The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats
The purpose of this study was to observe the effects of a novel combination of inositol-stabilized arginine silicate complex (ASI) and magnesium biotinate (MgB) on the prevention of skin damage after UVB exposure in rats. Forty-nine Sprague-Dawley rats were randomized into one of the following group...
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Frontiers Media S.A.
2021-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.657207/full |
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| author | Demet Cicek Betul Demir Cemal Orhan Mehmet Tuzcu Ibrahim Hanifi Ozercan Nurhan Sahin James Komorowski Sara Perez Ojalvo Sarah Sylla Kazim Sahin |
| author_facet | Demet Cicek Betul Demir Cemal Orhan Mehmet Tuzcu Ibrahim Hanifi Ozercan Nurhan Sahin James Komorowski Sara Perez Ojalvo Sarah Sylla Kazim Sahin |
| author_sort | Demet Cicek |
| collection | DOAJ |
| description | The purpose of this study was to observe the effects of a novel combination of inositol-stabilized arginine silicate complex (ASI) and magnesium biotinate (MgB) on the prevention of skin damage after UVB exposure in rats. Forty-nine Sprague-Dawley rats were randomized into one of the following groups: (1) NC, normal control, (2) SC, shaved control, (3) UVB (exposed to UVB radiation), (4) ASI+MgB-L (Low Dose), (5) ASI+MgB-H (High Dose), (6) ASI+MgB-L+MgB cream, (7) ASI+MgB-H+MgB cream. The results showed that ASI+MgB treatment alleviated the macroscopic and histopathological damages in the skin of rats caused by UVB exposure. Skin elasticity evaluation showed a similar trend. ASI+MgB increased serum Mg, Fe, Zn, Cu, Si, biotin, and arginine concentrations and skin hydroxyproline and biotinidase levels while decreasing skin elastase activity (p < 0.05) and malondialdehyde (MDA) concentration (p < 0.001). Moreover, ASI+MgB treatment increased skin levels of biotin-dependent carboxylases (ACC1, ACC2, PC, PCC, MCC) and decreased mammalian target of rapamycin (mTOR) pathways and matrix metalloproteinase protein levels by the regulation of the activator protein 1 (AP-1), and mitogen activated protein kinases (MAPKs) signaling pathways. In addition, ASI+MgB caused lower levels of inflammatory factors, including TNF-α, NFκB, IL-6, IL-8, and COX-2 in the skin samples (p < 0.05). The levels of Bax and caspase-3 were increased, while anti-apoptotic protein Bcl-2 was decreased by UVB exposure, which was reversed by ASI+MgB treatment. These results show that treatment with ASI and MgB protects against skin damage by improving skin appearance, elasticity, inflammation, apoptosis, and overall health. |
| first_indexed | 2024-12-21T20:03:01Z |
| format | Article |
| id | doaj.art-f30074e3dad6495fb901218d529c33bf |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-21T20:03:01Z |
| publishDate | 2021-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj.art-f30074e3dad6495fb901218d529c33bf2022-12-21T18:51:56ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-06-011210.3389/fphar.2021.657207657207The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in RatsDemet Cicek0Betul Demir1Cemal Orhan2Mehmet Tuzcu3Ibrahim Hanifi Ozercan4Nurhan Sahin5James Komorowski6Sara Perez Ojalvo7Sarah Sylla8Kazim Sahin9Department of Dermatology, Faculty of Medicine, Firat University, Elazig, TurkeyDepartment of Dermatology, Faculty of Medicine, Firat University, Elazig, TurkeyDepartment of Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, TurkeyDepartment of Biology, Faculty of Science, Firat University, Elazig, TurkeyDepartment of Pathology, Faculty of Medicine, Firat University, Elazig, TurkeyDepartment of Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, TurkeyResearch and Development, JDS Therapeutics, LLC, Harrison, NY, United StatesResearch and Development, JDS Therapeutics, LLC, Harrison, NY, United StatesResearch and Development, JDS Therapeutics, LLC, Harrison, NY, United StatesDepartment of Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, TurkeyThe purpose of this study was to observe the effects of a novel combination of inositol-stabilized arginine silicate complex (ASI) and magnesium biotinate (MgB) on the prevention of skin damage after UVB exposure in rats. Forty-nine Sprague-Dawley rats were randomized into one of the following groups: (1) NC, normal control, (2) SC, shaved control, (3) UVB (exposed to UVB radiation), (4) ASI+MgB-L (Low Dose), (5) ASI+MgB-H (High Dose), (6) ASI+MgB-L+MgB cream, (7) ASI+MgB-H+MgB cream. The results showed that ASI+MgB treatment alleviated the macroscopic and histopathological damages in the skin of rats caused by UVB exposure. Skin elasticity evaluation showed a similar trend. ASI+MgB increased serum Mg, Fe, Zn, Cu, Si, biotin, and arginine concentrations and skin hydroxyproline and biotinidase levels while decreasing skin elastase activity (p < 0.05) and malondialdehyde (MDA) concentration (p < 0.001). Moreover, ASI+MgB treatment increased skin levels of biotin-dependent carboxylases (ACC1, ACC2, PC, PCC, MCC) and decreased mammalian target of rapamycin (mTOR) pathways and matrix metalloproteinase protein levels by the regulation of the activator protein 1 (AP-1), and mitogen activated protein kinases (MAPKs) signaling pathways. In addition, ASI+MgB caused lower levels of inflammatory factors, including TNF-α, NFκB, IL-6, IL-8, and COX-2 in the skin samples (p < 0.05). The levels of Bax and caspase-3 were increased, while anti-apoptotic protein Bcl-2 was decreased by UVB exposure, which was reversed by ASI+MgB treatment. These results show that treatment with ASI and MgB protects against skin damage by improving skin appearance, elasticity, inflammation, apoptosis, and overall health.https://www.frontiersin.org/articles/10.3389/fphar.2021.657207/fullUVBskinarginine silicatemagnesiumbiotin |
| spellingShingle | Demet Cicek Betul Demir Cemal Orhan Mehmet Tuzcu Ibrahim Hanifi Ozercan Nurhan Sahin James Komorowski Sara Perez Ojalvo Sarah Sylla Kazim Sahin The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats Frontiers in Pharmacology UVB skin arginine silicate magnesium biotin |
| title | The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats |
| title_full | The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats |
| title_fullStr | The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats |
| title_full_unstemmed | The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats |
| title_short | The Protective Effects of a Combination of an Arginine Silicate Complex and Magnesium Biotinate Against UV-Induced Skin Damage in Rats |
| title_sort | protective effects of a combination of an arginine silicate complex and magnesium biotinate against uv induced skin damage in rats |
| topic | UVB skin arginine silicate magnesium biotin |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.657207/full |
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