Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency
Multivesicular body (MVB)-mediated endosomal sorting and macroautophagy are the main pathways mediating the transport of cellular components to the vacuole and are essential for maintaining cellular homeostasis. The interplay of these two pathways remains poorly understood in plants. In this study,...
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MDPI AG
2021-08-01
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| Series: | International Journal of Molecular Sciences |
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| author | Tianrui Zhang Zhidan Xiao Chuanliang Liu Chao Yang Jiayi Li Hongbo Li Caiji Gao Wenjin Shen |
| author_facet | Tianrui Zhang Zhidan Xiao Chuanliang Liu Chao Yang Jiayi Li Hongbo Li Caiji Gao Wenjin Shen |
| author_sort | Tianrui Zhang |
| collection | DOAJ |
| description | Multivesicular body (MVB)-mediated endosomal sorting and macroautophagy are the main pathways mediating the transport of cellular components to the vacuole and are essential for maintaining cellular homeostasis. The interplay of these two pathways remains poorly understood in plants. In this study, we show that FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT), essential for MVB biogenesis and plant growth, can be transported to the vacuole for degradation in response to iron deficiency. The vacuolar transport of ubiquitinated FREE1 protein is mediated by the autophagy pathway. As a consequence, the autophagy deficient mutants, <i>atg5-1</i> and <i>atg7-2</i>, accumulate more endogenous FREE1 protein and display hypersensitivity to iron deficiency. Furthermore, under iron-deficient growth condition autophagy related genes are upregulated to promote the autophagic degradation of FREE1, thereby possibly relieving the repressive effect of FREE1 on iron absorption. Collectively, our findings demonstrate a unique regulatory mode of protein turnover of the ESCRT machinery through the autophagy pathway to respond to iron deficiency in plants. |
| first_indexed | 2024-03-10T08:45:20Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T08:45:20Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f316ff723cff4b40b56bbc2f62df4c7f2023-11-22T08:00:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-012216877910.3390/ijms22168779Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron DeficiencyTianrui Zhang0Zhidan Xiao1Chuanliang Liu2Chao Yang3Jiayi Li4Hongbo Li5Caiji Gao6Wenjin Shen7Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaKey Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of Tropical and Subtropical Fruit Tree Research, Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaGuangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, ChinaMultivesicular body (MVB)-mediated endosomal sorting and macroautophagy are the main pathways mediating the transport of cellular components to the vacuole and are essential for maintaining cellular homeostasis. The interplay of these two pathways remains poorly understood in plants. In this study, we show that FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant-specific component of the endosomal sorting complex required for transport (ESCRT), essential for MVB biogenesis and plant growth, can be transported to the vacuole for degradation in response to iron deficiency. The vacuolar transport of ubiquitinated FREE1 protein is mediated by the autophagy pathway. As a consequence, the autophagy deficient mutants, <i>atg5-1</i> and <i>atg7-2</i>, accumulate more endogenous FREE1 protein and display hypersensitivity to iron deficiency. Furthermore, under iron-deficient growth condition autophagy related genes are upregulated to promote the autophagic degradation of FREE1, thereby possibly relieving the repressive effect of FREE1 on iron absorption. Collectively, our findings demonstrate a unique regulatory mode of protein turnover of the ESCRT machinery through the autophagy pathway to respond to iron deficiency in plants.https://www.mdpi.com/1422-0067/22/16/8779protein degradationautophagyESCRT complexFREE1iron-deficiency |
| spellingShingle | Tianrui Zhang Zhidan Xiao Chuanliang Liu Chao Yang Jiayi Li Hongbo Li Caiji Gao Wenjin Shen Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency International Journal of Molecular Sciences protein degradation autophagy ESCRT complex FREE1 iron-deficiency |
| title | Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency |
| title_full | Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency |
| title_fullStr | Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency |
| title_full_unstemmed | Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency |
| title_short | Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency |
| title_sort | autophagy mediates the degradation of plant escrt component free1 in response to iron deficiency |
| topic | protein degradation autophagy ESCRT complex FREE1 iron-deficiency |
| url | https://www.mdpi.com/1422-0067/22/16/8779 |
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