<i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene
<i>Bracon brevicornis</i> is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, <i>Ostrinia nubilalis</i>. It is also one of the earliest documented cases of complementary sex determination in H...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/12/1390 |
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| author | Kim B. Ferguson Bart A. Pannebakker Alejandra Centurión Joost van den Heuvel Ronald Nieuwenhuis Frank F. M. Becker Elio Schijlen Andra Thiel Bas J. Zwaan Eveline C. Verhulst |
| author_facet | Kim B. Ferguson Bart A. Pannebakker Alejandra Centurión Joost van den Heuvel Ronald Nieuwenhuis Frank F. M. Becker Elio Schijlen Andra Thiel Bas J. Zwaan Eveline C. Verhulst |
| author_sort | Kim B. Ferguson |
| collection | DOAJ |
| description | <i>Bracon brevicornis</i> is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, <i>Ostrinia nubilalis</i>. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of <i>B. brevicornis</i>, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, <i>Fopius arisanus</i> and <i>Diachasma alloeum</i>. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the <i>complementary sex determiner</i> gene (<i>csd</i>). |
| first_indexed | 2024-03-10T14:37:26Z |
| format | Article |
| id | doaj.art-f3172e7f51954fd085b987f0e2177626 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T14:37:26Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-f3172e7f51954fd085b987f0e21776262023-11-20T22:04:23ZengMDPI AGGenes2073-44252020-11-011112139010.3390/genes11121390<i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> GeneKim B. Ferguson0Bart A. Pannebakker1Alejandra Centurión2Joost van den Heuvel3Ronald Nieuwenhuis4Frank F. M. Becker5Elio Schijlen6Andra Thiel7Bas J. Zwaan8Eveline C. Verhulst9Laboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The NetherlandsLaboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The NetherlandsPopulation and Evolutionary Ecology Group, Institute of Ecology, FB02, University of Bremen, 28359 Bremen, GermanyLaboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The NetherlandsBioscience, Wageningen University & Research, 6708PB Wageningen, The NetherlandsLaboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The NetherlandsBioscience, Wageningen University & Research, 6708PB Wageningen, The NetherlandsPopulation and Evolutionary Ecology Group, Institute of Ecology, FB02, University of Bremen, 28359 Bremen, GermanyLaboratory of Genetics, Wageningen University & Research, 6708PB Wageningen, The NetherlandsLaboratory of Entomology, Wageningen University & Research, 6708PB Wageningen, The Netherlands<i>Bracon brevicornis</i> is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, <i>Ostrinia nubilalis</i>. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read-based genome of <i>B. brevicornis</i>, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, <i>Fopius arisanus</i> and <i>Diachasma alloeum</i>. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the <i>complementary sex determiner</i> gene (<i>csd</i>).https://www.mdpi.com/2073-4425/11/12/139010X GenomicsBraconinaecomplementary sex determinationmicrosynteny<i>feminizer</i>parasitoid |
| spellingShingle | Kim B. Ferguson Bart A. Pannebakker Alejandra Centurión Joost van den Heuvel Ronald Nieuwenhuis Frank F. M. Becker Elio Schijlen Andra Thiel Bas J. Zwaan Eveline C. Verhulst <i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene Genes 10X Genomics Braconinae complementary sex determination microsynteny <i>feminizer</i> parasitoid |
| title | <i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene |
| title_full | <i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene |
| title_fullStr | <i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene |
| title_full_unstemmed | <i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene |
| title_short | <i>Bracon brevicornis</i> Genome Showcases the Potential of Linked-Read Sequencing in Identifying a Putative <i>Complementary Sex Determiner</i> Gene |
| title_sort | i bracon brevicornis i genome showcases the potential of linked read sequencing in identifying a putative i complementary sex determiner i gene |
| topic | 10X Genomics Braconinae complementary sex determination microsynteny <i>feminizer</i> parasitoid |
| url | https://www.mdpi.com/2073-4425/11/12/1390 |
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