Assays for phenotypic and functional characterization of cryopreserved platelets
Platelet concentrates for transfusion have a limited shelf-life, and cryopreservation offers a means of extending platelet shelf-life for at least 2 years. Cryopreservation, however, has some disadvantages, as platelets can be damaged during the freezing and thawing process. Consequently the phenoty...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2018.1514108 |
| _version_ | 1797684238394327040 |
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| author | Denese C Marks Lacey Johnson |
| author_facet | Denese C Marks Lacey Johnson |
| author_sort | Denese C Marks |
| collection | DOAJ |
| description | Platelet concentrates for transfusion have a limited shelf-life, and cryopreservation offers a means of extending platelet shelf-life for at least 2 years. Cryopreservation, however, has some disadvantages, as platelets can be damaged during the freezing and thawing process. Consequently the phenotype of cryopreserved platelets is very different to that of freshly collected, liquid stored platelets. To obtain a reliable overview of cryopreserved platelet quality and function, specific testing methodologies should be considered. Whilst light transmission aggregometry (LTA) is the gold standard for measuring in vitro platelet reactivity in liquid stored platelets, it is not suited for cryopreserved platelets, as they retain little response to typical platelet agonists, even at high doses. Instead, phenotypic characterization by flow cytometry in combination with global assays of coagulation provides a clearer delineation of the phenotype and function of cryopreserved platelets. With increasing international uptake of platelet cryopreservation, it is important to recognize the need for appropriate measures of platelet quality and function. |
| first_indexed | 2024-03-12T00:27:43Z |
| format | Article |
| id | doaj.art-f329364b5d4d4363b8fa1f5d4421b715 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:27:43Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-f329364b5d4d4363b8fa1f5d4421b7152023-09-15T10:32:00ZengTaylor & Francis GroupPlatelets0953-71041369-16352019-01-01301485510.1080/09537104.2018.15141081514108Assays for phenotypic and functional characterization of cryopreserved plateletsDenese C Marks0Lacey Johnson1Australian Red Cross Blood ServiceAustralian Red Cross Blood ServicePlatelet concentrates for transfusion have a limited shelf-life, and cryopreservation offers a means of extending platelet shelf-life for at least 2 years. Cryopreservation, however, has some disadvantages, as platelets can be damaged during the freezing and thawing process. Consequently the phenotype of cryopreserved platelets is very different to that of freshly collected, liquid stored platelets. To obtain a reliable overview of cryopreserved platelet quality and function, specific testing methodologies should be considered. Whilst light transmission aggregometry (LTA) is the gold standard for measuring in vitro platelet reactivity in liquid stored platelets, it is not suited for cryopreserved platelets, as they retain little response to typical platelet agonists, even at high doses. Instead, phenotypic characterization by flow cytometry in combination with global assays of coagulation provides a clearer delineation of the phenotype and function of cryopreserved platelets. With increasing international uptake of platelet cryopreservation, it is important to recognize the need for appropriate measures of platelet quality and function.http://dx.doi.org/10.1080/09537104.2018.1514108aggregationannexin-vcryopreservationdmsoplateletsthrombin generation |
| spellingShingle | Denese C Marks Lacey Johnson Assays for phenotypic and functional characterization of cryopreserved platelets Platelets aggregation annexin-v cryopreservation dmso platelets thrombin generation |
| title | Assays for phenotypic and functional characterization of cryopreserved platelets |
| title_full | Assays for phenotypic and functional characterization of cryopreserved platelets |
| title_fullStr | Assays for phenotypic and functional characterization of cryopreserved platelets |
| title_full_unstemmed | Assays for phenotypic and functional characterization of cryopreserved platelets |
| title_short | Assays for phenotypic and functional characterization of cryopreserved platelets |
| title_sort | assays for phenotypic and functional characterization of cryopreserved platelets |
| topic | aggregation annexin-v cryopreservation dmso platelets thrombin generation |
| url | http://dx.doi.org/10.1080/09537104.2018.1514108 |
| work_keys_str_mv | AT denesecmarks assaysforphenotypicandfunctionalcharacterizationofcryopreservedplatelets AT laceyjohnson assaysforphenotypicandfunctionalcharacterizationofcryopreservedplatelets |