Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003
A practical medium for reducing costs and enhancing both biomass and intracellular polysaccharide (IPS) production by a newly screened Agaricus blazei AB003 has been selected. The results show that IPS production was growth-associated, that a combination of corn flour extract and glucose was the bes...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2009-01-01
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| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/59887 |
| Summary: | A practical medium for reducing costs and enhancing both biomass and intracellular polysaccharide (IPS) production by a newly screened Agaricus blazei AB003 has been selected. The results show that IPS production was growth-associated, that a combination of corn flour extract and glucose was the best carbon source, and that a combination of wheat bran extract and yeast extract was the best nitrogen source for both biomass and IPS production. The effects of the four factors were further investigated using four-factor, three-level orthogonal test design. The best combination for the production of biomass and IPS was (in g/L): corn flour extract 15, glucose 6, yeast extract 3, and wheat bran extract 6. The maximum biomass and IPS in a 30-litre stirred tank bioreactor reached 11.30 and 0.72 g/L in the optimized medium, respectively. The use of two low-cost raw materials like corn flour and wheat bran extract as the major medium constituents could reduce the costs of biomass and IPS production. |
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| ISSN: | 1330-9862 1334-2606 |