Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial
Vitamin D could be beneficial for healthy ageing in humans. We previously found that vitamin D supplementation may slow down epigenetic ageing in young African American adults. We tested new epigenetic clocks developed for neonates among a multiethnic population, and tested the hypothesis that mater...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-08-01
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| Series: | Epigenetics |
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| Online Access: | http://dx.doi.org/10.1080/15592294.2020.1734148 |
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| author | Li Chen Carol L. Wagner Yanbin Dong Xiaoling Wang Judith R. Shary Ying Huang Bruce W. Hollis Haidong Zhu |
| author_facet | Li Chen Carol L. Wagner Yanbin Dong Xiaoling Wang Judith R. Shary Ying Huang Bruce W. Hollis Haidong Zhu |
| author_sort | Li Chen |
| collection | DOAJ |
| description | Vitamin D could be beneficial for healthy ageing in humans. We previously found that vitamin D supplementation may slow down epigenetic ageing in young African American adults. We tested new epigenetic clocks developed for neonates among a multiethnic population, and tested the hypothesis that maternal vitamin D supplementation would slow down the epigenetic gestational age acceleration (GAA) in newborn babies. Ninety-two pregnant women (aged 29.6 ± 4.8 y; 21% African Americans, 28% Hispanics) were randomized to receive 4000 IU/day vitamin D3 or placebo, plus prenatal vitamins containing 400 IU vitamin D3 during pregnancy in a randomized controlled trial (RCT). Cord blood genome-wide methylation analysis was performed on the Illumina Infinium MethylationEPIC Beadchip. DNA methylation gestational age was calculated based on two calculations developed by Knight and Bohlin. DNA methylation gestational ages calculated by Knight’s clock and Bohlin’ clock were highly correlated with the gestational age in the placebo group (correlation coefficients = 0.88, p s< 0.001, respectively). GAA was associated with higher birth weight (p = 0.039). In the entire cohort, vitamin D3 supplementation was not associated with GAA (p > 0.05). However, vitamin D3 supplementation decreased GAA by both Knight’s clock (β = −0.89, p = 0.047) and Bohlin’s clock (β = −0.71, p = 0.005) in the African American participants. Maternal vitamin D3 supplementation may slow down the epigenetic gestational ageing process in African American neonates. Long-term follow-up studies are warranted to determine the role of epigenetic age acceleration in the growth and development of offspring. |
| first_indexed | 2024-03-11T23:07:00Z |
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| id | doaj.art-f32f8d1f64764d259bf6cf5eb1c811c6 |
| institution | Directory Open Access Journal |
| issn | 1559-2294 1559-2308 |
| language | English |
| last_indexed | 2024-03-11T23:07:00Z |
| publishDate | 2020-08-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Epigenetics |
| spelling | doaj.art-f32f8d1f64764d259bf6cf5eb1c811c62023-09-21T13:09:23ZengTaylor & Francis GroupEpigenetics1559-22941559-23082020-08-0115883084010.1080/15592294.2020.17341481734148Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled TrialLi Chen0Carol L. Wagner1Yanbin Dong2Xiaoling Wang3Judith R. Shary4Ying Huang5Bruce W. Hollis6Haidong Zhu7Augusta UniversityMedical University of South CarolinaAugusta UniversityAugusta UniversityMedical University of South CarolinaAugusta UniversityMedical University of South CarolinaAugusta UniversityVitamin D could be beneficial for healthy ageing in humans. We previously found that vitamin D supplementation may slow down epigenetic ageing in young African American adults. We tested new epigenetic clocks developed for neonates among a multiethnic population, and tested the hypothesis that maternal vitamin D supplementation would slow down the epigenetic gestational age acceleration (GAA) in newborn babies. Ninety-two pregnant women (aged 29.6 ± 4.8 y; 21% African Americans, 28% Hispanics) were randomized to receive 4000 IU/day vitamin D3 or placebo, plus prenatal vitamins containing 400 IU vitamin D3 during pregnancy in a randomized controlled trial (RCT). Cord blood genome-wide methylation analysis was performed on the Illumina Infinium MethylationEPIC Beadchip. DNA methylation gestational age was calculated based on two calculations developed by Knight and Bohlin. DNA methylation gestational ages calculated by Knight’s clock and Bohlin’ clock were highly correlated with the gestational age in the placebo group (correlation coefficients = 0.88, p s< 0.001, respectively). GAA was associated with higher birth weight (p = 0.039). In the entire cohort, vitamin D3 supplementation was not associated with GAA (p > 0.05). However, vitamin D3 supplementation decreased GAA by both Knight’s clock (β = −0.89, p = 0.047) and Bohlin’s clock (β = −0.71, p = 0.005) in the African American participants. Maternal vitamin D3 supplementation may slow down the epigenetic gestational ageing process in African American neonates. Long-term follow-up studies are warranted to determine the role of epigenetic age acceleration in the growth and development of offspring.http://dx.doi.org/10.1080/15592294.2020.1734148maternal vitamin d3 supplementationcholecalciferoldna methylationgestational ageepigenetic clockprenatal nutritionmultiethnic population |
| spellingShingle | Li Chen Carol L. Wagner Yanbin Dong Xiaoling Wang Judith R. Shary Ying Huang Bruce W. Hollis Haidong Zhu Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial Epigenetics maternal vitamin d3 supplementation cholecalciferol dna methylation gestational age epigenetic clock prenatal nutrition multiethnic population |
| title | Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial |
| title_full | Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial |
| title_fullStr | Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial |
| title_full_unstemmed | Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial |
| title_short | Effects of Maternal Vitamin D3 Supplementation on Offspring Epigenetic Clock of Gestational Age at Birth: A Post-hoc Analysis of a Randomized Controlled Trial |
| title_sort | effects of maternal vitamin d3 supplementation on offspring epigenetic clock of gestational age at birth a post hoc analysis of a randomized controlled trial |
| topic | maternal vitamin d3 supplementation cholecalciferol dna methylation gestational age epigenetic clock prenatal nutrition multiethnic population |
| url | http://dx.doi.org/10.1080/15592294.2020.1734148 |
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