Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d
Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Ca...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2019-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-019-10507-3 |
| _version_ | 1818840873512730624 |
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| author | Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang |
| author_facet | Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang |
| author_sort | Bo Zhang |
| collection | DOAJ |
| description | Cas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system. |
| first_indexed | 2024-12-19T04:17:06Z |
| format | Article |
| id | doaj.art-f30e478b0a24490ca6dcfdd16c1b148d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-19T04:17:06Z |
| publishDate | 2019-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f30e478b0a24490ca6dcfdd16c1b148d2022-12-21T20:36:17ZengNature PortfolioNature Communications2041-17232019-06-0110111110.1038/s41467-019-10507-3Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13dBo Zhang0Yangmiao Ye1Weiwei Ye2Vanja Perčulija3Han Jiang4Yiyang Chen5Yu Li6Jing Chen7Jinying Lin8Siqi Wang9Qi Chen10Yu-San Han11Songying Ouyang12The Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityInstitute of Fisheries Science, College of Life Science, National Taiwan UniversityThe Key Laboratory of Innate Immune Biology of Fujian Province, Biomedical Research Center of South China, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, College of Life Sciences, Fujian Normal UniversityCas13d is a class 2 type VI-D CRISPR-Cas RNA-guided RNase. Here the authors present the high-resolution crystal structure of the uncultured Ruminococcus sp. Cas13d (UrCas13d)-crRNA complex and by combining structural, mutational and biochemical studies provide mechanistic insights into the CRISPR-Cas13d system.https://doi.org/10.1038/s41467-019-10507-3 |
| spellingShingle | Bo Zhang Yangmiao Ye Weiwei Ye Vanja Perčulija Han Jiang Yiyang Chen Yu Li Jing Chen Jinying Lin Siqi Wang Qi Chen Yu-San Han Songying Ouyang Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d Nature Communications |
| title | Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d |
| title_full | Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d |
| title_fullStr | Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d |
| title_full_unstemmed | Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d |
| title_short | Two HEPN domains dictate CRISPR RNA maturation and target cleavage in Cas13d |
| title_sort | two hepn domains dictate crispr rna maturation and target cleavage in cas13d |
| url | https://doi.org/10.1038/s41467-019-10507-3 |
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