Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea
In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to de...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2022-08-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-NT-04-2022-0051.pdf |
| _version_ | 1817998859858608128 |
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| author | Diane Avalos-Ruiz Leonid N. Ten Chang-Kil Kim Seung-Yeol Lee Hee-Young Jung |
| author_facet | Diane Avalos-Ruiz Leonid N. Ten Chang-Kil Kim Seung-Yeol Lee Hee-Young Jung |
| author_sort | Diane Avalos-Ruiz |
| collection | DOAJ |
| description | In biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea. |
| first_indexed | 2024-04-14T03:00:57Z |
| format | Article |
| id | doaj.art-f32c32dfaea343c6be5532804c8b522b |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-14T03:00:57Z |
| publishDate | 2022-08-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-f32c32dfaea343c6be5532804c8b522b2022-12-22T02:15:56ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802022-08-0138440340910.5423/PPJ.NT.04.2022.00512335Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in KoreaDiane Avalos-Ruiz0Leonid N. Ten1Chang-Kil Kim2Seung-Yeol Lee3Hee-Young Jung4 School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea School of Applied Biosciences, Kyungpook National University, Daegu 41566, Korea School of Applied Biosciences, Kyungpook National University, Daegu 41566, KoreaIn biological particles such as Fusarium species, ice nucleation activity (INA) has been observed. Fusarium strains isolated from apple declined trees in Korea were identified with a multilocus sequence analysis using the tef1 and rpb1 genes. Droplet-freezing and tube-freezing assays were used to determine the INA of the strains, using Pseudomonas syringae pv. syringae KACC 21200 as a positive control and resulting in seven INA+ fungal strains that were identified as F. tricinctum (KNUF-21-F17, KNUF-21-F18, KNUF-21-F29, KNUF-21-F32, KNUF-21-F38, KNUF-21-F43, and KNUF-21-F44). The effect of Fusarium INA+ KNUF-21-F29 was compared to that of INA– strains on Chrysanthemum morifolium cv. Shinma explants. A higher callus formation and no-shoot formation were observed, suggesting that fungal INA could play a role in cold injuries and be a factor to consider in rapid apple decline. To the best of our knowledge, this is the first report of INA fungal strains isolated in Korea.http://www.ppjonline.org/upload/pdf/PPJ-NT-04-2022-0051.pdfcold injuryice nucleation activity |
| spellingShingle | Diane Avalos-Ruiz Leonid N. Ten Chang-Kil Kim Seung-Yeol Lee Hee-Young Jung Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea The Plant Pathology Journal cold injury ice nucleation activity |
| title | Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea |
| title_full | Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea |
| title_fullStr | Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea |
| title_full_unstemmed | Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea |
| title_short | Isolation and Identification of Ice Nucleation Active Strains from Rapid Apple Declined Trees in Korea |
| title_sort | isolation and identification of ice nucleation active strains from rapid apple declined trees in korea |
| topic | cold injury ice nucleation activity |
| url | http://www.ppjonline.org/upload/pdf/PPJ-NT-04-2022-0051.pdf |
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