A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum
Fusarium graminearum (teleomorph: Gibberella zeae) is an Ascomycete fungal plant pathogen which infects a range of agriculturally important crops, including wheat, barley, and maize. A random plasmid insertion mutagenesis approach was used to analyze the pathogenicity of the PH-1 strain, for which f...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2010-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-8-1083 |
| _version_ | 1811287474017665024 |
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| author | Thomas K. Baldwin Iffa Gaffoor John Antoniw Corrie Andries John Guenther Martin Urban Heather E. Hallen-Adams John Pitkin Kim E. Hammond-Kosack Frances Trail |
| author_facet | Thomas K. Baldwin Iffa Gaffoor John Antoniw Corrie Andries John Guenther Martin Urban Heather E. Hallen-Adams John Pitkin Kim E. Hammond-Kosack Frances Trail |
| author_sort | Thomas K. Baldwin |
| collection | DOAJ |
| description | Fusarium graminearum (teleomorph: Gibberella zeae) is an Ascomycete fungal plant pathogen which infects a range of agriculturally important crops, including wheat, barley, and maize. A random plasmid insertion mutagenesis approach was used to analyze the pathogenicity of the PH-1 strain, for which full genomic information is available. Fungal transformants were initially screened for their ability to infect wheat ears. From a total of 1,170 transformants screened, eight were confirmed to be highly reduced in pathogenicity toward wheat ears and roots. These were designated disease-attenuated F. graminearum (daf) mutants. The in vitro growth rate and appearance of each daf mutant was equivalent to the parental strain. Deoxynivalenol (DON) was not detected in threshed grain recovered from ears inoculated with the daf10 mutant. Plasmid rescue and sequencing of the mutant daf10 revealed a deletion of approximately 350 kb from one end of chromosome 1. This chromosome segment is predicted to contain 146 genes. Microarray analysis of daf10 gene expression during growth in DON-inducing conditions confirmed the large deletion. The identities of the genes deleted and their potential role in DON production, pathogenesis, and other life processes are discussed. |
| first_indexed | 2024-04-13T03:19:07Z |
| format | Article |
| id | doaj.art-f2fce7c418b646c9b64291944bae7bbf |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:19:07Z |
| publishDate | 2010-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-f2fce7c418b646c9b64291944bae7bbf2022-12-22T03:04:50ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062010-08-012381083109610.1094/MPMI-23-8-1083A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearumThomas K. BaldwinIffa GaffoorJohn AntoniwCorrie AndriesJohn GuentherMartin UrbanHeather E. Hallen-AdamsJohn PitkinKim E. Hammond-KosackFrances TrailFusarium graminearum (teleomorph: Gibberella zeae) is an Ascomycete fungal plant pathogen which infects a range of agriculturally important crops, including wheat, barley, and maize. A random plasmid insertion mutagenesis approach was used to analyze the pathogenicity of the PH-1 strain, for which full genomic information is available. Fungal transformants were initially screened for their ability to infect wheat ears. From a total of 1,170 transformants screened, eight were confirmed to be highly reduced in pathogenicity toward wheat ears and roots. These were designated disease-attenuated F. graminearum (daf) mutants. The in vitro growth rate and appearance of each daf mutant was equivalent to the parental strain. Deoxynivalenol (DON) was not detected in threshed grain recovered from ears inoculated with the daf10 mutant. Plasmid rescue and sequencing of the mutant daf10 revealed a deletion of approximately 350 kb from one end of chromosome 1. This chromosome segment is predicted to contain 146 genes. Microarray analysis of daf10 gene expression during growth in DON-inducing conditions confirmed the large deletion. The identities of the genes deleted and their potential role in DON production, pathogenesis, and other life processes are discussed.https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-8-1083 |
| spellingShingle | Thomas K. Baldwin Iffa Gaffoor John Antoniw Corrie Andries John Guenther Martin Urban Heather E. Hallen-Adams John Pitkin Kim E. Hammond-Kosack Frances Trail A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum Molecular Plant-Microbe Interactions |
| title | A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum |
| title_full | A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum |
| title_fullStr | A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum |
| title_full_unstemmed | A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum |
| title_short | A Partial Chromosomal Deletion Caused by Random Plasmid Integration Resulted in a Reduced Virulence Phenotype in Fusarium graminearum |
| title_sort | partial chromosomal deletion caused by random plasmid integration resulted in a reduced virulence phenotype in fusarium graminearum |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-8-1083 |
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