Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo
Mycobacterium tuberculosis clinical strains usually possess traits different from the laboratory strains like H37Rv, especially those clinical drug resistant strains. With whole genome and transcriptome sequencing, we depicted the feature of two multi-drug resistant Mtb strains in resistance and vir...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.00219/full |
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| author | Jun Tang Jun Tang Jun Tang Jun Tang Jun Tang Zhihao Liu Zhihao Liu Zhihao Liu Zhihao Liu Zhihao Liu Ya'nan Shi Ya'nan Shi Ya'nan Shi Ya'nan Shi Ya'nan Shi Lingjun Zhan Lingjun Zhan Lingjun Zhan Lingjun Zhan Lingjun Zhan Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin |
| author_facet | Jun Tang Jun Tang Jun Tang Jun Tang Jun Tang Zhihao Liu Zhihao Liu Zhihao Liu Zhihao Liu Zhihao Liu Ya'nan Shi Ya'nan Shi Ya'nan Shi Ya'nan Shi Ya'nan Shi Lingjun Zhan Lingjun Zhan Lingjun Zhan Lingjun Zhan Lingjun Zhan Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin |
| author_sort | Jun Tang |
| collection | DOAJ |
| description | Mycobacterium tuberculosis clinical strains usually possess traits different from the laboratory strains like H37Rv, especially those clinical drug resistant strains. With whole genome and transcriptome sequencing, we depicted the feature of two multi-drug resistant Mtb strains in resistance and virulence. Compared with H37Rv, the differential expressed genes (DEGs) of the MDR strains showed featured enrichment in arginine biosynthesis, fatty acid biosynthesis, and metabolism pathway. In the subset of virulence genes, the overlapping DEGs of the MDR strains exhibited downregulation of the cluster in type VII secretion system. In the mice experiment, the MDR strains showed attenuated but distinct virulence, both in survival rate and pathology. Taken together, the whole genome and transcriptome analysis could help understand the unique feature of the MDR strains both in resistance and virulence. |
| first_indexed | 2024-12-10T07:56:47Z |
| format | Article |
| id | doaj.art-f3002887a1eb409d86597352a1df0632 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T07:56:47Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-f3002887a1eb409d86597352a1df06322022-12-22T01:56:54ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-05-011010.3389/fcimb.2020.00219514005Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivoJun Tang0Jun Tang1Jun Tang2Jun Tang3Jun Tang4Zhihao Liu5Zhihao Liu6Zhihao Liu7Zhihao Liu8Zhihao Liu9Ya'nan Shi10Ya'nan Shi11Ya'nan Shi12Ya'nan Shi13Ya'nan Shi14Lingjun Zhan15Lingjun Zhan16Lingjun Zhan17Lingjun Zhan18Lingjun Zhan19Chuan Qin20Chuan Qin21Chuan Qin22Chuan Qin23Chuan Qin24Institute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Beijing, ChinaBeijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, ChinaTuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Beijing, ChinaBeijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, ChinaTuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Beijing, ChinaBeijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, ChinaTuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Beijing, ChinaBeijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, ChinaTuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences (CAMS), Comparative Medicine Center, Peking Union Medical College (PUMC), Beijing, ChinaNHC Key Laboratory of Human Disease Comparative Medicine, Beijing, ChinaBeijing Key Laboratory for Animal Models of Emerging and Reemerging Infectious, Beijing, ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Beijing, ChinaTuberculosis Center, Chinese Academy of Medical Sciences (CAMS), Beijing, ChinaMycobacterium tuberculosis clinical strains usually possess traits different from the laboratory strains like H37Rv, especially those clinical drug resistant strains. With whole genome and transcriptome sequencing, we depicted the feature of two multi-drug resistant Mtb strains in resistance and virulence. Compared with H37Rv, the differential expressed genes (DEGs) of the MDR strains showed featured enrichment in arginine biosynthesis, fatty acid biosynthesis, and metabolism pathway. In the subset of virulence genes, the overlapping DEGs of the MDR strains exhibited downregulation of the cluster in type VII secretion system. In the mice experiment, the MDR strains showed attenuated but distinct virulence, both in survival rate and pathology. Taken together, the whole genome and transcriptome analysis could help understand the unique feature of the MDR strains both in resistance and virulence.https://www.frontiersin.org/article/10.3389/fcimb.2020.00219/fullwhole genome re-sequencingRNA-seqtranscriptome sequencingMycobacterium tuberculosismulti-drug resistantvirulence |
| spellingShingle | Jun Tang Jun Tang Jun Tang Jun Tang Jun Tang Zhihao Liu Zhihao Liu Zhihao Liu Zhihao Liu Zhihao Liu Ya'nan Shi Ya'nan Shi Ya'nan Shi Ya'nan Shi Ya'nan Shi Lingjun Zhan Lingjun Zhan Lingjun Zhan Lingjun Zhan Lingjun Zhan Chuan Qin Chuan Qin Chuan Qin Chuan Qin Chuan Qin Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo Frontiers in Cellular and Infection Microbiology whole genome re-sequencing RNA-seq transcriptome sequencing Mycobacterium tuberculosis multi-drug resistant virulence |
| title | Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo |
| title_full | Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo |
| title_fullStr | Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo |
| title_full_unstemmed | Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo |
| title_short | Whole Genome and Transcriptome Sequencing of Two Multi-Drug Resistant Mycobacterium tuberculosis Strains to Facilitate Illustrating Their Virulence in vivo |
| title_sort | whole genome and transcriptome sequencing of two multi drug resistant mycobacterium tuberculosis strains to facilitate illustrating their virulence in vivo |
| topic | whole genome re-sequencing RNA-seq transcriptome sequencing Mycobacterium tuberculosis multi-drug resistant virulence |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2020.00219/full |
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