Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera
Abstract Background Stem cell-based therapies are an attractive option to promote regeneration and repair defective tissues and organs. Thanks to their multipotency, high proliferation rate and the lack of major ethical limitations, “olfactory ecto-mesenchymal stem cells” (OE-MSCs) have been describ...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-01-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-018-1342-2 |
| _version_ | 1830515753138257920 |
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| author | Antoine D. Veron Cécile Bienboire-Frosini François Feron Elisa Codecasa Arnaud Deveze Dany Royer Paul Watelet Pietro Asproni Kevin Sadelli Camille Chabaud Jean-claude Stamegna Joël Fagot Michel Khrestchatisky Alessandro Cozzi François S. Roman Patrick Pageat Manuel Mengoli Stéphane D. Girard |
| author_facet | Antoine D. Veron Cécile Bienboire-Frosini François Feron Elisa Codecasa Arnaud Deveze Dany Royer Paul Watelet Pietro Asproni Kevin Sadelli Camille Chabaud Jean-claude Stamegna Joël Fagot Michel Khrestchatisky Alessandro Cozzi François S. Roman Patrick Pageat Manuel Mengoli Stéphane D. Girard |
| author_sort | Antoine D. Veron |
| collection | DOAJ |
| description | Abstract Background Stem cell-based therapies are an attractive option to promote regeneration and repair defective tissues and organs. Thanks to their multipotency, high proliferation rate and the lack of major ethical limitations, “olfactory ecto-mesenchymal stem cells” (OE-MSCs) have been described as a promising candidate to treat a variety of damaged tissues. Easily accessible in the nasal cavity of most mammals, these cells are highly suitable for autologous cell-based therapies and do not face issues associated with other stem cells. However, their clinical use in humans and animals is limited due to a lack of preclinical studies on autologous transplantation and because no well-established methods currently exist to cultivate these cells. Here we evaluated the feasibility of collecting, purifying and amplifying OE-MSCs from different mammalian genera with the goal of promoting their interest in veterinary regenerative medicine. Biopsies of olfactory mucosa from eight mammalian genera (mouse, rat, rabbit, sheep, dog, horse, gray mouse lemur and macaque) were collected, using techniques derived from those previously used in humans and rats. The possibility of amplifying these cells and their stemness features and differentiation capability were then evaluated. Results Biopsies were successfully performed on olfactory mucosa without requiring the sacrifice of the donor animal, except mice. Cell populations were rapidly generated from olfactory mucosa explants. These cells displayed similar key features of their human counterparts: a fibroblastic morphology, a robust expression of nestin, an ability to form spheres and similar expression of surface markers (CD44, CD73). Moreover, most of them also exhibited high proliferation rates and clonogenicity with genus-specific properties. Finally, OE-MSCs also showed the ability to differentiate into mesodermal lineages. Conclusions This article describes for the first time how millions of OE-MSCs can be quickly and easily obtained from different mammalian genera through protocols that are well-suited for autologous transplantations. Moreover, their multipotency makes them relevant to evaluate therapeutic application in a wide variety of tissue injury models. This study paves the way for the development of new fundamental and clinical studies based on OE-MSCs transplantation and suggests their interest in veterinary medicine. |
| first_indexed | 2024-12-22T03:28:44Z |
| format | Article |
| id | doaj.art-f321577a2c7f460387308772bb17da00 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-22T03:28:44Z |
