Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis
Xinyu Yang, Xiumin Yang Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Xiumin Yang, Email YXM8092@163.comAbstract: Sporotrichosis is a common chronic fungal infection and the clinical manifestations are often untypi...
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| Formaat: | Artikel |
| Taal: | English |
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Dove Medical Press
2024-09-01
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| Reeks: | Clinical, Cosmetic and Investigational Dermatology |
| Onderwerpen: | |
| Online toegang: | https://www.dovepress.com/application-of-pcr-sequencing-and-next-generation-sequencing-in-the-di-peer-reviewed-fulltext-article-CCID |
| _version_ | 1827127778654289920 |
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| author | Yang X Yang X |
| author_facet | Yang X Yang X |
| author_sort | Yang X |
| collection | DOAJ |
| description | Xinyu Yang, Xiumin Yang Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Xiumin Yang, Email YXM8092@163.comAbstract: Sporotrichosis is a common chronic fungal infection and the clinical manifestations are often untypical. Diagnosis of sporotrichosis relies conventionally on fungal culture, histopathological examination, and species identification by molecular test. We reported that a 70-year-old man presented with a cutaneous lesion on the back of his right hand (present for 6 months). The cutaneous bacterial infection was diagnosed at a local hospital and the lesion had not improved. Physical examination revealed an infiltrative reddish plaque with purulent secretion and crusts. Histopathological examination revealed scattered round yeast cells in the dermis. Fungal culture revealed multiple, velvety, brown colonies on Sabouraud dextrose agar (SDA). Sporothrix globosa was identified by PCR-sequencing and next generation sequencing (NGS) method. Finally, a case of sporotrichosis caused by Sporothrix globosa was diagnosed by histopathological examination, mycological examination, and molecular identification. The patient was treated with oral itraconazole 400 mg/day for 2 months. The lesion was dramatically ameliorated.Keywords: sporotrichosis, PCR sequencing, next generation sequencing |
| first_indexed | 2025-03-20T15:33:55Z |
| format | Article |
| id | doaj.art-f32e6e7fb5934b0ca52e3dba8fa6bda2 |
| institution | Directory Open Access Journal |
| issn | 1178-7015 |
| language | English |
| last_indexed | 2025-03-20T15:33:55Z |
| publishDate | 2024-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Clinical, Cosmetic and Investigational Dermatology |
| spelling | doaj.art-f32e6e7fb5934b0ca52e3dba8fa6bda22024-09-03T17:20:50ZengDove Medical PressClinical, Cosmetic and Investigational Dermatology1178-70152024-09-01Volume 171981198395389Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of SporotrichosisYang XYang XXinyu Yang, Xiumin Yang Department of Dermatology, Beijing Tongren Hospital, Capital Medical University, Beijing, People’s Republic of ChinaCorrespondence: Xiumin Yang, Email YXM8092@163.comAbstract: Sporotrichosis is a common chronic fungal infection and the clinical manifestations are often untypical. Diagnosis of sporotrichosis relies conventionally on fungal culture, histopathological examination, and species identification by molecular test. We reported that a 70-year-old man presented with a cutaneous lesion on the back of his right hand (present for 6 months). The cutaneous bacterial infection was diagnosed at a local hospital and the lesion had not improved. Physical examination revealed an infiltrative reddish plaque with purulent secretion and crusts. Histopathological examination revealed scattered round yeast cells in the dermis. Fungal culture revealed multiple, velvety, brown colonies on Sabouraud dextrose agar (SDA). Sporothrix globosa was identified by PCR-sequencing and next generation sequencing (NGS) method. Finally, a case of sporotrichosis caused by Sporothrix globosa was diagnosed by histopathological examination, mycological examination, and molecular identification. The patient was treated with oral itraconazole 400 mg/day for 2 months. The lesion was dramatically ameliorated.Keywords: sporotrichosis, PCR sequencing, next generation sequencinghttps://www.dovepress.com/application-of-pcr-sequencing-and-next-generation-sequencing-in-the-di-peer-reviewed-fulltext-article-CCIDsporotrichosispcr sequencingnext generation sequencing |
| spellingShingle | Yang X Yang X Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis Clinical, Cosmetic and Investigational Dermatology sporotrichosis pcr sequencing next generation sequencing |
| title | Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis |
| title_full | Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis |
| title_fullStr | Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis |
| title_full_unstemmed | Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis |
| title_short | Application of PCR Sequencing and Next-Generation Sequencing in the Diagnosis of Sporotrichosis |
| title_sort | application of pcr sequencing and next generation sequencing in the diagnosis of sporotrichosis |
| topic | sporotrichosis pcr sequencing next generation sequencing |
| url | https://www.dovepress.com/application-of-pcr-sequencing-and-next-generation-sequencing-in-the-di-peer-reviewed-fulltext-article-CCID |
| work_keys_str_mv | AT yangx applicationofpcrsequencingandnextgenerationsequencinginthediagnosisofsporotrichosis AT yangx applicationofpcrsequencingandnextgenerationsequencinginthediagnosisofsporotrichosis |