EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS.
Background: Periodontitis is a chronic inflammatory disruption of the periodontal supportive tissues. There are the numerous evidences for his bacterial etiology. Though the occurrence of periodontal bacteria is considered to be the main cause of periodontitis, certain characteristics of the individ...
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| Format: | Article |
| Language: | English |
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Peytchinski Publishing
2015-09-01
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| Series: | Journal of IMAB |
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| Online Access: | http://www.journal-imab-bg.org/issues-2015/issue3/JofIMAB_2015-21-3p868-875.pdf |
| _version_ | 1818543023156363264 |
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| author | Velitchka Dosseva-Panova Antoaneta Mlachkova Christina Popova Maya Kicheva |
| author_facet | Velitchka Dosseva-Panova Antoaneta Mlachkova Christina Popova Maya Kicheva |
| author_sort | Velitchka Dosseva-Panova |
| collection | DOAJ |
| description | Background: Periodontitis is a chronic inflammatory disruption of the periodontal supportive tissues. There are the numerous evidences for his bacterial etiology. Though the occurrence of periodontal bacteria is considered to be the main cause of periodontitis, certain characteristics of the individual immune response may also have influence on the disease development and progression, and on the treatment outcomes. There are some reports that attempt to identify genetic factors associated with periodontitis including polymorphisms of interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) genes. We were interested from the distribution of several genotypes of the cytokines: interleukin-6 - (G-174C) and (G-597A), lymphotoxin- α (A+252G), and tumour necrosis factor-alpha (G-308A) in patients with chronic periodontitis.
Aim: To investigate the association of chronic periodontitis with certain gene polymorphisms of interleukin-6 (IL-6), Lymphotoxin- α, and tumor necrosis factor-alpha (TNF-α).
Material and methods: The study included 30 patients with moderate or severe chronic periodontitis, and 10 persons with healthy periodontium. Total genomic DNA was extracted from the buccal epithelial cells. TNF-A (G-308A), IL-6 (G-174C), IL-6 (G-597A) and LT-A (A+252G) genes polymorphisms were analyzed by Polymerase chain reaction (PCR).
Results: Outcomes showed a large variety in genotype’s distribution in the investigated groups. No important difference was observed in the distribution of IL-6, TNF-α and LT-α genotypes between chronic periodontitis patients and controls in this study be reason of the small studied group. However, a significant difference in the LT-α was observed – a prevalence of the genotype GG in patients with severe periodontitis. In relation with IL-6 (G-597A) and IL-6 (G-174C) genotyping – in both of them in patients with severe periodontitis was occurred most frequently the genotype GG. In patients with periodontitis the frequency of genotype GG of TNF-α (G-308A) was significantly increased.
Conclusion: The assessment IL-6 (G-597A) and IL-6 (G-174C), and TNF-α (G-308A) revealed that genotype GG was moderate associated with chronic periodontitis in Bulgarian individuals. As a result of these findings we may suppose that the G allele may play an important role in the development and progression of periodontal disease in this population. The frequency of LT-A (A252G) was significantly greater in severe periodontitis patients in this study. |
| first_indexed | 2024-12-11T22:29:51Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1312-773X |
| language | English |
| last_indexed | 2024-12-11T22:29:51Z |
| publishDate | 2015-09-01 |
| publisher | Peytchinski Publishing |
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| series | Journal of IMAB |
| spelling | doaj.art-f30fd857899f481782f5764f72b96c2b2022-12-22T00:48:11ZengPeytchinski PublishingJournal of IMAB1312-773X2015-09-0121386887510.5272/jimab.2015213.868EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS.Velitchka Dosseva-Panova0Antoaneta Mlachkova1Christina Popova2Maya Kicheva3Department of Periodontology, Faculty of Dental Medicine, Medical University of Sofia, BulgariaDepartment of Periodontology, Faculty of Dental Medicine, Medical University of Sofia, BulgariaDepartment of Periodontology, Faculty of Dental Medicine, Medical University of Sofia, BulgariaPhD, biochemist, Progene Ltd., Sofia, Bulgaria.Background: Periodontitis is a chronic inflammatory disruption of the periodontal supportive tissues. There are the numerous evidences for his bacterial etiology. Though the occurrence of periodontal bacteria is considered to be the main cause of periodontitis, certain characteristics of the individual immune response may also have influence on the disease development and progression, and on the treatment outcomes. There are some reports that attempt to identify genetic factors associated with periodontitis including polymorphisms of interleukin-1 beta (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) genes. We were interested from the distribution of several genotypes of the cytokines: interleukin-6 - (G-174C) and (G-597A), lymphotoxin- α (A+252G), and tumour necrosis factor-alpha (G-308A) in patients with chronic periodontitis. Aim: To investigate the association of chronic periodontitis with certain gene polymorphisms of interleukin-6 (IL-6), Lymphotoxin- α, and tumor necrosis factor-alpha (TNF-α). Material and methods: The study included 30 patients with moderate or severe chronic periodontitis, and 10 persons with healthy periodontium. Total genomic DNA was extracted from the buccal epithelial cells. TNF-A (G-308A), IL-6 (G-174C), IL-6 (G-597A) and LT-A (A+252G) genes polymorphisms were analyzed by Polymerase chain reaction (PCR). Results: Outcomes showed a large variety in genotype’s distribution in the investigated groups. No important difference was observed in the distribution of IL-6, TNF-α and LT-α genotypes between chronic periodontitis patients and controls in this study be reason of the small studied group. However, a significant difference in the LT-α was observed – a prevalence of the genotype GG in patients with severe periodontitis. In relation with IL-6 (G-597A) and IL-6 (G-174C) genotyping – in both of them in patients with severe periodontitis was occurred most frequently the genotype GG. In patients with periodontitis the frequency of genotype GG of TNF-α (G-308A) was significantly increased. Conclusion: The assessment IL-6 (G-597A) and IL-6 (G-174C), and TNF-α (G-308A) revealed that genotype GG was moderate associated with chronic periodontitis in Bulgarian individuals. As a result of these findings we may suppose that the G allele may play an important role in the development and progression of periodontal disease in this population. The frequency of LT-A (A252G) was significantly greater in severe periodontitis patients in this study.http://www.journal-imab-bg.org/issues-2015/issue3/JofIMAB_2015-21-3p868-875.pdfgene polymorphisminterleukin-6lymphotoxin-αtumor necrosis factor-alphasusceptibilityperiodontitis |
| spellingShingle | Velitchka Dosseva-Panova Antoaneta Mlachkova Christina Popova Maya Kicheva EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS. Journal of IMAB gene polymorphism interleukin-6 lymphotoxin-α tumor necrosis factor-alpha susceptibility periodontitis |
| title | EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS. |
| title_full | EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS. |
| title_fullStr | EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS. |
| title_full_unstemmed | EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS. |
| title_short | EVALUATION OF INTERLEUKIN-6, LYMPHOTOXIN-α AND TNF-α GENE POLYMORPHISMS IN CHRONIC PERIODONTITIS. |
| title_sort | evaluation of interleukin 6 lymphotoxin α and tnf α gene polymorphisms in chronic periodontitis |
| topic | gene polymorphism interleukin-6 lymphotoxin-α tumor necrosis factor-alpha susceptibility periodontitis |
| url | http://www.journal-imab-bg.org/issues-2015/issue3/JofIMAB_2015-21-3p868-875.pdf |
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