Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003
A practical medium for reducing costs and enhancing both biomass and intracellular polysaccharide (IPS) production by a newly screened Agaricus blazei AB003 has been selected. The results show that IPS production was growth-associated, that a combination of corn flour extract and glucose was the bes...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Zagreb Faculty of Food Technology and Biotechnology
2009-01-01
|
| Series: | Food Technology and Biotechnology |
| Subjects: | |
| Online Access: | http://hrcak.srce.hr/file/59887 |
| _version_ | 1797430226597183488 |
|---|---|
| author | Xiao-Ling Wang Gao-Qiang Liu |
| author_facet | Xiao-Ling Wang Gao-Qiang Liu |
| author_sort | Xiao-Ling Wang |
| collection | DOAJ |
| description | A practical medium for reducing costs and enhancing both biomass and intracellular polysaccharide (IPS) production by a newly screened Agaricus blazei AB003 has been selected. The results show that IPS production was growth-associated, that a combination of corn flour extract and glucose was the best carbon source, and that a combination of wheat bran extract and yeast extract was the best nitrogen source for both biomass and IPS production. The effects of the four factors were further investigated using four-factor, three-level orthogonal test design. The best combination for the production of biomass and IPS was (in g/L): corn flour extract 15, glucose 6, yeast extract 3, and wheat bran extract 6. The maximum biomass and IPS in a 30-litre stirred tank bioreactor reached 11.30 and 0.72 g/L in the optimized medium, respectively. The use of two low-cost raw materials like corn flour and wheat bran extract as the major medium constituents could reduce the costs of biomass and IPS production. |
| first_indexed | 2024-03-09T09:24:34Z |
| format | Article |
| id | doaj.art-f3183d72623546e1830bff76f3cd5c1e |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T09:24:34Z |
| publishDate | 2009-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-f3183d72623546e1830bff76f3cd5c1e2023-12-02T06:45:01ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062009-01-01472210214Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003Xiao-Ling Wang0Gao-Qiang Liu1College of Life Sciences and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, CN-410004 Changsha, PR ChinaCollege of Life Sciences and Technology, Central South University of Forestry and Technology, 498 South Shaoshan Road, CN-410004 Changsha, PR ChinaA practical medium for reducing costs and enhancing both biomass and intracellular polysaccharide (IPS) production by a newly screened Agaricus blazei AB003 has been selected. The results show that IPS production was growth-associated, that a combination of corn flour extract and glucose was the best carbon source, and that a combination of wheat bran extract and yeast extract was the best nitrogen source for both biomass and IPS production. The effects of the four factors were further investigated using four-factor, three-level orthogonal test design. The best combination for the production of biomass and IPS was (in g/L): corn flour extract 15, glucose 6, yeast extract 3, and wheat bran extract 6. The maximum biomass and IPS in a 30-litre stirred tank bioreactor reached 11.30 and 0.72 g/L in the optimized medium, respectively. The use of two low-cost raw materials like corn flour and wheat bran extract as the major medium constituents could reduce the costs of biomass and IPS production.http://hrcak.srce.hr/file/59887Agaricus blazeiintracellular polysaccharidemycelial growthmedium selectionsubmerged fermentation |
| spellingShingle | Xiao-Ling Wang Gao-Qiang Liu Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003 Food Technology and Biotechnology Agaricus blazei intracellular polysaccharide mycelial growth medium selection submerged fermentation |
| title | Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003 |
| title_full | Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003 |
| title_fullStr | Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003 |
| title_full_unstemmed | Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003 |
| title_short | Selection of a Culture Medium for Reducing Costs and Enhancing Biomass and Intracellular Polysaccharide Production by Agaricus blazei AB2003 |
| title_sort | selection of a culture medium for reducing costs and enhancing biomass and intracellular polysaccharide production by agaricus blazei ab2003 |
| topic | Agaricus blazei intracellular polysaccharide mycelial growth medium selection submerged fermentation |
| url | http://hrcak.srce.hr/file/59887 |
| work_keys_str_mv | AT xiaolingwang selectionofaculturemediumforreducingcostsandenhancingbiomassandintracellularpolysaccharideproductionbyagaricusblazeiab2003 AT gaoqiangliu selectionofaculturemediumforreducingcostsandenhancingbiomassandintracellularpolysaccharideproductionbyagaricusblazeiab2003 |