lulo cell line for studies with Leishmania parasites that indicate variations of protein expression.http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e200113.pdfLulo cellLeishmania (Viannia) braziliensisproteomicRT-qPCR |
| spellingShingle | Luzia Monteiro de Castro Côrtes Daniela de Pita-Pereira Priscila Silva Grijó Farani Bernardo Acácio Santini Pereira Geovane Dias-Lopes Franklin Souza da Silva Paloma Resende Corrêa Roger Magno Macedo Silva Suzana Côrte-Real Felio Jesus Bello Leila Mendonça-Lima Otacilio da Cruz Moreira Mariana Caldas Waghabi Carlos Roberto Alves Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line Memorias do Instituto Oswaldo Cruz Lulo cell Leishmania (Viannia) braziliensis proteomic RT-qPCR |
| title | Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line |
| title_full | Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line |
| title_fullStr | Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line |
| title_full_unstemmed | Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line |
| title_short | Insights into the proteomic profile and gene expression of Lutzomyia longipalpis-derived Lulo cell line |
| title_sort | insights into the proteomic profile and gene expression of lutzomyia longipalpis derived lulo cell line |
| topic | Lulo cell Leishmania (Viannia) braziliensis proteomic RT-qPCR |
| url | http://www.scielo.br/pdf/mioc/v115/1678-8060-mioc-115-e200113.pdf |
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