Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes
AIM: To construct recombination eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes. <p>METHODS:We amplified the target gene of shuttle vector PHMCMVTSHR289, conjugated the target gene and eukaryotic expression plasmid pcDNA3.1+...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-12-01
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| Series: | Guoji Yanke Zazhi |
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| Online Access: | http://ies.ijo.cn/cn_publish/2014/12/201412011.pdf |
| _version_ | 1818548413205053440 |
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| author | Yu-Li Yang Qing-Li Luo Hong-Bin Lü |
| author_facet | Yu-Li Yang Qing-Li Luo Hong-Bin Lü |
| author_sort | Yu-Li Yang |
| collection | DOAJ |
| description | AIM: To construct recombination eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes. <p>METHODS:We amplified the target gene of shuttle vector PHMCMVTSHR289, conjugated the target gene and eukaryotic expression plasmid pcDNA3.1+, and accredited whether pcDNA3.1+/TSHR289 was connected or not by enzymatic digestion and sequencing. Cationic liposomes encapsulated the recombination plasmid pcDNA3.1+/TSHR289.<p>RESULTS: Recombination plasmid pcDNA3.1+/TSHR289 digested with enzyme HindIII and the fragment through 0.8% gel electrophoresis showed 512bp strip. Recombination plasmid pcDNA3.1+/TSHR289 were found synonymous mutation through forward(AAC to AAT)and reverse sequencing(GCG to GCT). The volume ratio of cationic liposomes and recombinant plasmid was 3:1. <p>CONCLUSION: It is successful to construct the recombination plasmid pcDNA3.1+/TSHR289 by accredit it through enzymatic digestion and sequencing. |
| first_indexed | 2024-12-12T08:19:35Z |
| format | Article |
| id | doaj.art-f3160b8067394744befb882e9ff2071c |
| institution | Directory Open Access Journal |
| issn | 1672-5123 1672-5123 |
| language | English |
| last_indexed | 2024-12-12T08:19:35Z |
| publishDate | 2014-12-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | Guoji Yanke Zazhi |
| spelling | doaj.art-f3160b8067394744befb882e9ff2071c2022-12-22T00:31:28ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232014-12-0114122151215410.3980/j.issn.1672-5123.2014.12.11Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomesYu-Li Yang0Qing-Li Luo1Hong-Bin Lü2Southwest Eye Hospital, Southwest Hospital, Third Military Medical University, Chongqing 400038,ChinaDepartment of Ophthalmology, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, ChinaDepartment of Ophthalmology, Affiliated Hospital of Luzhou Medical University,Luzhou 646000,Sichuan Province, ChinaAIM: To construct recombination eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes. <p>METHODS:We amplified the target gene of shuttle vector PHMCMVTSHR289, conjugated the target gene and eukaryotic expression plasmid pcDNA3.1+, and accredited whether pcDNA3.1+/TSHR289 was connected or not by enzymatic digestion and sequencing. Cationic liposomes encapsulated the recombination plasmid pcDNA3.1+/TSHR289.<p>RESULTS: Recombination plasmid pcDNA3.1+/TSHR289 digested with enzyme HindIII and the fragment through 0.8% gel electrophoresis showed 512bp strip. Recombination plasmid pcDNA3.1+/TSHR289 were found synonymous mutation through forward(AAC to AAT)and reverse sequencing(GCG to GCT). The volume ratio of cationic liposomes and recombinant plasmid was 3:1. <p>CONCLUSION: It is successful to construct the recombination plasmid pcDNA3.1+/TSHR289 by accredit it through enzymatic digestion and sequencing.http://ies.ijo.cn/cn_publish/2014/12/201412011.pdfhuman thyrotropin receptor extracellular domain geneshuttle vector PHMCMVTSHR289eukaryotic expression plasmid pcDNA3.1+recombination plasmid pcDNA3.1+/TSHR289cationic liposomes |
| spellingShingle | Yu-Li Yang Qing-Li Luo Hong-Bin Lü Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes Guoji Yanke Zazhi human thyrotropin receptor extracellular domain gene shuttle vector PHMCMVTSHR289 eukaryotic expression plasmid pcDNA3.1+ recombination plasmid pcDNA3.1+/TSHR289 cationic liposomes |
| title | Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes |
| title_full | Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes |
| title_fullStr | Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes |
| title_full_unstemmed | Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes |
| title_short | Construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes |
| title_sort | construction of recombinant eukaryotic expression plasmid of human thyrotropin receptor extracellular domain encapsulated with cationic liposomes |
| topic | human thyrotropin receptor extracellular domain gene shuttle vector PHMCMVTSHR289 eukaryotic expression plasmid pcDNA3.1+ recombination plasmid pcDNA3.1+/TSHR289 cationic liposomes |
| url | http://ies.ijo.cn/cn_publish/2014/12/201412011.pdf |
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