Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla
ObjectiveAssessment of the cytotoxicity of novel calcium silicate-based cement is imperative in endodontics. Thisexperimental study aimed to assess the cytotoxicity and odontogenic/osteogenic differentiation potential of a newcalcium silicate/pectin cement called Nano-dentine against stem cells from...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2022-11-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_253341_158c7ebd814cabc4ff59623a62b95c79.pdf |
| _version_ | 1798015073457799168 |
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| author | Eshagh Ali Saberi Narges Farhad Mollashahi Fatemeh Ejeian Marzieh Nematollahi Omolbanin Shahraki Arezou Pirhaji Mohammad Hossein Nasr Esfahani |
| author_facet | Eshagh Ali Saberi Narges Farhad Mollashahi Fatemeh Ejeian Marzieh Nematollahi Omolbanin Shahraki Arezou Pirhaji Mohammad Hossein Nasr Esfahani |
| author_sort | Eshagh Ali Saberi |
| collection | DOAJ |
| description | ObjectiveAssessment of the cytotoxicity of novel calcium silicate-based cement is imperative in endodontics. Thisexperimental study aimed to assess the cytotoxicity and odontogenic/osteogenic differentiation potential of a newcalcium silicate/pectin cement called Nano-dentine against stem cells from the apical papilla (SCAPs).Materials and MethodsIn this experimental study, the cement powder was synthesized by the sol-gel technique.Zirconium oxide was added as opacifier and Pectin, a plant-based polymer, and calcium chloride as the liquid toprepare the nano-based dental cement. Thirty-six root canal dentin blocks of human extracted single-canal premolarswith 2 mm height, flared with #1, 2 and 3 Gates-Glidden drills were used to prepare the cement specimens. Thecement, namely mineral trioxide aggregate (MTA), Biodentine, and the Nano-dentine were mixed according to themanufacturers’ instructions and applied to the roots of canal dentin blocks. The cytotoxicity and odontogenic/osteogenicpotential of the cement were evaluated by using SCAPs.ResultsSCAPs were characterized by the expression of routine mesenchymal cell markers and differentiationpotential to adipocytes, osteoblasts, and chondrocytes. Cement displayed no significant differences in cytotoxicity orcalcified nodules formation. Gene expression analysis showed that all three types of cement induced significant downregulationof COLA1; however, the new cement induced significant up-regulation of RUNX2 and SPP1 compared tothe control group and MTA. The new cement also induced significant up-regulation of TGFB1 and inducible nitric oxidesynthase (iNOS) compared with Biodentine and MTA.ConclusionThe new Nano-dentin cement has higher odontogenic/osteogenic potential compared to Biodentine andMTA for differentiation of SCAPs to adipocytes, osteoblasts, and chondrocytes. |
| first_indexed | 2024-04-11T15:27:26Z |
| format | Article |
| id | doaj.art-f320fafc0ff84c6ea6740ff7208a2639 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-11T15:27:26Z |
| publishDate | 2022-11-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-f320fafc0ff84c6ea6740ff7208a26392022-12-22T04:16:13ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142022-11-01241163764610.22074/cellj.2022.8126253341Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical PapillaEshagh Ali Saberi0Narges Farhad Mollashahi1Fatemeh Ejeian2Marzieh Nematollahi3Omolbanin Shahraki4Arezou Pirhaji5Mohammad Hossein Nasr Esfahani6Department of Endodontics, Faculty of Dentistry, Oral and Dental Diseases Research Center, Zahedan University of Medical Sciences, Zahedan, IranDepartment of Endodontics, Faculty of Dentistry, Oral and Dental Diseases Research Center, Zahedan University of Medical Sciences, Zahedan, IranDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranDepartment of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranPharmacology Research Center, Zahedan University of Medical Sciences, Zahedan, IranDepartment of Endodontics, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.Department of Animal Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, IranObjectiveAssessment of the cytotoxicity of novel calcium silicate-based cement is imperative in endodontics. Thisexperimental study aimed to assess the cytotoxicity and odontogenic/osteogenic differentiation potential of a newcalcium silicate/pectin cement called Nano-dentine against stem cells from the apical papilla (SCAPs).Materials and MethodsIn this experimental study, the cement powder was synthesized by the sol-gel technique.Zirconium oxide was added as opacifier and Pectin, a plant-based polymer, and calcium chloride as the liquid toprepare the nano-based dental cement. Thirty-six root canal dentin blocks of human extracted single-canal premolarswith 2 mm height, flared with #1, 2 and 3 Gates-Glidden drills were used to prepare the cement specimens. Thecement, namely mineral trioxide aggregate (MTA), Biodentine, and the Nano-dentine were mixed according to themanufacturers’ instructions and applied to the roots of canal dentin blocks. The cytotoxicity and odontogenic/osteogenicpotential of the cement were evaluated by using SCAPs.ResultsSCAPs were characterized by the expression of routine mesenchymal cell markers and differentiationpotential to adipocytes, osteoblasts, and chondrocytes. Cement displayed no significant differences in cytotoxicity orcalcified nodules formation. Gene expression analysis showed that all three types of cement induced significant downregulationof COLA1; however, the new cement induced significant up-regulation of RUNX2 and SPP1 compared tothe control group and MTA. The new cement also induced significant up-regulation of TGFB1 and inducible nitric oxidesynthase (iNOS) compared with Biodentine and MTA.ConclusionThe new Nano-dentin cement has higher odontogenic/osteogenic potential compared to Biodentine andMTA for differentiation of SCAPs to adipocytes, osteoblasts, and chondrocytes.https://www.celljournal.org/article_253341_158c7ebd814cabc4ff59623a62b95c79.pdfbiodentinecalcium silicatemineral trioxide aggregatestem cells |
| spellingShingle | Eshagh Ali Saberi Narges Farhad Mollashahi Fatemeh Ejeian Marzieh Nematollahi Omolbanin Shahraki Arezou Pirhaji Mohammad Hossein Nasr Esfahani Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla Cell Journal biodentine calcium silicate mineral trioxide aggregate stem cells |
| title | Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla |
| title_full | Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla |
| title_fullStr | Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla |
| title_full_unstemmed | Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla |
| title_short | Assessment of Cytotoxicity and Odontogenic/Osteogenic Differentiation Potential of Nano-Dentine Cement Against Stem Cells from Apical Papilla |
| title_sort | assessment of cytotoxicity and odontogenic osteogenic differentiation potential of nano dentine cement against stem cells from apical papilla |
| topic | biodentine calcium silicate mineral trioxide aggregate stem cells |
| url | https://www.celljournal.org/article_253341_158c7ebd814cabc4ff59623a62b95c79.pdf |
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