First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia
Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque was successfully isolated from soil of potato fields in Ngablak, Magelang. This research aimed to obtain the knowledge of P. vexans potency as a pathogen in potatoes, and also morphologically and molecularly identify ...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada
2021-12-01
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| Series: | Jurnal Perlindungan Tanaman Indonesia |
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| Online Access: | https://jurnal.ugm.ac.id/jpti/article/view/67556 |
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| author | Islaminati Anna Santika Ani Widiastuti Arif Wibowo |
| author_facet | Islaminati Anna Santika Ani Widiastuti Arif Wibowo |
| author_sort | Islaminati Anna Santika |
| collection | DOAJ |
| description | Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque was successfully isolated from soil of potato fields in Ngablak, Magelang. This research aimed to obtain the knowledge of P. vexans potency as a pathogen in potatoes, and also morphologically and molecularly identify P. vexans compared to the Oomycetes, Phytophthora and Pythium. Morphological identification was conducted by observing macroscopic colony pattern that grew on PDA medium for five days and microscopic observation on its hyphae, sporangia, papillate, and chlamydospore. Molecular identification was conducted using multigene analysis, ITS and LSU. The in vitro pathogenicity test was obtained by inoculating P. vexans inoculum to healthy potato leaves and tubers. Results of morphological observation showed that P. vexans had a stellate pattern, aseptate hyphae, ovoid shaped sporangium and completed with semipapillate. Chlamydospores were also found and often used for survival. This research revealed that the morphology characters of P. vexans were combination between the characteristics of Pythium and Phytophthora. Whereas, based on the molecular analysis using ITS and LSU, Phytopythium spp. was more closely related to Phytophthora spp. rather than Pythium spp. The pathogenicity test of P. vexans showed that it could infect the flesh of potato tubers by showing a brown lesions symptom |
| first_indexed | 2024-12-13T12:22:37Z |
| format | Article |
| id | doaj.art-f3293be0dd5049fdada46ee0a0cd2b1c |
| institution | Directory Open Access Journal |
| issn | 1410-1637 2548-4788 |
| language | English |
| last_indexed | 2024-12-13T12:22:37Z |
| publishDate | 2021-12-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Jurnal Perlindungan Tanaman Indonesia |
| spelling | doaj.art-f3293be0dd5049fdada46ee0a0cd2b1c2022-12-21T23:46:33ZengUniversitas Gadjah MadaJurnal Perlindungan Tanaman Indonesia1410-16372548-47882021-12-0125210.22146/jpti.6755630976First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in IndonesiaIslaminati Anna Santika0Ani Widiastuti1Arif Wibowo2Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55581 IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55581 IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55581 IndonesiaPhytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque was successfully isolated from soil of potato fields in Ngablak, Magelang. This research aimed to obtain the knowledge of P. vexans potency as a pathogen in potatoes, and also morphologically and molecularly identify P. vexans compared to the Oomycetes, Phytophthora and Pythium. Morphological identification was conducted by observing macroscopic colony pattern that grew on PDA medium for five days and microscopic observation on its hyphae, sporangia, papillate, and chlamydospore. Molecular identification was conducted using multigene analysis, ITS and LSU. The in vitro pathogenicity test was obtained by inoculating P. vexans inoculum to healthy potato leaves and tubers. Results of morphological observation showed that P. vexans had a stellate pattern, aseptate hyphae, ovoid shaped sporangium and completed with semipapillate. Chlamydospores were also found and often used for survival. This research revealed that the morphology characters of P. vexans were combination between the characteristics of Pythium and Phytophthora. Whereas, based on the molecular analysis using ITS and LSU, Phytopythium spp. was more closely related to Phytophthora spp. rather than Pythium spp. The pathogenicity test of P. vexans showed that it could infect the flesh of potato tubers by showing a brown lesions symptomhttps://jurnal.ugm.ac.id/jpti/article/view/67556itslsuoomycetespotatophytopythium |
| spellingShingle | Islaminati Anna Santika Ani Widiastuti Arif Wibowo First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia Jurnal Perlindungan Tanaman Indonesia its lsu oomycetes potato phytopythium |
| title | First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia |
| title_full | First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia |
| title_fullStr | First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia |
| title_full_unstemmed | First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia |
| title_short | First Report of Phytopythium vexans (de Barry) Abad, de Cock, Bala, Robideau, Lodhi & Lévesque Causing Potato Tuber Rot in Indonesia |
| title_sort | first report of phytopythium vexans de barry abad de cock bala robideau lodhi levesque causing potato tuber rot in indonesia |
| topic | its lsu oomycetes potato phytopythium |
| url | https://jurnal.ugm.ac.id/jpti/article/view/67556 |
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