miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone
Summary: Coordination between osteogenesis and angiogenesis is required for bone homeostasis. Here, we show that miR-29cb2 is a bone-specific miRNA and plays critical roles on angiogenesis-osteogenesis coupling during bone remodeling. Mice with deletion of miR-29cb2 exhibit osteopenic phenotypes and...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221015741 |
| _version_ | 1819261415598325760 |
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| author | Liping Ouyang Yingxiao Sun Dan Lv Xiaochun Peng Xiaoming Liu Lei Ci Guoning Zhang Bo Yuan Ling Li Jian Fei Jun Ma Xuanyong Liu Yun Liao |
| author_facet | Liping Ouyang Yingxiao Sun Dan Lv Xiaochun Peng Xiaoming Liu Lei Ci Guoning Zhang Bo Yuan Ling Li Jian Fei Jun Ma Xuanyong Liu Yun Liao |
| author_sort | Liping Ouyang |
| collection | DOAJ |
| description | Summary: Coordination between osteogenesis and angiogenesis is required for bone homeostasis. Here, we show that miR-29cb2 is a bone-specific miRNA and plays critical roles on angiogenesis-osteogenesis coupling during bone remodeling. Mice with deletion of miR-29cb2 exhibit osteopenic phenotypes and osteoblast impairment, accompanied by pronounced decreases in specific H vessels. The decrease in bone miR-29cb2 was associated with pathological ovariectomy stimuli. Mechanistically, hypoxia-inducible factor (HIF)-3α, as a target for miR-29cb2, inhibits HIF-1α activity by competitively bonding with HIF-1β. Notably, miR-29cb2 in peripheral blood (PB) nearly is undetectable in sham and significantly increases in ovariectomy mice. Further evaluation from osteoporosis patients demonstrates similar signatures. ROC analysis shows miR-29cb2 in PB has higher sensitivity and specificity for diagnosing osteoporosis when compared with four clinical biomarkers. Collectively, these findings reveal that miR-29cb2 is essential for bone remodeling by inhibiting HIF-3α and elevated bone-specific miR-29cb2 in PB, which may be a promising biomarker for bone loss |
| first_indexed | 2024-12-23T19:41:26Z |
| format | Article |
| id | doaj.art-f30901f1868c4028bbdcd6bdf488291b |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-23T19:41:26Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-f30901f1868c4028bbdcd6bdf488291b2022-12-21T17:33:40ZengElsevieriScience2589-00422022-01-01251103604miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in boneLiping Ouyang0Yingxiao Sun1Dan Lv2Xiaochun Peng3Xiaoming Liu4Lei Ci5Guoning Zhang6Bo Yuan7Ling Li8Jian Fei9Jun Ma10Xuanyong Liu11Yun Liao12Department of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; Hongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaDepartment of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaDepartment of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaDepartment of Orthopaedics, The Sixth Affiliated People's Hospital, Shanghai Jiaotong University, Shanghai 200233, ChinaDepartment of Orthopedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaShanghai Engineering Research Center for Model Organisms, Shanghai Model Organisms Center, INC., Shanghai 201203, ChinaDepartment of Orthopedics, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaDepartment of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaDepartment of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, ChinaShanghai Engineering Research Center for Model Organisms, Shanghai Model Organisms Center, INC., Shanghai 201203, China; School of Life Science and Technology, Tongji University, Shanghai 200092, ChinaHongqiao International Institute of Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; Corresponding authorState Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; School of Chemistry and Materials Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China; Corresponding authorDepartment of Pharmacy, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200336, China; Corresponding authorSummary: Coordination between osteogenesis and angiogenesis is required for bone homeostasis. Here, we show that miR-29cb2 is a bone-specific miRNA and plays critical roles on angiogenesis-osteogenesis coupling during bone remodeling. Mice with deletion of miR-29cb2 exhibit osteopenic phenotypes and osteoblast impairment, accompanied by pronounced decreases in specific H vessels. The decrease in bone miR-29cb2 was associated with pathological ovariectomy stimuli. Mechanistically, hypoxia-inducible factor (HIF)-3α, as a target for miR-29cb2, inhibits HIF-1α activity by competitively bonding with HIF-1β. Notably, miR-29cb2 in peripheral blood (PB) nearly is undetectable in sham and significantly increases in ovariectomy mice. Further evaluation from osteoporosis patients demonstrates similar signatures. ROC analysis shows miR-29cb2 in PB has higher sensitivity and specificity for diagnosing osteoporosis when compared with four clinical biomarkers. Collectively, these findings reveal that miR-29cb2 is essential for bone remodeling by inhibiting HIF-3α and elevated bone-specific miR-29cb2 in PB, which may be a promising biomarker for bone losshttp://www.sciencedirect.com/science/article/pii/S2589004221015741Molecular biologyDevelopmental biology |
| spellingShingle | Liping Ouyang Yingxiao Sun Dan Lv Xiaochun Peng Xiaoming Liu Lei Ci Guoning Zhang Bo Yuan Ling Li Jian Fei Jun Ma Xuanyong Liu Yun Liao miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone iScience Molecular biology Developmental biology |
| title | miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone |
| title_full | miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone |
| title_fullStr | miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone |
| title_full_unstemmed | miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone |
| title_short | miR-29cb2 promotes angiogenesis and osteogenesis by inhibiting HIF-3α in bone |
| title_sort | mir 29cb2 promotes angiogenesis and osteogenesis by inhibiting hif 3α in bone |
| topic | Molecular biology Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221015741 |
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