Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age
Abstract Interferon-gamma release assays performance can be impaired by host-related, technical and environmental factors, but data in young children are limited. We performed a cross-sectional study of children < 5 years-of-age at risk of tuberculosis (TB), using QuantiFERON-TB Gold In-Tube (QFT...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24433-w |
| _version_ | 1811309278997250048 |
|---|---|
| author | Eneritz Velasco-Arnaiz Marta Batllori Manuel Monsonís Anna Valls María Ríos-Barnes Sílvia Simó-Nebot Anna Gamell Clàudia Fortuny Marc Tebruegge Antoni Noguera-Julian |
| author_facet | Eneritz Velasco-Arnaiz Marta Batllori Manuel Monsonís Anna Valls María Ríos-Barnes Sílvia Simó-Nebot Anna Gamell Clàudia Fortuny Marc Tebruegge Antoni Noguera-Julian |
| author_sort | Eneritz Velasco-Arnaiz |
| collection | DOAJ |
| description | Abstract Interferon-gamma release assays performance can be impaired by host-related, technical and environmental factors, but data in young children are limited. We performed a cross-sectional study of children < 5 years-of-age at risk of tuberculosis (TB), using QuantiFERON-TB Gold In-Tube (QFT-GIT) assays. The impact of the following was evaluated: (i) host-related [age; hematological parameters; erythrocyte sedimentation rate (ESR); C-reactive protein (CRP); and tobacco smoke exposure (TSE) based on serum cotinine concentrations], (ii) technical (pre-analytical delay) and (iii) environmental factors (annual season; monthly temperatures). Of 204 children, 35 (17.2%) were diagnosed with latent TB infection or TB disease. QFT-GIT results were indeterminate in 14 (6.9%) patients. In multivariate analysis, younger age and higher ESR were associated with lower positive control responses (beta: 0.247, p = 0.002 and − 0.204, p = 0.007, respectively), and increasing age was associated with lower rates of indeterminate QFT-GIT results [OR (95% CI) 0.948 (0.903–0.996) per month, p = 0.035]. In children with positive QFT-GIT results, average monthly temperatures correlated with antigen responses (r = 0.453, p = 0.020); also, antigen responses were lower in winter than in other seasons (p = 0.027). Serum cotinine concentrations determined in a subgroup of patients (n = 41) indicated TSE in 36 (88%), positive control responses being lower in children with TSE (p = 0.034). In children < 5 years-of-age, young age, elevated ESR, temperature, annual season and TSE can affect the performance of QFT-GIT assays. |
| first_indexed | 2024-04-13T09:39:29Z |
| format | Article |
| id | doaj.art-f32a6f35042a4dd288ef6f83d7d52b78 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T09:39:29Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f32a6f35042a4dd288ef6f83d7d52b782022-12-22T02:52:00ZengNature PortfolioScientific Reports2045-23222022-11-011211910.1038/s41598-022-24433-wHost, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of ageEneritz Velasco-Arnaiz0Marta Batllori1Manuel Monsonís2Anna Valls3María Ríos-Barnes4Sílvia Simó-Nebot5Anna Gamell6Clàudia Fortuny7Marc Tebruegge8Antoni Noguera-Julian9Malalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria; Servei d’Infectologia Pediàtrica, Institut de Recerca Sant Joan de DéuLaboratori de Bioquímica, Hospital Sant Joan de DéuServei de Microbiologia. Hospital Sant Joan de DéuLaboratori de Bioquímica, Hospital Sant Joan de DéuMalalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria; Servei d’Infectologia Pediàtrica, Institut de Recerca Sant Joan de DéuMalalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria; Servei d’Infectologia Pediàtrica, Institut de Recerca Sant Joan de DéuMalalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria; Servei d’Infectologia Pediàtrica, Institut de Recerca Sant Joan de DéuMalalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria; Servei d’Infectologia Pediàtrica, Institut de Recerca Sant Joan de DéuDepartment of Infection, Immunity and Inflammation, UCL Great Ormond Street Institute of Child Health, University College LondonMalalties Infeccioses i Resposta Inflamatòria Sistèmica en Pediatria; Servei d’Infectologia Pediàtrica, Institut de Recerca Sant Joan de DéuAbstract Interferon-gamma release assays performance can be impaired by host-related, technical and environmental factors, but data in young children are limited. We performed a cross-sectional study of children < 5 years-of-age at risk of tuberculosis (TB), using QuantiFERON-TB Gold In-Tube (QFT-GIT) assays. The impact of the following was evaluated: (i) host-related [age; hematological parameters; erythrocyte sedimentation rate (ESR); C-reactive protein (CRP); and tobacco smoke exposure (TSE) based on serum cotinine concentrations], (ii) technical (pre-analytical delay) and (iii) environmental factors (annual season; monthly temperatures). Of 204 children, 35 (17.2%) were diagnosed with latent TB infection or TB disease. QFT-GIT results were indeterminate in 14 (6.9%) patients. In multivariate analysis, younger age and higher ESR were associated with lower positive control responses (beta: 0.247, p = 0.002 and − 0.204, p = 0.007, respectively), and increasing age was associated with lower rates of indeterminate QFT-GIT results [OR (95% CI) 0.948 (0.903–0.996) per month, p = 0.035]. In children with positive QFT-GIT results, average monthly temperatures correlated with antigen responses (r = 0.453, p = 0.020); also, antigen responses were lower in winter than in other seasons (p = 0.027). Serum cotinine concentrations determined in a subgroup of patients (n = 41) indicated TSE in 36 (88%), positive control responses being lower in children with TSE (p = 0.034). In children < 5 years-of-age, young age, elevated ESR, temperature, annual season and TSE can affect the performance of QFT-GIT assays.https://doi.org/10.1038/s41598-022-24433-w |
| spellingShingle | Eneritz Velasco-Arnaiz Marta Batllori Manuel Monsonís Anna Valls María Ríos-Barnes Sílvia Simó-Nebot Anna Gamell Clàudia Fortuny Marc Tebruegge Antoni Noguera-Julian Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age Scientific Reports |
| title | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
| title_full | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
| title_fullStr | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
| title_full_unstemmed | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
| title_short | Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age |
| title_sort | host technical and environmental factors affecting quantiferon tb gold in tube performance in children below 5 years of age |
| url | https://doi.org/10.1038/s41598-022-24433-w |
| work_keys_str_mv | AT eneritzvelascoarnaiz hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT martabatllori hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT manuelmonsonis hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT annavalls hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT mariariosbarnes hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT silviasimonebot hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT annagamell hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT claudiafortuny hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT marctebruegge hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage AT antoninoguerajulian hosttechnicalandenvironmentalfactorsaffectingquantiferontbgoldintubeperformanceinchildrenbelow5yearsofage |