Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.
Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause sev...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0249617 |
| _version_ | 1819039103343132672 |
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| author | Mariela E Srednik Kristina Lantz Jessica A Hicks Brenda R Morningstar-Shaw Tonya A Mackie Linda K Schlater |
| author_facet | Mariela E Srednik Kristina Lantz Jessica A Hicks Brenda R Morningstar-Shaw Tonya A Mackie Linda K Schlater |
| author_sort | Mariela E Srednik |
| collection | DOAJ |
| description | Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014-2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3'')-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans. |
| first_indexed | 2024-12-21T08:47:53Z |
| format | Article |
| id | doaj.art-f2fdeda4b687467f8a963b94c19861d2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T08:47:53Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f2fdeda4b687467f8a963b94c19861d22022-12-21T19:09:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-01169e024961710.1371/journal.pone.0249617Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States.Mariela E SrednikKristina LantzJessica A HicksBrenda R Morningstar-ShawTonya A MackieLinda K SchlaterSalmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014-2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3'')-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans.https://doi.org/10.1371/journal.pone.0249617 |
| spellingShingle | Mariela E Srednik Kristina Lantz Jessica A Hicks Brenda R Morningstar-Shaw Tonya A Mackie Linda K Schlater Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States. PLoS ONE |
| title | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States. |
| title_full | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States. |
| title_fullStr | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States. |
| title_full_unstemmed | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States. |
| title_short | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States. |
| title_sort | antimicrobial resistance and genomic characterization of salmonella dublin isolates in cattle from the united states |
| url | https://doi.org/10.1371/journal.pone.0249617 |
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