Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia
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| Main Authors: | , |
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| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2012-06-01
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| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31703 |
| _version_ | 1818060480389840896 |
|---|---|
| author | Seir Antonio Salazar Mercado Giovanni Orlando Cancino |
| author_facet | Seir Antonio Salazar Mercado Giovanni Orlando Cancino |
| author_sort | Seir Antonio Salazar Mercado |
| collection | DOAJ |
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</style> <![endif]--> <p class="MsoNormal" style="text-align: justify; mso-outline-level: 1;"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Título en ingles: Evaluation of the </span></strong></span><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; color: black; mso-ansi-language: EN-US;" lang="EN-US">effects of two organics supplements on <em style="mso-bidi-font-style: normal;">in vitro</em> germination of native orchids in the province of Pamplona, Colombia</span></strong></p> <p class="Default" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-fareast-language: ES;" lang="ES">Resumen: </span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES">La continua pérdida de hábitat de las orquídeas nativas en Colombia, y las limitaciones de la germinación en estado silvestre, ha dado lugar a un mayor énfasis en la conservación de las orquídeas. Por consiguiente el cultivo <em style="mso-bidi-font-style: normal;">in vitro </em>es una herramienta alternativa para la conservación de especies en peligro de extinción. En esta investigación se evaluó la germinación asimbiótica y la formación de plántulas de semillas de orquídeas, de las especies <em style="mso-bidi-font-style: normal;">Prosthechea vespa </em></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial;" lang="ES">Vell.</span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES"> </span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES">y <em style="mso-bidi-font-style: normal;">Sobralia klotzscheana</em> </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial;" lang="ES">Rchb. f. </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES">en el medio de cultivo Murashige y Skoog (MS) con dos suplementos orgánicos (jugo de piña y agua de coco). Se colectaron cápsulas maduras de las especies <em style="mso-bidi-font-style: normal;">P. vespa </em>y <em style="mso-bidi-font-style: normal;">S. klotzscheana</em>, en la región nororiental de Colombia (Pamplona, Norte de Santander) y se determinó la viabilidad de las semillas con la prueba de Tetrazolio. Las semillas se desinfectaron y se sembraron con el método de la jeringuilla. La viabilidad de las semillas fue del 87,2% en <em style="mso-bidi-font-style: normal;">P. vespa</em> y 80,6% en <em style="mso-bidi-font-style: normal;">S. klotzscheana</em>. El porcentaje de viabilidad corregido con respecto a la germinación fue mayor, entre 2,8% (<em style="mso-bidi-font-style: normal;">P. vespa</em>) y 0,5% (<em style="mso-bidi-font-style: normal;">S. klotzscheana</em>). Este estudio demostró que el medio MS suplementado con jugo de piña tiene una mayor respuesta a la geminación asimbiótica y formación de plántulas en las orquídeas <em style="mso-bidi-font-style: normal;">P. vespa </em>(22%) y <em style="mso-bidi-font-style: normal;">S. klotzscheana </em>(43%) con diferencias estadísticamente significativas </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial;" lang="ES">(P≤0,05: Tukey HSD<em style="mso-bidi-font-style: normal;">).</em></span></p> <p class="MsoNormal" style="text-align: justify; mso-layout-grid-align: none; text-autospace: none;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; color: black;">Palabras clave: </span></strong><span style="font-size: 10.0pt; color: black;">conservación, germinación asimbiótica, orquídeas, viabilidad, tetrazolio.</span></p> <p class="Default" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Abstract : </span></strong><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">The continued</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> <span class="hps">habitat destruction of</span> <span class="hps">native orchids</span> <span class="hps">in Colombia</span> and its germination limitations <span class="hps">in the wild</span> <span class="hps">has led</span> <span class="hps">to greater</span> <span class="hps">emphasis of the species conservation, therefore to preserve endangered orchids <em style="mso-bidi-font-style: normal;">in vit</em>ro culture is an alternative tool. This research evaluated orchid seeds</span> <span class="hps">asymbiotic</span> <span class="hps">germination</span> <span class="hps">and</span> <span class="hps">seedling</span> <span class="hps">formation of </span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">Prosthechea vespa </span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US;" lang="EN-US">Vell.