Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China
Abstract Endophytic bacteria are one of the symbiotic microbial groups closely related to host algae. However, less research on the endophytic bacteria of marine algae. In this study, the endophytic bacterial community of Sargassum thunbergii was investigated using the culture method and high-throug...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-04-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-024-01688-2 |
| _version_ | 1797209108372258816 |
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| author | Yang Li Jing Wang Tao Sun Xinlong Yu Zhibo Yang Yayun Zhao Xuexi Tang Hui Xiao |
| author_facet | Yang Li Jing Wang Tao Sun Xinlong Yu Zhibo Yang Yayun Zhao Xuexi Tang Hui Xiao |
| author_sort | Yang Li |
| collection | DOAJ |
| description | Abstract Endophytic bacteria are one of the symbiotic microbial groups closely related to host algae. However, less research on the endophytic bacteria of marine algae. In this study, the endophytic bacterial community of Sargassum thunbergii was investigated using the culture method and high-throughput sequencing. Thirty-nine endophytic bacterial strains, belonging to two phyla, five genera and sixteen species, were isolated, and Firmicutes, Bacillus and Metabacillus indicus were the dominant taxa at the phylum, genus and species level, respectively. High-throughput sequencing revealed 39 phyla and 574 genera of endophytic bacteria, and the dominant phylum was Proteobacteria, while the dominant genus was Ralstonia. The results also indicated that the endophytic bacteria of S. thunbergii included various groups with nitrogen fixation, salt tolerance, pollutant degradation, and antibacterial properties but also contained some pathogenic bacteria. Additionally, the endophytic bacterial community shared a large number of groups with the epiphytic bacteria and bacteria in the surrounding seawater, but the three groups of samples could be clustered separately. In conclusion, there are a variety of functional endophytic bacteria living in S. thunbergii, and the internal condition of algae is a selective factor for the formation of endophytic bacterial communities. This study enriched the database of endophytic bacteria in marine macroalgae, paving the way for further understanding of the interrelationships between endophytic bacteria, macroalgae, and the environment. |
| first_indexed | 2024-04-24T09:49:27Z |
| format | Article |
| id | doaj.art-f33259588fdc45478b89a3272a76d16d |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-04-24T09:49:27Z |
| publishDate | 2024-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-f33259588fdc45478b89a3272a76d16d2024-04-14T11:30:14ZengSpringerOpenAMB Express2191-08552024-04-0114111410.1186/s13568-024-01688-2Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in ChinaYang Li0Jing Wang1Tao Sun2Xinlong Yu3Zhibo Yang4Yayun Zhao5Xuexi Tang6Hui Xiao7College of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaCollege of Marine Life Sciences, Ocean University of ChinaAbstract Endophytic bacteria are one of the symbiotic microbial groups closely related to host algae. However, less research on the endophytic bacteria of marine algae. In this study, the endophytic bacterial community of Sargassum thunbergii was investigated using the culture method and high-throughput sequencing. Thirty-nine endophytic bacterial strains, belonging to two phyla, five genera and sixteen species, were isolated, and Firmicutes, Bacillus and Metabacillus indicus were the dominant taxa at the phylum, genus and species level, respectively. High-throughput sequencing revealed 39 phyla and 574 genera of endophytic bacteria, and the dominant phylum was Proteobacteria, while the dominant genus was Ralstonia. The results also indicated that the endophytic bacteria of S. thunbergii included various groups with nitrogen fixation, salt tolerance, pollutant degradation, and antibacterial properties but also contained some pathogenic bacteria. Additionally, the endophytic bacterial community shared a large number of groups with the epiphytic bacteria and bacteria in the surrounding seawater, but the three groups of samples could be clustered separately. In conclusion, there are a variety of functional endophytic bacteria living in S. thunbergii, and the internal condition of algae is a selective factor for the formation of endophytic bacterial communities. This study enriched the database of endophytic bacteria in marine macroalgae, paving the way for further understanding of the interrelationships between endophytic bacteria, macroalgae, and the environment.https://doi.org/10.1186/s13568-024-01688-2Sargassum thunbergiiEndophytic bacteriaCommunity structureCulture methodHigh-throughput sequencing |
| spellingShingle | Yang Li Jing Wang Tao Sun Xinlong Yu Zhibo Yang Yayun Zhao Xuexi Tang Hui Xiao Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China AMB Express Sargassum thunbergii Endophytic bacteria Community structure Culture method High-throughput sequencing |
| title | Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China |
| title_full | Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China |
| title_fullStr | Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China |
| title_full_unstemmed | Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China |
| title_short | Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China |
| title_sort | community structure of endophytic bacteria of sargassum thubergii in the intertidal zone of qingdao in china |
| topic | Sargassum thunbergii Endophytic bacteria Community structure Culture method High-throughput sequencing |
| url | https://doi.org/10.1186/s13568-024-01688-2 |
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