Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources
This article presents a study of D-loop sequences to characterize the genetic diversity of wild Macrobrachium nipponense populations in Yixing natural waters including two reservoirs (Hengshan reservoir, HS; Youche reservoir, YC), 3 brooks (Linjin Dang, LJD; Magong Dushan Dang, MDD; Yangshan Dang, Y...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2023-01-01
|
| Series: | Aquatic Living Resources |
| Subjects: | |
| Online Access: | https://www.alr-journal.org/articles/alr/full_html/2023/01/alr220052/alr220052.html |
| _version_ | 1827828434121785344 |
|---|---|
| author | Xiong Yiwei Jiang Sufei Zhang Lijuan Wang Jisheng Zhang Wenyi Jin Shubo Gong Yongsheng Wu Yan Qiao Hui Fu Hongtuo |
| author_facet | Xiong Yiwei Jiang Sufei Zhang Lijuan Wang Jisheng Zhang Wenyi Jin Shubo Gong Yongsheng Wu Yan Qiao Hui Fu Hongtuo |
| author_sort | Xiong Yiwei |
| collection | DOAJ |
| description | This article presents a study of D-loop sequences to characterize the genetic diversity of wild Macrobrachium nipponense populations in Yixing natural waters including two reservoirs (Hengshan reservoir, HS; Youche reservoir, YC), 3 brooks (Linjin Dang, LJD; Magong Dushan Dang, MDD; Yangshan Dang, YSD) and 3 shallow lakes (Dongjiu lake, DJ; Xijiu lake, XJ; Tuanjiu lake, XJ), and compared the genetic differentiation and population structure with wild populations of Taihu Lake (TH), Yangtze River (YZ), and the main local artificially bred varieties “Taihu No. 2” (TH-2). A 747 bp D-loop sequence fragment was amplified in 321 individuals and the results exhibited a higher content of A+T (80.03%) than C+G (19.97%). A total of 110 haplotypes were identified. The h and π value proved the diversity of these populations was at the same level with high genetic diversity. TH-2 and YZ showed remarkable diversity, and XJ is even better. Fst estimates suggested that YZ and TH-2 were significant differentiation with other Yixing populations (P < 0.05). Three populations from shallow lake (DJ, XJ and TJ) displayed significant differentiated with the left Yixing ones (P < 0.05). The pairwise genetic distance, as well as haplotype network results, also suggested that all these 11 populations did not diverge at the species level (<15%). The P values of Tajima's D and Fu Fs were relatively greater than 0.1 (P > 0.1) and the nucleotide mismatch distribution analysis showed multiple peaks, giving a conclusion that the populations did not exhibited expansion. All these results suggested that TH-2 and YZ have remarkable diversity, and the germplasm resources and genetic diversity of M. nipponense in Yixing are very good and are suitable for original materials of breeding. |
| first_indexed | 2024-03-12T03:51:06Z |
| format | Article |
| id | doaj.art-f316ef7fd41b4f27a212e5f556dce3db |
| institution | Directory Open Access Journal |
| issn | 1765-2952 |
| language | English |
| last_indexed | 2024-03-12T03:51:06Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Aquatic Living Resources |
| spelling | doaj.art-f316ef7fd41b4f27a212e5f556dce3db2023-09-03T12:21:33ZengEDP SciencesAquatic Living Resources1765-29522023-01-0136510.1051/alr/2023002alr220052Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resourcesXiong Yiwei0Jiang Sufei1Zhang Lijuan2Wang Jisheng3Zhang Wenyi4Jin Shubo5Gong Yongsheng6Wu Yan7Qiao Hui8https://orcid.org/0000-0002-9242-2205Fu Hongtuo9Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesWuxi Fisheries College, Nanjing Agricultural UniversityWuxi Fisheries College, Nanjing Agricultural UniversityKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery SciencesThis article presents a study of D-loop sequences to characterize the genetic diversity of wild Macrobrachium nipponense populations in Yixing natural waters including two reservoirs (Hengshan reservoir, HS; Youche reservoir, YC), 3 brooks (Linjin Dang, LJD; Magong Dushan Dang, MDD; Yangshan Dang, YSD) and 3 shallow lakes (Dongjiu lake, DJ; Xijiu lake, XJ; Tuanjiu lake, XJ), and compared the genetic differentiation and population structure with wild populations of Taihu Lake (TH), Yangtze River (YZ), and the main local artificially bred varieties “Taihu No. 2” (TH-2). A 747 bp D-loop sequence fragment was amplified in 321 individuals and the results exhibited a higher content of A+T (80.03%) than C+G (19.97%). A total of 110 haplotypes were identified. The h and π value proved the diversity of these populations was at the same level with high genetic diversity. TH-2 and YZ showed remarkable diversity, and XJ is even better. Fst estimates suggested that YZ and TH-2 were significant differentiation with other Yixing populations (P < 0.05). Three populations from shallow lake (DJ, XJ and TJ) displayed significant differentiated with the left Yixing ones (P < 0.05). The pairwise genetic distance, as well as haplotype network results, also suggested that all these 11 populations did not diverge at the species level (<15%). The P values of Tajima's D and Fu Fs were relatively greater than 0.1 (P > 0.1) and the nucleotide mismatch distribution analysis showed multiple peaks, giving a conclusion that the populations did not exhibited expansion. All these results suggested that TH-2 and YZ have remarkable diversity, and the germplasm resources and genetic diversity of M. nipponense in Yixing are very good and are suitable for original materials of breeding.https://www.alr-journal.org/articles/alr/full_html/2023/01/alr220052/alr220052.htmlmacrobrachium nipponensemitochondrial dna d-loopgenetic diversitygenetic structure |
| spellingShingle | Xiong Yiwei Jiang Sufei Zhang Lijuan Wang Jisheng Zhang Wenyi Jin Shubo Gong Yongsheng Wu Yan Qiao Hui Fu Hongtuo Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources Aquatic Living Resources macrobrachium nipponense mitochondrial dna d-loop genetic diversity genetic structure |
| title | Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources |
| title_full | Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources |
| title_fullStr | Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources |
| title_full_unstemmed | Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources |
| title_short | Genetic diversity and structure assessment of Macrobrachium nipponense populations: implications for the protection and management of genetic resources |
| title_sort | genetic diversity and structure assessment of macrobrachium nipponense populations implications for the protection and management of genetic resources |
| topic | macrobrachium nipponense mitochondrial dna d-loop genetic diversity genetic structure |
| url | https://www.alr-journal.org/articles/alr/full_html/2023/01/alr220052/alr220052.html |
| work_keys_str_mv | AT xiongyiwei geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT jiangsufei geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT zhanglijuan geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT wangjisheng geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT zhangwenyi geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT jinshubo geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT gongyongsheng geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT wuyan geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT qiaohui geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources AT fuhongtuo geneticdiversityandstructureassessmentofmacrobrachiumnipponensepopulationsimplicationsfortheprotectionandmanagementofgeneticresources |