Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it>
<p>Abstract</p> <p>Background</p> <p>F<sub>1</sub> hybrid clones of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> are widely grown for pulp and paper production in tropical and subtropical regions. Volume growth and wood quali...
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | BMC Genetics |
| Online Access: | http://www.biomedcentral.com/1471-2156/13/60 |
| _version_ | 1818228942310473728 |
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| author | Kullan Anand Raj van Dyk Maria M Hefer Charles A Jones Nicoletta Kanzler Arnulf Myburg Alexander A |
| author_facet | Kullan Anand Raj van Dyk Maria M Hefer Charles A Jones Nicoletta Kanzler Arnulf Myburg Alexander A |
| author_sort | Kullan Anand Raj |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>F<sub>1</sub> hybrid clones of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> are widely grown for pulp and paper production in tropical and subtropical regions. Volume growth and wood quality are priority objectives in <it>Eucalyptus</it> tree improvement. The molecular basis of quantitative variation and trait expression in eucalypt hybrids, however, remains largely unknown. The recent availability of a draft genome sequence (<url>http://www.phytozome.net</url>) and genome-wide genotyping platforms, combined with high levels of genetic variation and high linkage disequilibrium in hybrid crosses, greatly facilitate the detection of quantitative trait loci (QTLs) as well as underlying candidate genes for growth and wood property traits. In this study, we used Diversity Arrays Technology markers to assess the genetic architecture of volume growth (diameter at breast height, DBH) and wood basic density in four-year-old progeny of an interspecific backcross pedigree of <it>E. grandis</it> and <it>E. urophylla</it>. In addition, we used Illumina RNA-Seq expression profiling in the <it>E. urophylla</it> backcross family to identify cis- and trans-acting polymorphisms (eQTLs) affecting transcript abundance of genes underlying QTLs for wood basic density.</p> <p>Results</p> <p>A total of five QTLs for DBH and 12 for wood basic density were identified in the two backcross families. Individual QTLs for DBH and wood basic density explained 3.1 to 12.2% of phenotypic variation. Candidate genes underlying QTLs for wood basic density on linkage groups 8 and 9 were found to share trans-acting eQTLs located on linkage groups 4 and 10, which in turn coincided with QTLs for wood basic density suggesting that these QTLs represent segregating components of an underlying transcriptional network.</p> <p>Conclusion</p> <p>This is the first demonstration of the use of next-generation expression profiling to quantify transcript abundance in a segregating tree population and identify candidate genes potentially affecting wood property variation. The QTLs identified in this study provide a resource for identifying candidate genes and developing molecular markers for marker-assisted breeding of volume growth and wood basic density. Our results suggest that integrated analysis of transcript and trait variation in eucalypt hybrids can be used to dissect the molecular basis of quantitative variation in wood property traits.</p> |
| first_indexed | 2024-12-12T10:10:43Z |
| format | Article |
| id | doaj.art-f301651e37d846fa94b4eacb507ee36e |
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| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-12-12T10:10:43Z |
| publishDate | 2012-07-01 |
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| spelling | doaj.art-f301651e37d846fa94b4eacb507ee36e2022-12-22T00:27:48ZengBMCBMC Genetics1471-21562012-07-011316010.1186/1471-2156-13-60Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it>Kullan Anand Rajvan Dyk Maria MHefer Charles AJones NicolettaKanzler ArnulfMyburg Alexander A<p>Abstract</p> <p>Background</p> <p>F<sub>1</sub> hybrid clones of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> are widely grown for pulp and paper production in tropical and subtropical regions. Volume growth and wood quality are priority objectives in <it>Eucalyptus</it> tree improvement. The molecular basis of quantitative variation and trait expression in eucalypt hybrids, however, remains largely unknown. The recent availability of a draft genome sequence (<url>http://www.phytozome.net</url>) and genome-wide genotyping platforms, combined with high levels of genetic variation and high linkage disequilibrium in hybrid crosses, greatly facilitate the detection of quantitative trait loci (QTLs) as well as underlying candidate genes for growth and wood property traits. In this study, we used Diversity Arrays Technology markers to assess the genetic architecture of volume growth (diameter at breast height, DBH) and wood basic density in four-year-old progeny of an interspecific backcross pedigree of <it>E. grandis</it> and <it>E. urophylla</it>. In addition, we used Illumina RNA-Seq expression profiling in the <it>E. urophylla</it> backcross family to identify cis- and trans-acting polymorphisms (eQTLs) affecting transcript abundance of genes underlying QTLs for wood basic density.</p> <p>Results</p> <p>A total of five QTLs for DBH and 12 for wood basic density were identified in the two backcross families. Individual QTLs for DBH and wood basic density explained 3.1 to 12.2% of phenotypic variation. Candidate genes underlying QTLs for wood basic density on linkage groups 8 and 9 were found to share trans-acting eQTLs located on linkage groups 4 and 10, which in turn coincided with QTLs for wood basic density suggesting that these QTLs represent segregating components of an underlying transcriptional network.</p> <p>Conclusion</p> <p>This is the first demonstration of the use of next-generation expression profiling to quantify transcript abundance in a segregating tree population and identify candidate genes potentially affecting wood property variation. The QTLs identified in this study provide a resource for identifying candidate genes and developing molecular markers for marker-assisted breeding of volume growth and wood basic density. Our results suggest that integrated analysis of transcript and trait variation in eucalypt hybrids can be used to dissect the molecular basis of quantitative variation in wood property traits.</p>http://www.biomedcentral.com/1471-2156/13/60 |
| spellingShingle | Kullan Anand Raj van Dyk Maria M Hefer Charles A Jones Nicoletta Kanzler Arnulf Myburg Alexander A Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> BMC Genetics |
| title | Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> |
| title_full | Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> |
| title_fullStr | Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> |
| title_full_unstemmed | Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> |
| title_short | Genetic dissection of growth, wood basic density and gene expression in interspecific backcrosses of <it>Eucalyptus grandis</it> and <it>E. urophylla</it> |
| title_sort | genetic dissection of growth wood basic density and gene expression in interspecific backcrosses of it eucalyptus grandis it and it e urophylla it |
| url | http://www.biomedcentral.com/1471-2156/13/60 |
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