Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses
Bunyamwera virus is the prototype of the Bunyamwera serogroup, which belongs to the order <i>Bunyavirales</i> of the <i>Orthobunyavirus</i> genus in the Peribunyaviridae family. Bunyamwera is a negative-sense RNA virus composed of three segments S, M, and L. Genetic recombina...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/1999-4915/15/2/550 |
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| author | Moufid Mhamadi Idrissa Dieng Anna S. Dolgova Cheikh Talibouya Touré Mignane Ndiaye Moussa Moïse Diagne Babacar Faye Anna S. Gladkikh Vladimir G. Dedkov Amadou Alpha Sall Ousmane Faye Oumar Faye |
| author_facet | Moufid Mhamadi Idrissa Dieng Anna S. Dolgova Cheikh Talibouya Touré Mignane Ndiaye Moussa Moïse Diagne Babacar Faye Anna S. Gladkikh Vladimir G. Dedkov Amadou Alpha Sall Ousmane Faye Oumar Faye |
| author_sort | Moufid Mhamadi |
| collection | DOAJ |
| description | Bunyamwera virus is the prototype of the Bunyamwera serogroup, which belongs to the order <i>Bunyavirales</i> of the <i>Orthobunyavirus</i> genus in the Peribunyaviridae family. Bunyamwera is a negative-sense RNA virus composed of three segments S, M, and L. Genetic recombination is possible between members of this order as it is already documented. Additionally, it can lead to pathogenic or host range improvement, if it occurs with viruses of public health and agricultural importance such as Rift Valley fever virus and Crimea–Congo hemorrhagic fever virus. Here, we characterize five African <i>Orthobunyavirus</i> viruses from different geographical regions. Our results suggest that the five newly characterized strains are identified as Bunyamwera virus strains. Furthermore, two of the five strains sequenced in this study are recombinant strains, as fragments of their segments are carried by Ngari and Bunyamwera strains. Further investigations are needed to understand the functional impact of these recombinations. |
| first_indexed | 2024-03-11T08:01:15Z |
| format | Article |
| id | doaj.art-f318a5b98e7d47b6951c70d42c943aa0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T08:01:15Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-f318a5b98e7d47b6951c70d42c943aa02023-11-16T23:50:32ZengMDPI AGViruses1999-49152023-02-0115255010.3390/v15020550Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari VirusesMoufid Mhamadi0Idrissa Dieng1Anna S. Dolgova2Cheikh Talibouya Touré3Mignane Ndiaye4Moussa Moïse Diagne5Babacar Faye6Anna S. Gladkikh7Vladimir G. Dedkov8Amadou Alpha Sall9Ousmane Faye10Oumar Faye11Virology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalSaint Petersburg Pasteur Institute, 197101 Saint Petersburg, RussiaVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalParasitology Department, Université Cheikh Anta Diop de Dakar, Dakar 10700, SenegalSaint Petersburg Pasteur Institute, 197101 Saint Petersburg, RussiaSaint Petersburg Pasteur Institute, 197101 Saint Petersburg, RussiaVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalVirology Department, Institut Pasteur de Dakar, Dakar 12900, SenegalBunyamwera virus is the prototype of the Bunyamwera serogroup, which belongs to the order <i>Bunyavirales</i> of the <i>Orthobunyavirus</i> genus in the Peribunyaviridae family. Bunyamwera is a negative-sense RNA virus composed of three segments S, M, and L. Genetic recombination is possible between members of this order as it is already documented. Additionally, it can lead to pathogenic or host range improvement, if it occurs with viruses of public health and agricultural importance such as Rift Valley fever virus and Crimea–Congo hemorrhagic fever virus. Here, we characterize five African <i>Orthobunyavirus</i> viruses from different geographical regions. Our results suggest that the five newly characterized strains are identified as Bunyamwera virus strains. Furthermore, two of the five strains sequenced in this study are recombinant strains, as fragments of their segments are carried by Ngari and Bunyamwera strains. Further investigations are needed to understand the functional impact of these recombinations.https://www.mdpi.com/1999-4915/15/2/550Bunyamweraserogroupsegmented RNAgenetic recombination |
| spellingShingle | Moufid Mhamadi Idrissa Dieng Anna S. Dolgova Cheikh Talibouya Touré Mignane Ndiaye Moussa Moïse Diagne Babacar Faye Anna S. Gladkikh Vladimir G. Dedkov Amadou Alpha Sall Ousmane Faye Oumar Faye Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses Viruses Bunyamwera serogroup segmented RNA genetic recombination |
| title | Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses |
| title_full | Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses |
| title_fullStr | Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses |
| title_full_unstemmed | Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses |
| title_short | Whole Genome Sequencing Analysis of African Orthobunyavirus Isolates Reveals Naturally Interspecies Segments Recombinations between Bunyamwera and Ngari Viruses |
| title_sort | whole genome sequencing analysis of african orthobunyavirus isolates reveals naturally interspecies segments recombinations between bunyamwera and ngari viruses |
| topic | Bunyamwera serogroup segmented RNA genetic recombination |
| url | https://www.mdpi.com/1999-4915/15/2/550 |
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