RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i>
The instability of messenger RNA is crucial to the control of gene expression. In <i>Bacillus subtilis</i>, RNase Y is the major decay-initiating endoribonuclease. Here, we show how this key enzyme regulates its own synthesis by modulating the longevity of its mRNA. Autoregulation is ach...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1374 |
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| author | Anna Korobeinikova Soumaya Laalami Clément Berthy Harald Putzer |
| author_facet | Anna Korobeinikova Soumaya Laalami Clément Berthy Harald Putzer |
| author_sort | Anna Korobeinikova |
| collection | DOAJ |
| description | The instability of messenger RNA is crucial to the control of gene expression. In <i>Bacillus subtilis</i>, RNase Y is the major decay-initiating endoribonuclease. Here, we show how this key enzyme regulates its own synthesis by modulating the longevity of its mRNA. Autoregulation is achieved through cleavages in two regions of the <i>rny</i> (RNase Y) transcript: (i) within the first ~100 nucleotides of the open reading frame, immediately inactivating the mRNA for further rounds of translation; (ii) cleavages in the <i>rny</i> 5′ UTR, primarily within the 5′-terminal 50 nucleotides, creating entry sites for the 5′ exonuclease J1 whose progression is blocked around position −15 of the <i>rny</i> mRNA, potentially by initiating ribosomes. This links the functional inactivation of the transcript by RNase J1 to translation efficiency, depending on the ribosome occupancy at the translation initiation site. By these mechanisms, RNase Y can initiate degradation of its own mRNA when the enzyme is not occupied with degradation of other RNAs and thus prevent its overexpression beyond the needs of RNA metabolism. |
| first_indexed | 2024-03-11T02:08:49Z |
| format | Article |
| id | doaj.art-f3172aa19a9e4f76b132ae352728483d |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T02:08:49Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-f3172aa19a9e4f76b132ae352728483d2023-11-18T11:41:01ZengMDPI AGMicroorganisms2076-26072023-05-01116137410.3390/microorganisms11061374RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i>Anna Korobeinikova0Soumaya Laalami1Clément Berthy2Harald Putzer3Expression Génétique Microbienne, Institut de Biologie Physico-Chimique, CNRS, Université Paris Cité, 75005 Paris, FranceExpression Génétique Microbienne, Institut de Biologie Physico-Chimique, CNRS, Université Paris Cité, 75005 Paris, FranceExpression Génétique Microbienne, Institut de Biologie Physico-Chimique, CNRS, Université Paris Cité, 75005 Paris, FranceExpression Génétique Microbienne, Institut de Biologie Physico-Chimique, CNRS, Université Paris Cité, 75005 Paris, FranceThe instability of messenger RNA is crucial to the control of gene expression. In <i>Bacillus subtilis</i>, RNase Y is the major decay-initiating endoribonuclease. Here, we show how this key enzyme regulates its own synthesis by modulating the longevity of its mRNA. Autoregulation is achieved through cleavages in two regions of the <i>rny</i> (RNase Y) transcript: (i) within the first ~100 nucleotides of the open reading frame, immediately inactivating the mRNA for further rounds of translation; (ii) cleavages in the <i>rny</i> 5′ UTR, primarily within the 5′-terminal 50 nucleotides, creating entry sites for the 5′ exonuclease J1 whose progression is blocked around position −15 of the <i>rny</i> mRNA, potentially by initiating ribosomes. This links the functional inactivation of the transcript by RNase J1 to translation efficiency, depending on the ribosome occupancy at the translation initiation site. By these mechanisms, RNase Y can initiate degradation of its own mRNA when the enzyme is not occupied with degradation of other RNAs and thus prevent its overexpression beyond the needs of RNA metabolism.https://www.mdpi.com/2076-2607/11/6/1374RNase YmRNA degradationbacillus subtilisribonuclease |
| spellingShingle | Anna Korobeinikova Soumaya Laalami Clément Berthy Harald Putzer RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i> Microorganisms RNase Y mRNA degradation bacillus subtilis ribonuclease |
| title | RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i> |
| title_full | RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i> |
| title_fullStr | RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i> |
| title_full_unstemmed | RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i> |
| title_short | RNase Y Autoregulates Its Synthesis in <i>Bacillus subtilis</i> |
| title_sort | rnase y autoregulates its synthesis in i bacillus subtilis i |
| topic | RNase Y mRNA degradation bacillus subtilis ribonuclease |
| url | https://www.mdpi.com/2076-2607/11/6/1374 |
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