</span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> </span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">and <em style="mso-bidi-font-style: normal;">Sobralia klotzscheana</em> </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Rchb. f. <span class="hps">species</span> <span class="hps">in</span> <span class="hps">Murashige</span> <span class="hps">and</span> <span class="hps">Skoog</span> <span class="hps">(MS</span>) <span class="hps">culture medium</span> with two <span class="hps">organic supplements </span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">(pineapple juice and coconut water)</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">.</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> Mature capsules from <em style="mso-bidi-font-style: normal;">P. </em></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> and <em style="mso-bidi-font-style: normal;">S. klotzscheana</em> species were collected in the Province of Pamplona, northeastern region of Colombia and their viability with tetrazolium staining was determined. The seeds were disinfected and sown by the syringe method.</span><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> The seeds viability were</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> <span class="hps">87,2% in </span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">P. vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> </span><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">and 80,6</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">% in </span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">S. klotzscheana</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">.</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> <span class="hps">The</span> <span class="hps">corrected</span> <span class="hps">percentage of viability</span> <span class="hps">compared</span> <span class="hps">to the germination</span> <span class="hps">was higher</span>, between 2,8<span class="atn">% (</span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">P. </span></em><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">) and 0,5<span class="atn">% (</span><span class="hps"><em style="mso-bidi-font-style: normal;">S.</em></span><em style="mso-bidi-font-style: normal;"> <span class="hps">klotzscheana</span>).</em> <span class="hps">This study showed that</span> <span class="hps">MS medium</span> <span class="hps">supplemented</span> <span class="hps">with pineapple juice</span> <span class="hps">has a</span> <span class="hps">greater response to</span> <span class="hps">asymbiotic</span> <span class="hps">germination</span> <span class="hps">and</span> <span class="hps">seedling</span> <span class="hps">formation</span> <span class="hps">in </span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">P. </span></em><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> </span><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">(22</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">%) and <span class="hps"><em style="mso-bidi-font-style: normal;">S.</em></span><em style="mso-bidi-font-style: normal;"> <span class="hps">klotzscheana</span></em> <span class="hps">(43</span>%) <span class="hps">with statistically significant differences</span> (P≤0,05: Tukey HSD<em style="mso-bidi-font-style: normal;">).</em></span></p> <p class="Default" style="text-align: justify;"><span class="hps"></span><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; color: black; mso-ansi-language: EN-US; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US">Key words:</span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; color: black; mso-ansi-language: EN-US; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US"> conservation,<span class="hps"> asymbiotic</span><span class="shorttext"> </span><span class="hps">germination,</span> orchids, viability, tetrazolium</span></p> |
| first_indexed | 2024-12-10T13:33:05Z |
| format | Article |
| id | doaj.art-f325fb3cb433415484905e3c99152a0e |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-10T13:33:05Z |
| publishDate | 2012-06-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-f325fb3cb433415484905e3c99152a0e2022-12-22T01:46:54ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582012-06-01141535927967Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, ColombiaSeir Antonio Salazar Mercado0Giovanni Orlando Cancino1InvestigadorInvestigador<!