Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study
The roots of <i>Salvia miltiorrhiza</i> (Danshen) is a precious herbal medicine used to treat cardiovascular diseases. This study establishes a high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method to quantify seven bioactive constituents from Danshen in ra...
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MDPI AG
2021-06-01
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| author | Yanli Zhang Weiliang Cui Xianghong Liu Ning Wang Wenru Kong Junyu Sui Huifen Li Shuqi Wang |
| author_facet | Yanli Zhang Weiliang Cui Xianghong Liu Ning Wang Wenru Kong Junyu Sui Huifen Li Shuqi Wang |
| author_sort | Yanli Zhang |
| collection | DOAJ |
| description | The roots of <i>Salvia miltiorrhiza</i> (Danshen) is a precious herbal medicine used to treat cardiovascular diseases. This study establishes a high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method to quantify seven bioactive constituents from Danshen in rat plasma simultaneously. Chromatographic separation is performed on an Agilent Eclipse Plus C18 column (150 mm × 2.1 mm, 5 μm), utilizing a gradient of acetonitrile and 0.2% formic acid aqueous solution as the mobile phase, at a flow rate of 0.6 mL/min. We conduct a tandem mass spectrometric detection with electrospray ionization (ESI) interface via multiple reaction monitoring (MRM) in both positive and negative ionization mode. Our results show that a linear relationship is established for each analyte of interest over the concentration range of 0.5–300 ng/mL with r ≥ 0.9976. The validated method is successfully used to compare the pharmacokinetic properties of crude and wine-processed Danshen extract orally administered to rats. <i>C<sub>max</sub></i> of tanshinone IIA, <i>C<sub>max</sub></i>, and <i>AUC</i><sub>0-<i>t</i></sub> of dihydrotanshinone I decrease significantly (<i>p</i> < 0.05) in the wine-processed group. No significant changes for other compounds are observed. These results might provide meaningful information for the further application of wine-processed Danshen and understanding of wine-processing mechanisms. |
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| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T09:57:14Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Separations |
| spelling | doaj.art-f32ef0ad59f2412e9d8bc0ffac29bf712023-11-22T02:16:52ZengMDPI AGSeparations2297-87392021-06-01879310.3390/separations8070093Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic StudyYanli Zhang0Weiliang Cui1Xianghong Liu2Ning Wang3Wenru Kong4Junyu Sui5Huifen Li6Shuqi Wang7Department of Pharmacy, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Jinan 250012, ChinaShandong Institute for Food and Drug Control, Jinan 250101, ChinaDepartment of Pharmacy, Qilu Hospital of Shandong University, Cheeloo College of Medicine, Jinan 250012, ChinaSchool of Pharmaceutical Sciences, Shandong University, Jinan 250012, ChinaSchool of Pharmaceutical Sciences, Shandong University, Jinan 250012, ChinaCollege of Agriculture & Life Science, University of Wisconsin, Madison, WI 53706, USACollege of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, ChinaSchool of Pharmaceutical Sciences, Shandong University, Jinan 250012, ChinaThe roots of <i>Salvia miltiorrhiza</i> (Danshen) is a precious herbal medicine used to treat cardiovascular diseases. This study establishes a high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS/MS) method to quantify seven bioactive constituents from Danshen in rat plasma simultaneously. Chromatographic separation is performed on an Agilent Eclipse Plus C18 column (150 mm × 2.1 mm, 5 μm), utilizing a gradient of acetonitrile and 0.2% formic acid aqueous solution as the mobile phase, at a flow rate of 0.6 mL/min. We conduct a tandem mass spectrometric detection with electrospray ionization (ESI) interface via multiple reaction monitoring (MRM) in both positive and negative ionization mode. Our results show that a linear relationship is established for each analyte of interest over the concentration range of 0.5–300 ng/mL with r ≥ 0.9976. The validated method is successfully used to compare the pharmacokinetic properties of crude and wine-processed Danshen extract orally administered to rats. <i>C<sub>max</sub></i> of tanshinone IIA, <i>C<sub>max</sub></i>, and <i>AUC</i><sub>0-<i>t</i></sub> of dihydrotanshinone I decrease significantly (<i>p</i> < 0.05) in the wine-processed group. No significant changes for other compounds are observed. These results might provide meaningful information for the further application of wine-processed Danshen and understanding of wine-processing mechanisms.https://www.mdpi.com/2297-8739/8/7/93<i>Salvia miltiorrhiza</i>HPLC-MS/MSpharmacokineticswine-processed |
| spellingShingle | Yanli Zhang Weiliang Cui Xianghong Liu Ning Wang Wenru Kong Junyu Sui Huifen Li Shuqi Wang Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study Separations <i>Salvia miltiorrhiza</i> HPLC-MS/MS pharmacokinetics wine-processed |
| title | Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study |
| title_full | Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study |
| title_fullStr | Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study |
| title_full_unstemmed | Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study |
| title_short | Simultaneous Determination of Seven Bioactive Constituents from <i>Salvia miltiorrhiza</i> in Rat Plasma by HPLC-MS/MS: Application to a Comparative Pharmacokinetic Study |
| title_sort | simultaneous determination of seven bioactive constituents from i salvia miltiorrhiza i in rat plasma by hplc ms ms application to a comparative pharmacokinetic study |
| topic | <i>Salvia miltiorrhiza</i> HPLC-MS/MS pharmacokinetics wine-processed |
| url | https://www.mdpi.com/2297-8739/8/7/93 |
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