Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits
This study aimed to evaluate the in vitro antimicrobial activities of two types of insect fats extracted from black soldier fly larvae (HI, <i>Hermetia illucens</i> L.) and yellow mealworm larvae (TM, <i>Tenebrio molitor</i> L.) and their effects as dietary replacement of soy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/8/1292 |
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| author | Sihem Dabbou Ilario Ferrocino Laura Gasco Achille Schiavone Angela Trocino Gerolamo Xiccato Ana C. Barroeta Sandra Maione Dominga Soglia Ilaria Biasato Luca Cocolin Francesco Gai Daniele Michele Nucera |
| author_facet | Sihem Dabbou Ilario Ferrocino Laura Gasco Achille Schiavone Angela Trocino Gerolamo Xiccato Ana C. Barroeta Sandra Maione Dominga Soglia Ilaria Biasato Luca Cocolin Francesco Gai Daniele Michele Nucera |
| author_sort | Sihem Dabbou |
| collection | DOAJ |
| description | This study aimed to evaluate the in vitro antimicrobial activities of two types of insect fats extracted from black soldier fly larvae (HI, <i>Hermetia illucens</i> L.) and yellow mealworm larvae (TM, <i>Tenebrio molitor</i> L.) and their effects as dietary replacement of soybean oil (S) on cecal fermentation pattern, and fecal and cecal microbiota in rabbits. A total of 120 weaned rabbits were randomly allotted to three dietary treatments (40 rabbits/group) —a control diet (C diet) containing 1.5% of S and two experimental diets (HI diet (HID) and TM diet (TMD)), where S was totally substituted by HI or TM fats during the whole trial that lasted 41 days. Regarding the in vitro antimicrobial activities, HI and TM fats did not show any effects on <i>Salmonella</i> growth. <i>Yersinia enterocolitica</i> showed significantly lower growth when challenged with HI fats than the controls. The insect fat supplementation in rabbit diets increased the contents of the cecal volatile fatty acids when compared to the control group. A metataxonomic approach was adopted to investigate the shift in the microbial composition as a function of the dietary insect fat supplementation. The microbiota did not show a clear separation as a function of the inclusion, even if a specific microbial signature was observed. Indeed, HI and TM fat supplementation enriched the presence of <i>Akkermansia</i> that was found to be correlated with NH3-N concentration. An increase in <i>Ruminococcus</i>, which can improve the immune response of the host, was also observed. This study confirms the potential of HI and TM fats as antibacterial feed ingredients with a positive influence on the rabbit cecal microbiota, thus supporting the possibility of including HI and TM fats in rabbit diets. |
| first_indexed | 2024-03-10T18:09:37Z |
| format | Article |
| id | doaj.art-f30fe8add2ab4e5b8eb6bcdd8d7b79d4 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T18:09:37Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-f30fe8add2ab4e5b8eb6bcdd8d7b79d42023-11-20T08:15:14ZengMDPI AGAnimals2076-26152020-07-01108129210.3390/ani10081292Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing RabbitsSihem Dabbou0Ilario Ferrocino1Laura Gasco2Achille Schiavone3Angela Trocino4Gerolamo Xiccato5Ana C. Barroeta6Sandra Maione7Dominga Soglia8Ilaria Biasato9Luca Cocolin10Francesco Gai11Daniele Michele Nucera12Center Agriculture Food Environment (C3A), University of Trento, Via E. Mach 1, 38010 San Michele all’Adige, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, ItalyDepartment of Comparative Biomedicine and Food Science, University of Padova, Viale dell’Università 16, Legnaro, 35020 Padova, ItalyDepartment of Agronomy, Food, Natural Resources, Animal, and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro, Padova, ItalyNutrition and Animal Welfare Service, Department of Animal and Food Science, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, 08193 Barcelona, SpainDepartment of Veterinary Science, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Turin, Largo P. Braccini 2, 10095 Grugliasco, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyInstitute of Sciences of Food Production, National Research Council, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Agricultural, Forest and Food Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyThis study aimed to evaluate the in vitro antimicrobial activities of two types of insect fats extracted from black soldier fly larvae (HI, <i>Hermetia illucens</i> L.) and yellow mealworm larvae (TM, <i>Tenebrio molitor</i> L.) and their effects as dietary replacement of soybean oil (S) on cecal fermentation pattern, and fecal and cecal microbiota in rabbits. A total of 120 weaned rabbits were randomly allotted to three dietary treatments (40 rabbits/group) —a control diet (C diet) containing 1.5% of S and two experimental diets (HI diet (HID) and TM diet (TMD)), where S was totally substituted by HI or TM fats during the whole trial that lasted 41 days. Regarding the in vitro antimicrobial activities, HI and TM fats did not show any effects on <i>Salmonella</i> growth. <i>Yersinia enterocolitica</i> showed significantly lower growth when challenged with HI fats than the controls. The insect fat supplementation in rabbit diets increased the contents of the cecal volatile fatty acids when compared to the control group. A metataxonomic approach was adopted to investigate the shift in the microbial composition as a function of the dietary insect fat supplementation. The microbiota did not show a clear separation as a function of the inclusion, even if a specific microbial signature was observed. Indeed, HI and TM fat supplementation enriched the presence of <i>Akkermansia</i> that was found to be correlated with NH3-N concentration. An increase in <i>Ruminococcus</i>, which can improve the immune response of the host, was also observed. This study confirms the potential of HI and TM fats as antibacterial feed ingredients with a positive influence on the rabbit cecal microbiota, thus supporting the possibility of including HI and TM fats in rabbit diets.https://www.mdpi.com/2076-2615/10/8/1292insect fat<i>Hermetia illucens</i><i>Tenebrio molitor</i>gut microbiotaantimicrobial effectrabbit feeding |
| spellingShingle | Sihem Dabbou Ilario Ferrocino Laura Gasco Achille Schiavone Angela Trocino Gerolamo Xiccato Ana C. Barroeta Sandra Maione Dominga Soglia Ilaria Biasato Luca Cocolin Francesco Gai Daniele Michele Nucera Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits Animals insect fat <i>Hermetia illucens</i> <i>Tenebrio molitor</i> gut microbiota antimicrobial effect rabbit feeding |
| title | Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits |
| title_full | Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits |
| title_fullStr | Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits |
| title_full_unstemmed | Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits |
| title_short | Antimicrobial Effects of Black Soldier Fly and Yellow Mealworm Fats and Their Impact on Gut Microbiota of Growing Rabbits |
| title_sort | antimicrobial effects of black soldier fly and yellow mealworm fats and their impact on gut microbiota of growing rabbits |
| topic | insect fat <i>Hermetia illucens</i> <i>Tenebrio molitor</i> gut microbiota antimicrobial effect rabbit feeding |
| url | https://www.mdpi.com/2076-2615/10/8/1292 |
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