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <w:TrackMoves /> <w:TrackFormatting /> <w:HyphenationZone>21</w:HyphenationZone> <w:PunctuationKerning /> <w:ValidateAgainstSchemas /> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:DoNotPromoteQF /> <w:LidThemeOther>ES-CO</w:LidThemeOther> <w:LidThemeAsian>X-NONE</w:LidThemeAsian> <w:LidThemeComplexScript>X-NONE</w:LidThemeComplexScript> <w:Compatibility> <w:BreakWrappedTables /> 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mso-hansi-theme-font:minor-latin;} </style> <![endif]--> <p class="MsoNormal" style="text-align: justify; mso-outline-level: 1;"><span class="hps"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; mso-ansi-language: EN-US;" lang="EN-US">Título en ingles: Evaluation of the </span></strong></span><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; color: black; mso-ansi-language: EN-US;" lang="EN-US">effects of two organics supplements on <em style="mso-bidi-font-style: normal;">in vitro</em> germination of native orchids in the province of Pamplona, Colombia</span></strong></p> <p class="Default" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-fareast-language: ES;" lang="ES">Resumen: </span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES">La continua pérdida de hábitat de las orquídeas nativas en Colombia, y las limitaciones de la germinación en estado silvestre, ha dado lugar a un mayor énfasis en la conservación de las orquídeas. Por consiguiente el cultivo <em style="mso-bidi-font-style: normal;">in vitro </em>es una herramienta alternativa para la conservación de especies en peligro de extinción. En esta investigación se evaluó la germinación asimbiótica y la formación de plántulas de semillas de orquídeas, de las especies <em style="mso-bidi-font-style: normal;">Prosthechea vespa </em></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial;" lang="ES">Vell.</span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES"> </span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES">y <em style="mso-bidi-font-style: normal;">Sobralia klotzscheana</em> </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial;" lang="ES">Rchb. f. </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-fareast-language: ES-CO;" lang="ES">en el medio de cultivo Murashige y Skoog (MS) con dos suplementos orgánicos (jugo de piña y agua de coco). Se colectaron cápsulas maduras de las especies <em style="mso-bidi-font-style: normal;">P. vespa </em>y <em style="mso-bidi-font-style: normal;">S. klotzscheana</em>, en la región nororiental de Colombia (Pamplona, Norte de Santander) y se determinó la viabilidad de las semillas con la prueba de Tetrazolio. Las semillas se desinfectaron y se sembraron con el método de la jeringuilla. La viabilidad de las semillas fue del 87,2% en <em style="mso-bidi-font-style: normal;">P. vespa</em> y 80,6% en <em style="mso-bidi-font-style: normal;">S. klotzscheana</em>. El porcentaje de viabilidad corregido con respecto a la germinación fue mayor, entre 2,8% (<em style="mso-bidi-font-style: normal;">P. vespa</em>) y 0,5% (<em style="mso-bidi-font-style: normal;">S. klotzscheana</em>). Este estudio demostró que el medio MS suplementado con jugo de piña tiene una mayor respuesta a la geminación asimbiótica y formación de plántulas en las orquídeas <em style="mso-bidi-font-style: normal;">P. vespa </em>(22%) y <em style="mso-bidi-font-style: normal;">S. klotzscheana </em>(43%) con diferencias estadísticamente significativas </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial;" lang="ES">(P≤0,05: Tukey HSD<em style="mso-bidi-font-style: normal;">).</em></span></p> <p class="MsoNormal" style="text-align: justify; mso-layout-grid-align: none; text-autospace: none;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; color: black;">Palabras clave: </span></strong><span style="font-size: 10.0pt; color: black;">conservación, germinación asimbiótica, orquídeas, viabilidad, tetrazolio.</span></p> <p class="Default" style="text-align: justify;"><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Abstract : </span></strong><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">The continued</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> <span class="hps">habitat destruction of</span> <span class="hps">native orchids</span> <span class="hps">in Colombia</span> and its germination limitations <span class="hps">in the wild</span> <span class="hps">has led</span> <span class="hps">to greater</span> <span class="hps">emphasis of the species conservation, therefore to preserve endangered orchids <em style="mso-bidi-font-style: normal;">in vit</em>ro culture is an alternative tool. This research evaluated orchid seeds</span> <span class="hps">asymbiotic</span> <span class="hps">germination</span> <span class="hps">and</span> <span class="hps">seedling</span> <span class="hps">formation of </span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">Prosthechea vespa </span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-ansi-language: EN-US;" lang="EN-US">Vell.</span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> </span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">and <em style="mso-bidi-font-style: normal;">Sobralia klotzscheana</em> </span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">Rchb. f. <span class="hps">species</span> <span class="hps">in</span> <span class="hps">Murashige</span> <span class="hps">and</span> <span class="hps">Skoog</span> <span class="hps">(MS</span>) <span class="hps">culture medium</span> with two <span class="hps">organic supplements </span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">(pineapple juice and coconut water)</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">.