ATP changes the fluorescence lifetime of cyan fluorescent protein via an interaction with His148.

Recently, we described that ATP induces changes in YFP/CFP fluorescence intensities of Fluorescence Resonance Energy Transfer (FRET) sensors based on CFP-YFP. To get insight into this phenomenon, we employed fluorescence lifetime spectroscopy to analyze the influence of ATP on these fluorescent prot...

Full description

Bibliographic Details
Main Authors: Jan Willem Borst, Marieke Willemse, Rik Slijkhuis, Gerard van der Krogt, Sergey P Laptenok, Kees Jalink, Be Wieringa, Jack A M Fransen
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2010-11-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC2974648?pdf=render