Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution
It is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion o...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-05-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2297-8739/5/2/29 |
| _version_ | 1798026676073922560 |
|---|---|
| author | Yuri D. Ivanov Andrey F. Kozlov Rafael A. Galiullin Vadim Yu. Tatur Vadim S. Ziborov Sergey A. Usanov Tatyana O. Pleshakova |
| author_facet | Yuri D. Ivanov Andrey F. Kozlov Rafael A. Galiullin Vadim Yu. Tatur Vadim S. Ziborov Sergey A. Usanov Tatyana O. Pleshakova |
| author_sort | Yuri D. Ivanov |
| collection | DOAJ |
| description | It is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion of the analyzed solution. Earlier, we demonstrated that a pulsed electric field can well be used for the enhancement of the efficiency of AFM-based fishing of low-abundant protein. In this paper, we have demonstrated the influence of a pulsed electric field on the stimulation of the electric charge generation in a solution of low-abundant proteins observed in the injector part of an AFM-based fishing system at 23 °C and 38 °C. Taking this effect into account is important for the development of novel highly sensitive flow-based diagnostic systems, as well as for the development of models describing the influence of a pulsed electric field on pathological processes in the body, hemodynamics, and physicochemical properties of solutions. |
| first_indexed | 2024-04-11T18:40:16Z |
| format | Article |
| id | doaj.art-f3170187494e4bb6b57084302f0a20ca |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-11T18:40:16Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-f3170187494e4bb6b57084302f0a20ca2022-12-22T04:09:03ZengMDPI AGSeparations2297-87392018-05-01522910.3390/separations5020029separations5020029Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein SolutionYuri D. Ivanov0Andrey F. Kozlov1Rafael A. Galiullin2Vadim Yu. Tatur3Vadim S. Ziborov4Sergey A. Usanov5Tatyana O. Pleshakova6Institute of Biomedical Chemistry, Pogodinskaya st., 10, Moscow 119121, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, Moscow 119121, RussiaInstitute of Biomedical Chemistry, Pogodinskaya st., 10, Moscow 119121, RussiaFoundation of Perspective Technologies and Novations, Moscow 115682, RussiaJoint Institute for High Temperatures of the Russian Academy of Sciences, Moscow 125412, RussiaInstitute of Bioorganic Chemistry, National Academy of Sciences of Belarus, Minsk 220141, BelarusInstitute of Biomedical Chemistry, Pogodinskaya st., 10, Moscow 119121, RussiaIt is known that a charge is generated in water and protein solutions upon their motion; this phenomenon is also observed in analytical systems for atomic force microscopy (AFM)-based fishing. At that, the efficiency of protein fishing correlates with the value of charge, generated upon the motion of the analyzed solution. Earlier, we demonstrated that a pulsed electric field can well be used for the enhancement of the efficiency of AFM-based fishing of low-abundant protein. In this paper, we have demonstrated the influence of a pulsed electric field on the stimulation of the electric charge generation in a solution of low-abundant proteins observed in the injector part of an AFM-based fishing system at 23 °C and 38 °C. Taking this effect into account is important for the development of novel highly sensitive flow-based diagnostic systems, as well as for the development of models describing the influence of a pulsed electric field on pathological processes in the body, hemodynamics, and physicochemical properties of solutions.http://www.mdpi.com/2297-8739/5/2/29analytical flow-based systemsflowing protein solutioncharge accumulation |
| spellingShingle | Yuri D. Ivanov Andrey F. Kozlov Rafael A. Galiullin Vadim Yu. Tatur Vadim S. Ziborov Sergey A. Usanov Tatyana O. Pleshakova Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution Separations analytical flow-based systems flowing protein solution charge accumulation |
| title | Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution |
| title_full | Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution |
| title_fullStr | Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution |
| title_full_unstemmed | Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution |
| title_short | Influence of a Pulsed Electric Field on Charge Generation in a Flowing Protein Solution |
| title_sort | influence of a pulsed electric field on charge generation in a flowing protein solution |
| topic | analytical flow-based systems flowing protein solution charge accumulation |
| url | http://www.mdpi.com/2297-8739/5/2/29 |
| work_keys_str_mv | AT yuridivanov influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution AT andreyfkozlov influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution AT rafaelagaliullin influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution AT vadimyutatur influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution AT vadimsziborov influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution AT sergeyausanov influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution AT tatyanaopleshakova influenceofapulsedelectricfieldonchargegenerationinaflowingproteinsolution |