</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> Mature capsules from <em style="mso-bidi-font-style: normal;">P. </em></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> and <em style="mso-bidi-font-style: normal;">S. klotzscheana</em> species were collected in the Province of Pamplona, northeastern region of Colombia and their viability with tetrazolium staining was determined. The seeds were disinfected and sown by the syringe method.</span><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> The seeds viability were</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> <span class="hps">87,2% in </span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">P. vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> </span><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">and 80,6</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">% in </span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">S. klotzscheana</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">.</span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US"> <span class="hps">The</span> <span class="hps">corrected</span> <span class="hps">percentage of viability</span> <span class="hps">compared</span> <span class="hps">to the germination</span> <span class="hps">was higher</span>, between 2,8<span class="atn">% (</span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">P. </span></em><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">) and 0,5<span class="atn">% (</span><span class="hps"><em style="mso-bidi-font-style: normal;">S.</em></span><em style="mso-bidi-font-style: normal;"> <span class="hps">klotzscheana</span>).</em> <span class="hps">This study showed that</span> <span class="hps">MS medium</span> <span class="hps">supplemented</span> <span class="hps">with pineapple juice</span> <span class="hps">has a</span> <span class="hps">greater response to</span> <span class="hps">asymbiotic</span> <span class="hps">germination</span> <span class="hps">and</span> <span class="hps">seedling</span> <span class="hps">formation</span> <span class="hps">in </span></span><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">P. </span></em><em style="mso-bidi-font-style: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US">vespa</span></em><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US; mso-fareast-language: ES-CO;" lang="EN-US"> </span><span class="hps"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">(22</span></span><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-bidi-font-family: Arial; mso-ansi-language: EN-US;" lang="EN-US">%) and <span class="hps"><em style="mso-bidi-font-style: normal;">S.</em></span><em style="mso-bidi-font-style: normal;"> <span class="hps">klotzscheana</span></em> <span class="hps">(43</span>%) <span class="hps">with statistically significant differences</span> (P≤0,05: Tukey HSD<em style="mso-bidi-font-style: normal;">).</em></span></p> <p class="Default" style="text-align: justify;"><span class="hps"></span><strong style="mso-bidi-font-weight: normal;"><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; color: black; mso-ansi-language: EN-US; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US">Key words:</span></strong><span style="font-size: 10.0pt; font-family: ";Times New Roman";,";serif";; mso-fareast-font-family: ";Times New Roman";; color: black; mso-ansi-language: EN-US; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US"> conservation,<span class="hps"> asymbiotic</span><span class="shorttext"> </span><span class="hps">germination,</span> orchids, viability, tetrazolium</span></p>http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31703conservacióngerminación asimbióticaorquídeasviabilidadtetrazolioconservationasymbiotic germinationorchidsviabilitytetrazolium |
| spellingShingle | Seir Antonio Salazar Mercado Giovanni Orlando Cancino Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia Revista Colombiana de Biotecnología conservación germinación asimbiótica orquídeas viabilidad tetrazolio conservation asymbiotic germination orchids viability tetrazolium |
| title | Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia |
| title_full | Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia |
| title_fullStr | Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia |
| title_full_unstemmed | Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia |
| title_short | Evaluación del efecto de dos suplementos orgánicos en la germinación in vitro de orquídeas nativas de la provincia de Pamplona, Colombia |
| title_sort | evaluacion del efecto de dos suplementos organicos en la germinacion in vitro de orquideas nativas de la provincia de pamplona colombia |
| topic | conservación germinación asimbiótica orquídeas viabilidad tetrazolio conservation asymbiotic germination orchids viability tetrazolium |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/31703 |
| work_keys_str_mv | AT seirantoniosalazarmercado evaluaciondelefectodedossuplementosorganicosenlagerminacioninvitrodeorquideasnativasdelaprovinciadepamplonacolombia AT giovanniorlandocancino evaluaciondelefectodedossuplementosorganicosenlagerminacioninvitrodeorquideasnativasdelaprovinciadepamplonacolombia |