| publishDate | 2018-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-f321577a2c7f460387308772bb17da002022-12-21T18:40:33ZengBMCBMC Veterinary Research1746-61482018-01-0114111110.1186/s12917-018-1342-2Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian generaAntoine D. Veron0Cécile Bienboire-Frosini1François Feron2Elisa Codecasa3Arnaud Deveze4Dany Royer5Paul Watelet6Pietro Asproni7Kevin Sadelli8Camille Chabaud9Jean-claude Stamegna10Joël Fagot11Michel Khrestchatisky12Alessandro Cozzi13François S. Roman14Patrick Pageat15Manuel Mengoli16Stéphane D. Girard17IRSEA, Research Institute in Semiochemistry and Applied EthologyIRSEA, Research Institute in Semiochemistry and Applied EthologyAix Marseille Univ, CNRS, NICNIRSEA, Research Institute in Semiochemistry and Applied EthologyDépartement ORL, Hôpital Universitaire Nord, AP-HMCentre Hospitalier Vétérinaire PommerySociété Hippique Le frigouyéIRSEA, Research Institute in Semiochemistry and Applied EthologyAix Marseille Univ, CNRS, NICNIRSEA, Research Institute in Semiochemistry and Applied EthologyAix Marseille Univ, CNRS, NICNAix-Marseille Univ, CNRS, LPCAix Marseille Univ, CNRS, NICNIRSEA, Research Institute in Semiochemistry and Applied EthologyAix Marseille Univ, CNRS, NICNIRSEA, Research Institute in Semiochemistry and Applied EthologyIRSEA, Research Institute in Semiochemistry and Applied EthologyAix Marseille Univ, CNRS, NICNAbstract Background Stem cell-based therapies are an attractive option to promote regeneration and repair defective tissues and organs. Thanks to their multipotency, high proliferation rate and the lack of major ethical limitations, “olfactory ecto-mesenchymal stem cells” (OE-MSCs) have been described as a promising candidate to treat a variety of damaged tissues. Easily accessible in the nasal cavity of most mammals, these cells are highly suitable for autologous cell-based therapies and do not face issues associated with other stem cells. However, their clinical use in humans and animals is limited due to a lack of preclinical studies on autologous transplantation and because no well-established methods currently exist to cultivate these cells. Here we evaluated the feasibility of collecting, purifying and amplifying OE-MSCs from different mammalian genera with the goal of promoting their interest in veterinary regenerative medicine. Biopsies of olfactory mucosa from eight mammalian genera (mouse, rat, rabbit, sheep, dog, horse, gray mouse lemur and macaque) were collected, using techniques derived from those previously used in humans and rats. The possibility of amplifying these cells and their stemness features and differentiation capability were then evaluated. Results Biopsies were successfully performed on olfactory mucosa without requiring the sacrifice of the donor animal, except mice. Cell populations were rapidly generated from olfactory mucosa explants. These cells displayed similar key features of their human counterparts: a fibroblastic morphology, a robust expression of nestin, an ability to form spheres and similar expression of surface markers (CD44, CD73). Moreover, most of them also exhibited high proliferation rates and clonogenicity with genus-specific properties. Finally, OE-MSCs also showed the ability to differentiate into mesodermal lineages. Conclusions This article describes for the first time how millions of OE-MSCs can be quickly and easily obtained from different mammalian genera through protocols that are well-suited for autologous transplantations. Moreover, their multipotency makes them relevant to evaluate therapeutic application in a wide variety of tissue injury models. This study paves the way for the development of new fundamental and clinical studies based on OE-MSCs transplantation and suggests their interest in veterinary medicine.http://link.springer.com/article/10.1186/s12917-018-1342-2Adult craniofacial stem cellsDogEcto-mesenchymal stem cellsHorseNon-human primateRabbit |
| spellingShingle | Antoine D. Veron Cécile Bienboire-Frosini François Feron Elisa Codecasa Arnaud Deveze Dany Royer Paul Watelet Pietro Asproni Kevin Sadelli Camille Chabaud Jean-claude Stamegna Joël Fagot Michel Khrestchatisky Alessandro Cozzi François S. Roman Patrick Pageat Manuel Mengoli Stéphane D. Girard Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera BMC Veterinary Research Adult craniofacial stem cells Dog Ecto-mesenchymal stem cells Horse Non-human primate Rabbit |
| title | Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera |
| title_full | Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera |
| title_fullStr | Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera |
| title_full_unstemmed | Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera |
| title_short | Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera |
| title_sort | isolation and characterization of olfactory ecto mesenchymal stem cells from eight mammalian genera |
| topic | Adult craniofacial stem cells Dog Ecto-mesenchymal stem cells Horse Non-human primate Rabbit |
| url | http://link.springer.com/article/10.1186/s12917-018-1342-2 |
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